IL-1 Beta antibody | OTI1A7

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Anti Interleukin-1 beta Antibody, clone OTI1A7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with IL-1 Beta antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 18 second exposure. Arrow points to IL-1 Beta (molecular weight 31 kDa)

Mouse anti Interleukin-1 beta

Product Type: PrecisionAb Monoclonal
Clone: OTI1A7
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00633 Datasheet	WB SDS	100 µl	Log in		Get Quote

Mouse anti Human interleukin-1 beta antibody recognizes interleukin-1 beta, also known as catabolin, pre-interleukin 1 beta and pro-interleukin-1-beta.

The protein encoded by IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (provided by RefSeq, Jul 2008).

Mouse anti Human interleukin-1 beta antibody detects a band of 31 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Mouse anti interleukin-1 beta antibody detects a band of approximately 31 kDa in LPS-stimulated THP-1 cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen: HEK293-derived full length recombinant IL-1 beta

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Guarantee: 12 months from date of despatch

More Information

UniProt: P01584
Entrez Gene: IL1B
GO Terms: GO:0000187 activation of MAPK activity; GO:0001660 fever generation; GO:0001934 positive regulation of protein phosphorylation; GO:0002711 positive regulation of T cell mediated immunity; GO:0006915 apoptosis; GO:0005829 cytosol; GO:0005125 cytokine activity; GO:0005149 interleukin-1 receptor binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0007267 cell-cell signaling; GO:0007566 embryo implantation; GO:0050796 regulation of insulin secretion; GO:0008083 growth factor activity; GO:0008285 negative regulation of cell proliferation; GO:0010575 positive regulation vascular endothelial growth factor production; GO:0010829 negative regulation of glucose transport; GO:0014805 smooth muscle adaptation; GO:0019221 cytokine-mediated signaling pathway; GO:0019904 protein domain specific binding; GO:0030730 sequestering of triglyceride; GO:0031622 positive regulation of fever generation; GO:0032308 positive regulation of prostaglandin secretion; GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production; GO:0032729 positive regulation of interferon-gamma production; GO:0032755 positive regulation of interleukin-6 production; GO:0034116 positive regulation of heterotypic cell-cell adhesion; GO:0035505 positive regulation of myosin light chain kinase activity; GO:0042102 positive regulation of T cell proliferation; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0043407 negative regulation of MAP kinase activity; GO:0045086 positive regulation of interleukin-2 biosynthetic process; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045766 positive regulation of angiogenesis; GO:0045840 positive regulation of mitosis; GO:0046627 negative regulation of insulin receptor signaling pathway; GO:0046827 positive regulation of protein export from nucleus; GO:0050995 negative regulation of lipid catabolic process; GO:0050996 positive regulation of lipid catabolic process; GO:0051044 positive regulation of membrane protein ectodomain proteolysis; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051781 positive regulation of cell division; GO:0060355 positive regulation of cell adhesion molecule production; GO:0060559 positive regulation of calcidiol 1-monooxygenase activity; GO:0070164 negative regulation of adiponectin secretion
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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