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UPF1 antibody | AB03/4D5

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Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with UPF1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 77 second exposure. Arrow points to UPF1 (molecular weight 143 kDa).
Anti UPF1 Antibody, clone AB03/4D5 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of UPF1 from HEK293 whole cell lysate. The Input lane is 10% input; the UPF1-IP lane is the immunoprecipitation with the UPF1 antibody (2.5 µg, VMA00627); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA929). Western blot analysis was performed using UPF1 antibody (1/1,000, VMA00627) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to UPF1.

Mouse anti UPF1

Product Type
PrecisionAb Monoclonal
Clone
AB03/4D5
Isotype
IgG2a
Format
Purified
Specificity
UPF1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00627
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti Human UPF1 antibody recognizes UPF1, also known as RENT1, NORF1, regulator of nonsense transcripts 1 or delta helicase.

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2014).

Mouse anti Human UPF1 antibody detects a band of 143 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti UPF1 antibody detects a band of approximately 143 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human UPF1 (aa 630-1129)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q92900
Entrez Gene
UPF1
GO Terms
GO:0006260 DNA replication
GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0005515 protein binding
GO:0000785 chromatin
GO:0000932 cytoplasmic mRNA processing body
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003723 RNA binding
GO:0004004 ATP-dependent RNA helicase activity
GO:0005524 ATP binding
GO:0006281 DNA repair
GO:0006406 mRNA export from nucleus
GO:0006449 regulation of translational termination
GO:0007049 cell cycle
GO:0008270 zinc ion binding
GO:0071044 histone mRNA catabolic process

VMA00627

170517

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