Vimentin antibody | AbD2701
Human anti Human vimentin antibody recognizes human vimentin, a class III intermediate filament of ~ 54kDa. Vimentin is preferentially expressed in mesenchymal tissues, and within the immune system is retained throughout T-cell development, with decreasing expression in B lymphocytes with maturation.
In lymphatic tissues vimentin expression is seen in marginal zone areas, but not in follicle centers. A lack of vimentin expression in B cell lymphoma is indicative of follicular center origin.
Human anti Human vimentin antibody recognizes vimentin as a band of ~54 kDa in a number of human cell line lysates by western blotting under reducing conditions.
In lymphatic tissues vimentin expression is seen in marginal zone areas, but not in follicle centers. A lack of vimentin expression in B cell lymphoma is indicative of follicular center origin.
Human anti Human vimentin antibody recognizes vimentin as a band of ~54 kDa in a number of human cell line lysates by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti vimentin antibody detects a band of approximately 54 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
- Preparation
- Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Formalin fixed, paraffin-embedded human lymphoma tissue
- Approx. Protein Concentrations
- F(ab) concentration 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P08670 Related reagents
- Entrez Gene
- VIM Related reagents
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
- Regulatory
- For research purposes only
Applications of Vimentin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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|---|---|---|---|---|---|
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
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Product Specific References
References for Vimentin antibody
-
Jarutat, T. et al. (2007) Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue.
Biol Chem. 388 (6): 651-8.
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