ISG15 antibody | AB01/4E8

Mouse anti ISG15

Product Type
PrecisionAb Monoclonal
Clone
AB01/4E8
Isotype
IgG2b
Format
Purified
Specificity
ISG15

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VMA00778
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SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human ISG15 antibody recognizes the ubiquitin-like protein ISG15, also known as interferon-alpha-inducible protein (clone IFI-15K), interferon-induced 17-kDa/15-kDa protein and ubiquitin cross-reactive protein.

The protein encoded by ISG15 is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted (provided by RefSeq, Dec 2012).

Mouse anti Human ISG15 antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti ISG15 detects a band of approximately 16 kDa in MCF7 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-165 of human ISG15
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P05161
Entrez Gene
ISG15
GO Terms
GO:0005515 protein binding
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005615 extracellular space
GO:0007267 cell-cell signaling
GO:0032020 ISG15-protein conjugation
GO:0032480 negative regulation of type I interferon production
GO:0044419 interspecies interaction between organisms
GO:0060337 type I interferon-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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