Vimentin antibody | AbD2701
KO Validated
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WB ResetHuman anti Vimentin
- Product Type
- PrecisionAb Monoclonal
- Clone
- AbD2701
- Isotype
- HuCAL Fab bivalent
- Format
- Purified
- Specificity
- Vimentin
Antibody Quality and Performance Guarantee
| Human anti Human vimentin antibody recognizes human vimentin, a class III intermediate filament of ~ 54kDa. Vimentin is preferentially expressed in mesenchymal tissues, and within the immune system is retained throughout T-cell development, with decreasing expression in B lymphocytes with maturation. In lymphatic tissues vimentin expression is seen in marginal zone areas, but not in follicle centers. A lack of vimentin expression in B cell lymphoma is indicative of follicular center origin. Human anti Human vimentin antibody recognizes vimentin as a band of ~54 kDa in a number of human cell line lysates by western blotting under reducing conditions. |
|
- Target Species
- Human
- Western Blotting
- Anti vimentin antibody detects a band of approximately 54 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
- Preparation
- Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Formalin fixed, paraffin-embedded human lymphoma tissue
- Approx. Protein Concentrations
- F(ab) concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 1 | |
1/25 | 1/50 |
| Immunoprecipitation | |
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| Western Blotting | |
1/1000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
- Histology Positive Control Tissue
- Tonsil
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|---|---|---|---|---|---|---|---|
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
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| Description | Goat anti Human IgG F(ab')2:HRP | ||||||
References for Vimentin antibody
-
Jarutat, T. et al. (2007) Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue.
Biol Chem. 388 (6): 651-8.
VMA00008
080714
163853
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