STAT5 antibody | OTI9F7
Mouse anti STAT5
STAT5 antibody recognizes the signal transducer and activator of transcription 5A/5B. The antibody has been validated for western blotting using whole cell lysates.
STAT5 Antibody with Control Lysate
STAT5 antibody recognizes the signal transducer and activator of transcription 5A/5B. The antibody has been validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI9F7
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00711 | WB | 100 µl | ||||
| VMA00711KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human STAT5 antibody recognizes the signal transducer and activator of transcription 5A/5B.
Signal transducer and activator of transcription 5 (STAT5) refers to two highly related proteins, STAT5A and STAT5B, which are part of the STAT family of transcription factors. In response to cytokines (IL2, IL4) and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. It has been shown that STAT5 is involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Though STAT5A and STAT5B are encoded by separate genes, the proteins are 90% identical at the amino acid level. Aberrant STAT5 activity has been shown to be closely connected to a wide range of human cancers (provided by RefSeq, Jul 2008).
Mouse anti Human STAT5 detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Signal transducer and activator of transcription 5 (STAT5) refers to two highly related proteins, STAT5A and STAT5B, which are part of the STAT family of transcription factors. In response to cytokines (IL2, IL4) and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. It has been shown that STAT5 is involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Though STAT5A and STAT5B are encoded by separate genes, the proteins are 90% identical at the amino acid level. Aberrant STAT5 activity has been shown to be closely connected to a wide range of human cancers (provided by RefSeq, Jul 2008).
Mouse anti Human STAT5 detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti STAT5 detects a band of approximately 90 kDa in K562 cell lysates
- Product Form
- Purified IgG - Liquid
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody affinity purified on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
<50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
<50% Glycerol - Immunogen
- Full length recombinant human STAT5A produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P42229 Related reagents
- P51692 Related reagents
- Entrez Gene
- STAT5A Related reagents
- STAT5B Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000255 allantoin metabolic process
- GO:0005509 calcium ion binding
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0006101 citrate metabolic process
- GO:0006103 2-oxoglutarate metabolic process
- GO:0006105 succinate metabolic process
- GO:0006107 oxaloacetate metabolic process
- GO:0006549 isoleucine metabolic process
- GO:0006573 valine metabolic process
- GO:0006600 creatine metabolic process
- GO:0006631 fatty acid metabolic process
- GO:0019530 taurine metabolic process
- GO:0046449 creatinine metabolic process
- GO:0032355 response to estradiol stimulus
- GO:0035259 glucocorticoid receptor binding
- GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of STAT5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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