Vimentin antibody | AbD2701
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Human anti Vimentin
- Product Type
- PrecisionAb Monoclonal
- HuCAL Fab bivalent
- Target Species
- Western Blotting
- Anti vimentin antibody detects a band of approximately 54 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
- Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Formalin fixed, paraffin-embedded human lymphoma tissue
- Approx. Protein Concentrations
- F(ab) concentration 0.5 mg/ml
- For research purposes only
- 12 months from date of despatch
- PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1||1/25||1/50|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Mouse anti Penta Histidine Tag:HRP||MCA5995P||E WB||125 µl||Log in|
|List Price||Your Price|
|Description||Mouse anti Penta Histidine Tag:HRP|
|Goat anti Human IgG F(ab')2:HRP||STAR126P||E WB||0.5 ml||Log in|
|List Price||Your Price|
|Description||Goat anti Human IgG F(ab')2:HRP|
References for Vimentin antibody
Jarutat, T. et al. (2007) Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue.
Biol Chem. 388 (6): 651-8.
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