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YY1 antibody | CD02/2B2

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Anti YY1 Antibody, clone CD02/2B2 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti YY1 Antibody, clone CD02/2B2 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti YY1 Antibody, clone CD02/2B2 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti YY1 Antibody, clone CD02/2B2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with YY1 antibody (1/1,000, VMA00573) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to YY1 (molecular weight 62 kDa).
Anti YY1 Antibody, clone CD02/2B2 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of YY1 from Jurkat whole cell lysate. The Input lane is 6.25% input; the YY1-IP lane is the immunoprecipitation with the YY1 antibody (5 µg, VMA00573); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (5 µg, MCA928). Western blot analysis was performed using YY1 antibody (1/1000, VMA00573) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to YY1.
Anti YY1 Antibody, clone CD02/2B2 (PrecisionAb Monoclonal Antibody) gallery image 3
HeLa cells stained with Mouse anti Human YY1 antibody (VMA00573) at a 1/100 dilution followed by detection with an Alexa Fluor 488 conjugated Goat anti Mouse IgG (H/L) antibody at a 1/400 dilution (green) and PureBlu™ DAPI (1351303) (blue) as a nuclear counterstain.

Mouse anti YY1

Product Type
PrecisionAb Monoclonal
Clone
CD02/2B2
Isotype
IgG1
Format
Purified
Specificity
YY1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00573
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SDS Safety Datasheet SDS
IF IP WB 100 µl loader
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loader

Mouse anti Human YY1 antibody recognizes the transcriptional repressor protein YY1, also known as INO80 complex subunit S or Yin and Yang 1 protein.

The protein YY1 is a ubiquitously-expressed zinc finger transcription factor which can act as an activator or repressor of transcription under different circumstances (Galloway et al. 2017). In addition to transcriptional regulation, YY1 is involved with cell proliferation, chromatin remodeling and apoptosis. Many targets of YY1, for example ERBB2 and p53, are involved with cancer pathogenesis (Kaufhold et al. 2016). Accordingly, YY1 is overexpressed in multiple cancer types, and overexpression is correlated with poor prognosis (Cho and Bonavida 2017). YY1 may be inhibited by siRNA, proteasome inhibitors, and a variety of other factors. Inhibition of YY1 has been suggested as a potential target in cancer (Bonavida 2017).		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti YY1 detects a band of approximately 62 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human YY1 (amino acids 1 - 414)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P25490
Entrez Gene
YY1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0008270 zinc ion binding
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016363 nuclear matrix

VMA00573

100004115 64584199 64607219

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