Prostate Specific Antigen antibody | OTI1A1

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Mouse anti Prostate Specific Antigen

Product Type
PrecisionAb Monoclonal
Clone
OTI1A1
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00275
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human prostate specific antigen antibody recognizes the prostate specific antigen (PSA), also known as P-30 antigen, gamma-seminoprotein, kallikrein-3, semenogelase and seminin.

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. KLK3 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. A product of the KLK3 gene, PSA is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of PSA, in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of KLK3 generates several transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).

Mouse anti Human prostate specific antigen antibody detects a band of 29 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti prostate specific antigen detects a band of approximately 29 kDa in LNCaP cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 25-261 of human prostate specific antigen (NP_001639) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P07288 Related reagents
Entrez Gene
KLK3 Related reagents
GO Terms
GO:0004252 serine-type endopeptidase activity
GO:0005576 extracellular region
GO:0006508 proteolysis
GO:0016525 negative regulation of angiogenesis
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Prostate Specific Antigen antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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