UPF1 antibody | AB03/4D5

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Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with UPF1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 77 second exposure. Arrow points to UPF1 (molecular weight 143 kDa).

Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of UPF1 from HEK293 whole cell lysate. The Input lane is 10% input; the UPF1-IP lane is the immunoprecipitation with the UPF1 antibody (2.5 µg, **VMA00627**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA929). Western blot analysis was performed using UPF1 antibody (1/1,000, **VMA00627**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to UPF1.

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WB IP

Mouse anti UPF1

Product Type: PrecisionAb Monoclonal
Clone: AB03/4D5
Isotype: IgG2a
Format: Purified
Specificity: UPF1

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00627 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human UPF1 antibody recognizes UPF1, also known as RENT1, NORF1, regulator of nonsense transcripts 1 or delta helicase.

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2014).

Mouse anti Human UPF1 antibody detects a band of 143 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti UPF1 antibody detects a band of approximately 143 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human UPF1 (aa 630-1129)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q92900
Entrez Gene: UPF1
GO Terms: GO:0006260 DNA replication; GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; GO:0005515 protein binding; GO:0000785 chromatin; GO:0000932 cytoplasmic mRNA processing body; GO:0003677 DNA binding; GO:0003682 chromatin binding; GO:0003723 RNA binding; GO:0004004 ATP-dependent RNA helicase activity; View More GO Terms; GO:0005524 ATP binding; GO:0006281 DNA repair; GO:0006406 mRNA export from nucleus; GO:0006449 regulation of translational termination; GO:0007049 cell cycle; GO:0008270 zinc ion binding; GO:0071044 histone mRNA catabolic process