mTOR antibody | RM274

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Rabbit anti mTOR

Product Type
Monoclonal Antibody
Clone
RM274
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6317
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human mTOR antibody recognizes mammalian/mechanistic target of rapamycin (mTOR), also known as FRAP, RAFT1. mTOR is a serine/threonine protein kinase belonging to the PI3K-related kinse (PIKK) family. mTOR forms two distinct multiprotein complexes named mTORC1 and mTORC2, which play key roles in regulatory processes of cells (Xie et al. 2016). mTOR regulates cell growth and metabolism in response to environmental cues and cell stress, including nutrient starvation, growth factor deprivation and hypoxia (Lamm et al. 2019). The mTOR signaling pathway plays a role in cancer cell proliferation, cell cycle and apoptosis. mTOR dysregulation is linked to tumorigenesis, and is associated with a poor prognosis (Wang et al. 2018). mTORC1 hyperactivation is estimated to occur in up to 70% of human tumors, and mTOR has been suggested as a potential target for anti-cancer therapy.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to Human serine/threonine-protein kinase mTOR

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
P42345
Entrez Gene
MTOR
GO Terms
GO:0000139 Golgi membrane
GO:0016049 cell growth
GO:0005764 lysosome
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005741 mitochondrial outer membrane
GO:0005789 endoplasmic reticulum membrane
GO:0005942 phosphatidylinositol 3-kinase complex
GO:0007584 response to nutrient
GO:0008286 insulin receptor signaling pathway
GO:0018105 peptidyl-serine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030163 protein catabolic process
GO:0031295 T cell costimulation
GO:0031929 TOR signaling cascade
GO:0031931 TORC1 complex
GO:0031932 TORC2 complex
GO:0043200 response to amino acid stimulus
GO:0046777 protein autophosphorylation
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051219 phosphoprotein binding
Regulatory
For research purposes only

Applications of mTOR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/500 1/1000
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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