ISG15 antibody | AB01/4E8

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Anti ISG15 Antibody, clone AB01/4E8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti ISG15 Antibody, clone AB01/4E8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ISG15 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, STAR207P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to ISG15 (molecular weight 16 kDa)

Mouse anti ISG15

Product Type: PrecisionAb Monoclonal
Clone: AB01/4E8
Isotype: IgG2b
Format: Purified
Specificity: ISG15

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VMA00778 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human ISG15 antibody recognizes the ubiquitin-like protein ISG15, also known as interferon-alpha-inducible protein (clone IFI-15K), interferon-induced 17-kDa/15-kDa protein and ubiquitin cross-reactive protein.

The protein encoded by ISG15 is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted (provided by RefSeq, Dec 2012).

Mouse anti Human ISG15 antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti ISG15 detects a band of approximately 16 kDa in MCF7 cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-165 of human ISG15
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P05161
Entrez Gene: ISG15
GO Terms: GO:0005515 protein binding; GO:0009615 response to virus; GO:0005829 cytosol; GO:0005615 extracellular space; GO:0007267 cell-cell signaling; GO:0032020 ISG15-protein conjugation; GO:0032480 negative regulation of type I interferon production; GO:0044419 interspecies interaction between organisms; GO:0060337 type I interferon-mediated signaling pathway; View More GO Terms