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MUCIN 1 antibody | MUC1/520

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Western Blot Validated
Anti MUCIN 1 Antibody, clone MUC1/520 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti MUCIN 1 Antibody, clone MUC1/520 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti MUCIN 1 Antibody, clone MUC1/520 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti MUCIN 1 Antibody, clone MUC1/520 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with mucin 1 antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 14 second exposure. Arrow points to mucin 1 (molecular weight 400 kDa).
Anti MUCIN 1 Antibody, clone MUC1/520 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of mucin 1 CRISPR knockout HeLa (MUCIN 1 KO) and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti mucin 1 antibody (VMA00660) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 3 second and (B) 30 second exposure.
Anti MUCIN 1 Antibody, clone MUC1/520 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of mucin 1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Mouse anti MUCIN 1

Product Type
PrecisionAb Monoclonal
Clone
MUC1/520
Isotype
IgG2a
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00660
Datasheet
WB
SDS
100 µl loader

Product Details

Target Species
Human
Western Blotting
Mouse anti mucin 1 antibody detects a band of approximately 400 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P15941
Entrez Gene
MUC1
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of MUCIN 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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