Find out more about A.I.den - our SearchBot

PCNA antibody

100% Secure
Western Blot Validated
Anti PCNA Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti PCNA Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti PCNA Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of COS-7 whole cell lysates probed with PCNA antibody (VMA00018) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to PCNA (molecular weight 29 kDa).
Anti PCNA Antibody (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates probed with PCNA antibody (VMA00018) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to PCNA (molecular weight 29 kDa).

Mouse anti PCNA

Product Type
PrecisionAb Monoclonal
Isotype
IgG2a
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00018
Datasheet
WB
SDS
100 µl loader

Mouse anti PCNA antibody recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravo et al. 1987).

PCNA is highly conserved between mammalian species and other vertebrates and this antibody has been shown to detect PCNA in a number of species including rat, mouse and African green monkey

Mouse anti PCNA antibody recognizes proliferating cell nuclear antigen as a single band of ~29 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti PCNA antibody detects a band of approximately 29 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
African green monkey
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Rat PCNA made in the Protein A expression vector pR1T2T

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P12004
Entrez Gene
PCNA
GO Terms
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity
GO:0000722 telomere maintenance via recombination
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005663 DNA replication factor C complex
GO:0043626 PCNA complex
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006275 regulation of DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0006298 mismatch repair
GO:0008283 cell proliferation
GO:0015630 microtubule cytoskeleton
GO:0048015 phosphatidylinositol-mediated signaling
GO:0019985 translesion synthesis
GO:0030337 DNA polymerase processivity factor activity
GO:0032077 positive regulation of deoxyribonuclease activity
GO:0032139 dinucleotide insertion or deletion binding
GO:0032201 telomere maintenance via semi-conservative replication
GO:0032405 MutLalpha complex binding
GO:0043596 nuclear replication fork
GO:0070557 PCNA-p21 complex
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PCNA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
View more products with PCNA specificity