RPL6 antibody | AB01/1E12

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Mouse anti RPL6

Product Type
PrecisionAb Monoclonal
Clone
AB01/1E12
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00969
Datasheet
WB
SDS
100 µl loader

Mouse anti RPL6 antibody recognizes 60S ribosomal protein L6, also known as TXREB1. RPL6 is a ribosomal protein which interacts with histone H2A and is recruited to DNA damage sites under the control of PARP. Here, RPL6 participates in the DNA damage response (Yang et al. 2019). RPL6 has been found to be upregulated in multidrug-resistant cancer cells, and could protect gastric cancer from drug-mediated apoptosis. It has been suggested that down-regulation of RPL6 in gastric cancer treatment could suppress cell growth and cell cycle progression (Wu et al. 2011). RPL6 has also been found to promote proliferation and tumorigenesis of gallbladder cancer cells through interaction with NSUN2 (Gao et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti RPL6 detects a band of approximately 33 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-340 of human RPL6
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q02878
Entrez Gene
RPL6
GO Terms
GO:0022625 cytosolic large ribosomal subunit
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0006355 regulation of transcription, DNA-dependent
GO:0006414 translational elongation
GO:0006415 translational termination
GO:0019083 viral transcription
GO:0031018 endocrine pancreas development
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RPL6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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