NCK2 antibody | I01/7A10

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Anti NCK2 Antibody, clone I01/7A10 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti NCK2 Antibody, clone I01/7A10 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti NCK2 Antibody, clone I01/7A10 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with NCK2 antibody (1/500, **VMA00908**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to NCK2 (molecular weight 48 kDa).

Anti NCK2 Antibody, clone I01/7A10 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates probed with NCK2 antibody (1/500, **VMA00908**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to NCK2 (molecular weight 48 kDa).

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Mouse anti NCK2

Product Type: PrecisionAb Monoclonal
Clone: I01/7A10
Isotype: IgG1
Format: Purified
Specificity: NCK2

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VMA00908 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti NCK2 antibody recognizes cytoplasmic protein NCK2, also known as GRB4.

NCK2, alongside NCK1, comprise the NCK family of SH2/SH3-containing adaptor proteins. These proteins couple activated membrane receptors to specific downstream effectors, with a range of biological effects. Both proteins can regulate cell adhesion and migration via tyrosine phosphorylation and cytoskeletal remodeling (Morris et al. 2017). Despite their similarity, NCK1 and NCK2 appear to have distinct roles (Labelle-Côté et al. 2011). For example, NCK2 but not NCK1 appears to be involved with specific processes of axon trajectory during limb development (Chang et al. 2018). NCK2, but not NCK1, also appears to be a positive regulator of stimulation of the Erk pathway via the T cell antigen receptor during the activation of T lymphocytes (Ngoenkam et al. 2014). In cancer, NCK2 overexpression has been identified in human metastatic melanoma compared with non-metastatic melanoma (Labelle-Côté et al. 2011). NCK has been identified as a driver of breast cancer progression and metastasis, and has been suggested as a therapeutic target in aggressive cancers (Morris et al. 2017).

Target Species: Human
Western Blotting: Mouse anti NCK2 antibody detects a band of approximately 48 kDa in THP-1 cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: E. coli-derived recombinant protein, aa 1-306 of human NCK2
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: As supplied, 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20^oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: O43639
Entrez Gene: NCK2
GO Terms: GO:0005829 cytosol; GO:0005783 endoplasmic reticulum; GO:0006417 regulation of translation; GO:0007172 signal complex assembly; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007176 regulation of epidermal growth factor receptor activity; GO:0007411 axon guidance; GO:0008093 cytoskeletal adaptor activity; GO:0008285 negative regulation of cell proliferation; View More GO Terms; GO:0030159 receptor signaling complex scaffold activity; GO:0030838 positive regulation of actin filament polymerization; GO:0042102 positive regulation of T cell proliferation; GO:0042110 T cell activation