PCNA antibody

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Anti PCNA Antibody (PrecisionAb™ Monoclonal Antibody) thumbnail image 2

Anti PCNA Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PCNA antibody (**VMA00018**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to PCNA (molecular weight 29 kDa).

Anti PCNA Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 2 — Western blot analysis of COS-7 whole cell lysates probed with PCNA antibody (**VMA00018**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to PCNA (molecular weight 29 kDa).

Mouse anti PCNA

PCNA antibody recognizes proliferating cell nuclear antigen, also known as PCNA or cyclin. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00018 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti PCNA antibody recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravo et al. 1987).

PCNA is highly conserved between mammalian species and other vertebrates and this antibody has been shown to detect PCNA in a number of species including rat, mouse and African green monkey

Mouse anti PCNA antibody recognizes proliferating cell nuclear antigen as a single band of ~29 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species

Human

Western Blotting

Anti PCNA antibody detects a band of approximately 29 kDa in HeLa cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
African green monkey
Rat
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Rat PCNA made in the Protein A expression vector pR1T2T

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P12004 Related reagents
Entrez Gene: PCNA Related reagents
GO Terms: GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity; GO:0000722 telomere maintenance via recombination; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0005663 DNA replication factor C complex; GO:0043626 PCNA complex; GO:0006271 DNA strand elongation involved in DNA replication; GO:0006275 regulation of DNA replication; GO:0006283 transcription-coupled nucleotide-excision repair; GO:0006297 nucleotide-excision repair, DNA gap filling; GO:0006298 mismatch repair; GO:0008283 cell proliferation; GO:0015630 microtubule cytoskeleton; GO:0048015 phosphatidylinositol-mediated signaling; GO:0019985 translesion synthesis; GO:0030337 DNA polymerase processivity factor activity; GO:0032077 positive regulation of deoxyribonuclease activity; GO:0032139 dinucleotide insertion or deletion binding; GO:0032201 telomere maintenance via semi-conservative replication; GO:0032405 MutLalpha complex binding; GO:0043596 nuclear replication fork; GO:0070557 PCNA-p21 complex
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of PCNA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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