Somatostatin antibody | YC7
Rat anti Human Somatostatin antibody, clone YC7 (8330-0009) used to detect neuronal tissue in Zebrafish whole-mounts by immunofluorescence.
Image caption:
Whole-mount immunofluorescence staining of the zebrafish larvae (a, b) and juvenile zebrafish gut (c, d) using antibody against galanin (a–d) and neuronal marker Hu (a, d), somatostatin (Sst) (c). Red fluorescence protein (RFP) marked mnx1+ population of β cells (b, b'). Arrows show galaninergic neurons in the autonomic ganglion. In the primary islet of the endocrine pancreas, galanin-IR fibers formed a very dense network (arrowheads). CrG cranial ganglia, E eye, I intestine, Me mesencephalon, Rho rhombencephalon, Y yolk. Scale bars 100 μm
From: Podlasz P, Jakimiuk A, Chmielewska-Krzesinska M, Kasica N, Nowik N, Kaleczyc J.
Galanin regulates blood glucose level in the zebrafish: a morphological and functional study.
Histochem Cell Biol. 2016 Jan;145(1):105-17.
Rat anti Human Somatostatin antibody, clone YC7 (8330-0009) used to detect neuronal tissue in Zebrafish whole-mounts by immunofluorescence.
Image caption:
Whole-mount immunofluorescence staining of the zebrafish larvae (a, b) and juvenile zebrafish gut (c, d) using antibody against galanin (a–d) and neuronal marker Hu (a, d), somatostatin (Sst) (c). Red fluorescence protein (RFP) marked mnx1+ population of β cells (b, b'). Arrows show galaninergic neurons in the autonomic ganglion. In the primary islet of the endocrine pancreas, galanin-IR fibers formed a very dense network (arrowheads). CrG cranial ganglia, E eye, I intestine, Me mesencephalon, Rho rhombencephalon, Y yolk. Scale bars 100 μm
From: Podlasz P, Jakimiuk A, Chmielewska-Krzesinska M, Kasica N, Nowik N, Kaleczyc J.
Galanin regulates blood glucose level in the zebrafish: a morphological and functional study.
Histochem Cell Biol. 2016 Jan;145(1):105-17.
Rat anti Human Somatostatin antibody, clone YC7 (8330-0009) used to detect neuronal tissue in Zebrafish larvae whole-mounts by immunofluorescence.
Image caption:
Whole-mount immunofluorescence staining of the zebrafish larvae (a, b, d), juvenile (c, e), and adult (f) guts using antibody against galanin (a–c, e–f), cholinergic marker—choline acetyltransferase (ChAT) (a), δ cell marker somatostatin (b–d). RFP marked mnx1+ population of β cells in the transgenic line of the zebrafish (d, e). Arrows point to galaninergic neurons in autonomic ganglia. Arrowheads show the primary islet of the endocrine pancreas, and hollow arrows point to the secondary islets. a There was no ChAT immunoreactivity in the cells and fibers associated with the pancreas and intestine. ChAT-IR neurons were observed only in the brain and spinal cord (not shown). Galanin-IR fibers were also visible, projecting from the autonomic ganglion to the intestine. c, d Pancreatic islets at a higher magnification. Somatostatin did not colocalize with β cell marker (d). Delta cells were very intensely supplied by galaninergic fibers (c, d). Note that the secondary islets did not contain RFP+ cells until 60 dpf (e). Dashed line indicates the pancreas. I intestine, L liver, Rho rhombencephalon. Scale bars 100 μm, except d = 50 μm
From: Podlasz P, Jakimiuk A, Chmielewska-Krzesinska M, Kasica N, Nowik N, Kaleczyc J.
Galanin regulates blood glucose level in the zebrafish: a morphological and functional study.
Histochem Cell Biol. 2016 Jan;145(1):105-17.
Rat anti Human Somatostatin antibody, clone YC7 (8330-0009) used to detect neuronal tissue in Zebrafish tissues by immunofluorescence.
Image caption:
Section from the adult pancreas stained with antibody against galanin (a–c) and Hu (b), and somatostatin (c). RFP marked mnx1+ population of β cells in the transgenic line of the zebrafish (a–c). RFP+ cells were present mostly only in one (primary) islet, whereas somatostatin-positive cells were located in all the islets (a–c). Ganglion containing galanin-IR neurons is visible outside the pancreatic tissue, close to the intestinal wall (a, a″', c, c'). Neurons inside the pancreatic tissue are also visible; however, they were mostly galanin-negative (arrowheads, b). Galanin-IR fibers richly supplied pancreatic islets (c). I intestine, Pi pancreatic islet. Scale bars 100 μm
From: Podlasz P, Jakimiuk A, Chmielewska-Krzesinska M, Kasica N, Nowik N, Kaleczyc J.
Galanin regulates blood glucose level in the zebrafish: a morphological and functional study.
Histochem Cell Biol. 2016 Jan;145(1):105-17.
Rat anti Human Somatostatin
- Product Type
- Monoclonal Antibody
- Clone
- YC7
- Isotype
- IgG2b
- Specificity
- Somatostatin
Antibody Quality and Performance Guarantee
| Rat anti Human somatostatin antibody, clone YC7 recognizes somatostatin, a hormone involved in endocrine system regulation through interactions with pituitary growth hormone, thyroid stimulating hormone, and a number of hormones of the gastrointestinal tract. Somatostatin has active 14 amino acid and 28 amino acid forms, produced by alternate cleavage of the gene product. This antibody does not cross react with enkephalins, other endorphins, substance P, or CGRP. It partially cross reacts with somatostatin fragments. The epitope recognized by this antibody lies within the 1-14 amino acid sequence of cyclic somatostatin. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Guinea Pig Pig Mouse Zebrafish - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Somatostatin conjugated to thyroglobulin.
- Fusion Partners
- Spleen cells from rats immunized with somatostatin conjugated to thyroglobulin were fused with a rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Immunohistology - Paraffin | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for Somatostatin antibody
-
Hens, J. et al. (2000) Mucosal projections of enteric neurons in the porcine small intestine.
J Comp Neurol. 421: 429-36. -
Hens, J. et al. (2001) Morphological and neurochemical identification of enteric neurones with mucosal projections in the human small intestine.
J Neurochem. 76: 464-71. -
Kaleczyc, J. et al. (2002) Distribution, immunohistochemical characteristics and nerve pathways of primary sensory neurons supplying the porcine vas deferens.
Cell Tissue Res. 310: 9-17. -
Van Nassauw, L. et al. (2002) Neurochemical identification of enteric neurons expressing P2X(3) receptors in the guinea-pig ileum.
Histochem Cell Biol. 118 (3): 193-203. -
Geuens, E. et al. (2003) A globin in the nucleus!
J Biol Chem. 278: 30417-20. -
Pidsudko, Z. et al. (2004) Distribution and chemical coding of neurons in intramural ganglia of the porcine urinary bladder trigone.
Folia Histochem Cytobiol. 42: 3-11. -
Kaleczyc, J. et al. (2007) The distribution and chemical coding of intramural neurons supplying the porcine stomach - the study on normal pigs and on animals suffering from swine dysentery.
Anat Histol Embryol. 36 (3): 186-93. -
Pidsudko, Z. et al. (2008) Distribution and chemical coding of intramural neurons in the porcine ileum during proliferative enteropathy.
J Comp Pathol. 138 (1): 23-31.
View The Latest Product References
-
Pidsudko, Z. (2014) Immunohistochemical characteristics and distribution of sensory dorsal root Ganglia neurons supplying the urinary bladder in the male pig.
J Mol Neurosci. 52 (1): 71-81. -
Podlasz, P. et al. (2016) Galanin regulates blood glucose level in the zebrafish: a morphological and functional study.
Histochem Cell Biol. 145 (1): 105-17. -
Ismail, H.I. & Ali, A.M. (2015) Immunohistochemical Studies on The Endocrine Cells in The Thymus of The One-Humped-Camel (Camelus dromedarius)
J Camel Pract Res. 22 (2): 251. -
Zacharko-Siembida, A. et al. (2017) An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig.
Acta Veterinaria. 67 (3): 397-408. -
Palus, K. et al. (2018) Neurochemical characteristics of calbindin-like immunoreactive coeliac-cranial mesenteric ganglion complex (CCMG) neurons supplying the pre-pyloric region of the porcine stomach.
Tissue Cell. 50: 8-14. -
Księżarczyk, M.M. & Arciszewski, M.B. (2021) Immunohistochemical study on the expressions of neuropeptides in the superficial and deep gland of the third eyelid of pigs.
Anat Histol Embryol. 50 (3): 579-87. -
Jana, B. & Całka, J. (2020) Endometritis Changes the Neurochemical Characteristics of the Caudal Mesenteric Ganglion Neurons Supplying the Gilt Uterus.
Animals (Basel). 10 (5): 891.
- RRID
- AB_2195908
- UniProt
- P61278
- Entrez Gene
- SST
- GO Terms
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0007268 synaptic transmission
- GO:0007584 response to nutrient
- GO:0007586 digestion
- GO:0008285 negative regulation of cell proliferation
- GO:0008628 induction of apoptosis by hormones
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