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RUNX1 antibody | EF03/2B4

Mouse anti RUNX1

Product Type
PrecisionAb Monoclonal
Clone
EF03/2B4
Isotype
IgG1
Format
Purified
Specificity
RUNX1

Product Code Applications Pack Size List Price Your Price Qty
VMA00512
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human RUNX1 antibody recognizes RUNX1, also known as AML1-EVI-1 fusion protein, Runt-related transcription factor 1, SL3/AKV core-binding factor alpha B subunit, acute myeloid leukemia 1 protein, oncogene AML-1 or polyomavirus enhancer-binding protein 2 alpha B subunit.

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by RUNX1 gene represents the alpha subunit of CBF and is thought to be involved in the development of normal haematopoiesis. Chromosomal translocations involving RUNX1 are well-documented and have been associated with several types of leukemia (Aydin et al. 2016). Three transcript variants encoding different isoforms have been found for RUNX1 (provided by RefSeq, Jul 2008).

Mouse anti Human RUNX1 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RUNX1 detects a band of approximately 55 kDa in MOLT-4 cell lysate.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli derived recombinant protein corresponding to amino acids 190-453 of human RUNX1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q01196
Entrez Gene
RUNX1

VMA00512

160801

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