ISG15 antibody | AB01/4E8
Mouse anti Human ISG15 antibody recognizes the ubiquitin-like protein ISG15, also known as interferon-alpha-inducible protein (clone IFI-15K), interferon-induced 17-kDa/15-kDa protein and ubiquitin cross-reactive protein.
The protein encoded by ISG15 is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted (provided by RefSeq, Dec 2012).
Mouse anti Human ISG15 antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by ISG15 is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted (provided by RefSeq, Dec 2012).
Mouse anti Human ISG15 antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti ISG15 detects a band of approximately 16 kDa in MCF7 cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-165 of human ISG15
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P05161
- Entrez Gene
- ISG15
- GO Terms
- GO:0005515 protein binding
- GO:0009615 response to virus
- GO:0005829 cytosol
- GO:0005615 extracellular space
- GO:0007267 cell-cell signaling
- GO:0032020 ISG15-protein conjugation
- GO:0032480 negative regulation of type I interferon production
- GO:0044419 interspecies interaction between organisms
- GO:0060337 type I interferon-mediated signaling pathway
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ISG15 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
