YY1 antibody | CD02/2B2

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Mouse anti YY1

Product Type
PrecisionAb Monoclonal
Clone
CD02/2B2
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00573
Datasheet
IP WB
MSDS
100 µl loader

Mouse anti Human YY1 antibody recognizes the transcriptional repressor protein YY1, also known as INO80 complex subunit S or Yin and Yang 1 protein.

The protein YY1 is a ubiquitously-expressed zinc finger transcription factor which can act as an activator or repressor of transcription under different circumstances (Galloway et al. 2017). In addition to transcriptional regulation, YY1 is involved with cell proliferation, chromatin remodeling and apoptosis. Many targets of YY1, for example ERBB2 and p53, are involved with cancer pathogenesis (Kaufhold et al. 2016). Accordingly, YY1 is overexpressed in multiple cancer types, and overexpression is correlated with poor prognosis (Cho and Bonavida 2017). YY1 may be inhibited by siRNA, proteasome inhibitors, and a variety of other factors. Inhibition of YY1 has been suggested as a potential target in cancer (Bonavida 2017).

Product Details

Target Species
Human
Western Blotting
Anti YY1 detects a band of approximately 62 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human YY1 (amino acids 1 - 414)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P25490
Entrez Gene
YY1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0008270 zinc ion binding
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016363 nuclear matrix
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of YY1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 1/1000
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml