UPF1 antibody | AB03/4D5

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Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with UPF1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 77 second exposure. Arrow points to UPF1 (molecular weight 143 kDa).

Mouse anti UPF1

UPF1 antibody recognizes UPF1, also known as RENT1, NORF1, regulator of nonsense transcripts 1 or delta helicase.

Product Type: PrecisionAb™ Monoclonal
Clone: AB03/4D5
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00627 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human UPF1 antibody recognizes UPF1, also known as RENT1, NORF1, regulator of nonsense transcripts 1 or delta helicase.

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2014).

Mouse anti Human UPF1 antibody detects a band of 143 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Mouse anti UPF1 antibody detects a band of approximately 143 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

E. coli-derived recombinant human UPF1 (aa 630-1129)

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Guarantee: As supplied, 12 months from date of despatch

More Information

UniProt: Q92900 Related reagents
Entrez Gene: UPF1 Related reagents
GO Terms: GO:0006260 DNA replication; GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; GO:0005515 protein binding; GO:0000785 chromatin; GO:0000932 cytoplasmic mRNA processing body; GO:0003677 DNA binding; GO:0003682 chromatin binding; GO:0003723 RNA binding; GO:0004004 ATP-dependent RNA helicase activity; GO:0005524 ATP binding; GO:0006281 DNA repair; GO:0006406 mRNA export from nucleus; GO:0006449 regulation of translational termination; GO:0007049 cell cycle; GO:0008270 zinc ion binding; GO:0071044 histone mRNA catabolic process
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of UPF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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