mTOR antibody | RM274
Rabbit anti Human mTOR antibody recognizes mammalian/mechanistic target of rapamycin (mTOR), also known as FRAP, RAFT1. mTOR is a serine/threonine protein kinase belonging to the PI3K-related kinse (PIKK) family. mTOR forms two distinct multiprotein complexes named mTORC1 and mTORC2, which play key roles in regulatory processes of cells (Xie et al. 2016). mTOR regulates cell growth and metabolism in response to environmental cues and cell stress, including nutrient starvation, growth factor deprivation and hypoxia (Lamm et al. 2019). The mTOR signaling pathway plays a role in cancer cell proliferation, cell cycle and apoptosis. mTOR dysregulation is linked to tumorigenesis, and is associated with a poor prognosis (Wang et al. 2018). mTORC1 hyperactivation is estimated to occur in up to 70% of human tumors, and mTOR has been suggested as a potential target for anti-cancer therapy.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A peptide corresponding to Human serine/threonine-protein kinase mTOR
Storage Information
- Storage
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P42345
- Entrez Gene
- MTOR
- GO Terms
- GO:0000139 Golgi membrane
- GO:0016049 cell growth
- GO:0005764 lysosome
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005741 mitochondrial outer membrane
- GO:0005789 endoplasmic reticulum membrane
- GO:0005942 phosphatidylinositol 3-kinase complex
- GO:0007584 response to nutrient
- GO:0008286 insulin receptor signaling pathway
- GO:0018105 peptidyl-serine phosphorylation
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030163 protein catabolic process
- GO:0031295 T cell costimulation
- GO:0031929 TOR signaling cascade
- GO:0031931 TORC1 complex
- GO:0031932 TORC2 complex
- GO:0043200 response to amino acid stimulus
- GO:0046777 protein autophosphorylation
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051219 phosphoprotein binding
- Regulatory
- For research purposes only
Applications of mTOR antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/500 | 1/1000 | |
| Western Blotting | 1/1000 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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