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PCNA antibody

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Western Blot Validated
Anti PCNA Antibody (PrecisionAb™ Monoclonal Antibody) thumbnail image 1 Anti PCNA Antibody (PrecisionAb™ Monoclonal Antibody) thumbnail image 2
Anti PCNA Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PCNA antibody (VMA00018) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to PCNA (molecular weight 29 kDa).
Anti PCNA Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 2
Western blot analysis of COS-7 whole cell lysates probed with PCNA antibody (VMA00018) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to PCNA (molecular weight 29 kDa).

Mouse anti PCNA

PCNA antibody recognizes proliferating cell nuclear antigen, also known as PCNA or cyclin. The antibody has been extensively validated for western blotting using whole cell lysates.

PCNA Antibody with Control Lysate

PCNA antibody recognizes proliferating cell nuclear antigen, also known as PCNA or cyclin. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Format
Purified
PrecisionAb Antibodies

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00018 WB 100 µl
VMA00018KT WB 2 Westerns
With Lysate 400µg
Mouse anti PCNA antibody recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravo et al. 1987).

PCNA is highly conserved between mammalian species and other vertebrates and this antibody has been shown to detect PCNA in a number of species including rat, mouse and African green monkey

Mouse anti PCNA antibody recognizes proliferating cell nuclear antigen as a single band of ~29 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti PCNA antibody detects a band of approximately 29 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
African green monkey
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Rat PCNA made in the Protein A expression vector pR1T2T
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P12004 Related reagents
Entrez Gene
PCNA Related reagents
GO Terms
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity
GO:0000722 telomere maintenance via recombination
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005663 DNA replication factor C complex
GO:0043626 PCNA complex
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006275 regulation of DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0006298 mismatch repair
GO:0008283 cell proliferation
GO:0015630 microtubule cytoskeleton
GO:0048015 phosphatidylinositol-mediated signaling
GO:0019985 translesion synthesis
GO:0030337 DNA polymerase processivity factor activity
GO:0032077 positive regulation of deoxyribonuclease activity
GO:0032139 dinucleotide insertion or deletion binding
GO:0032201 telomere maintenance via semi-conservative replication
GO:0032405 MutLalpha complex binding
GO:0043596 nuclear replication fork
GO:0070557 PCNA-p21 complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PCNA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
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