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UPF1 antibody | AB03/4D5

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Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb™ Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with UPF1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 77 second exposure. Arrow points to UPF1 (molecular weight 143 kDa).

Mouse anti UPF1

UPF1 antibody recognizes UPF1, also known as RENT1, NORF1, regulator of nonsense transcripts 1 or delta helicase.

UPF1 Antibody with Control Lysate

UPF1 antibody recognizes UPF1, also known as RENT1, NORF1, regulator of nonsense transcripts 1 or delta helicase.

Product Type
PrecisionAb™ Monoclonal
Clone
AB03/4D5
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00627 WB 100 µl
VMA00627KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human UPF1 antibody recognizes UPF1, also known as RENT1, NORF1, regulator of nonsense transcripts 1 or delta helicase.

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2014).

Mouse anti Human UPF1 antibody detects a band of 143 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human UPF1 (aa 630-1129)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q92900 Related reagents
Entrez Gene
UPF1 Related reagents
GO Terms
GO:0006260 DNA replication
GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0005515 protein binding
GO:0000785 chromatin
GO:0000932 cytoplasmic mRNA processing body
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003723 RNA binding
GO:0004004 ATP-dependent RNA helicase activity
GO:0005524 ATP binding
GO:0006281 DNA repair
GO:0006406 mRNA export from nucleus
GO:0006449 regulation of translational termination
GO:0007049 cell cycle
GO:0008270 zinc ion binding
GO:0071044 histone mRNA catabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of UPF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Mouse anti UPF1 antibody detects a band of approximately 143 kDa in HEK293 cell lysates
Western Blotting
Mouse anti UPF1 antibody detects a band of approximately 143 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
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