NCK2 antibody | I01/7A10
Mouse anti NCK2 antibody recognizes cytoplasmic protein NCK2, also known as GRB4.
NCK2, alongside NCK1, comprise the NCK family of SH2/SH3-containing adaptor proteins. These proteins couple activated membrane receptors to specific downstream effectors, with a range of biological effects. Both proteins can regulate cell adhesion and migration via tyrosine phosphorylation and cytoskeletal remodeling (Morris et al. 2017). Despite their similarity, NCK1 and NCK2 appear to have distinct roles (Labelle-Côté et al. 2011). For example, NCK2 but not NCK1 appears to be involved with specific processes of axon trajectory during limb development (Chang et al. 2018). NCK2, but not NCK1, also appears to be a positive regulator of stimulation of the Erk pathway via the T cell antigen receptor during the activation of T lymphocytes (Ngoenkam et al. 2014). In cancer, NCK2 overexpression has been identified in human metastatic melanoma compared with non-metastatic melanoma (Labelle-Côté et al. 2011). NCK has been identified as a driver of breast cancer progression and metastasis, and has been suggested as a therapeutic target in aggressive cancers (Morris et al. 2017).
NCK2, alongside NCK1, comprise the NCK family of SH2/SH3-containing adaptor proteins. These proteins couple activated membrane receptors to specific downstream effectors, with a range of biological effects. Both proteins can regulate cell adhesion and migration via tyrosine phosphorylation and cytoskeletal remodeling (Morris et al. 2017). Despite their similarity, NCK1 and NCK2 appear to have distinct roles (Labelle-Côté et al. 2011). For example, NCK2 but not NCK1 appears to be involved with specific processes of axon trajectory during limb development (Chang et al. 2018). NCK2, but not NCK1, also appears to be a positive regulator of stimulation of the Erk pathway via the T cell antigen receptor during the activation of T lymphocytes (Ngoenkam et al. 2014). In cancer, NCK2 overexpression has been identified in human metastatic melanoma compared with non-metastatic melanoma (Labelle-Côté et al. 2011). NCK has been identified as a driver of breast cancer progression and metastasis, and has been suggested as a therapeutic target in aggressive cancers (Morris et al. 2017).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti NCK2 antibody detects a band of approximately 48 kDa in THP-1 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant protein, aa 1-306 of human NCK2
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- O43639
- Entrez Gene
- NCK2
- GO Terms
- GO:0005829 cytosol
- GO:0005783 endoplasmic reticulum
- GO:0006417 regulation of translation
- GO:0007172 signal complex assembly
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007176 regulation of epidermal growth factor receptor activity
- GO:0007411 axon guidance
- GO:0008093 cytoskeletal adaptor activity
- GO:0008285 negative regulation of cell proliferation
- GO:0030159 receptor signaling complex scaffold activity
- GO:0030838 positive regulation of actin filament polymerization
- GO:0042102 positive regulation of T cell proliferation
- GO:0042110 T cell activation
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of NCK2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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