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IL-1 Beta antibody | OTI1A7

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Western Blot Validated
Anti Interleukin-1 beta Antibody, clone OTI1A7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with IL-1 Beta antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 18 second exposure. Arrow points to IL-1 Beta (molecular weight 31 kDa)

Mouse anti Interleukin-1 beta

Product Type
PrecisionAb Monoclonal
Clone
OTI1A7
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00633
Datasheet
WB
SDS
100 µl loader

Mouse anti Human interleukin-1 beta antibody recognizes interleukin-1 beta, also known as catabolin, pre-interleukin 1 beta and pro-interleukin-1-beta.

The protein encoded by IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (provided by RefSeq, Jul 2008).

Mouse anti Human interleukin-1 beta antibody detects a band of 31 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti interleukin-1 beta antibody detects a band of approximately 31 kDa in LPS-stimulated THP-1 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
HEK293-derived full length recombinant IL-1 beta

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P01584
Entrez Gene
IL1B
GO Terms
GO:0000187 activation of MAPK activity
GO:0001660 fever generation
GO:0001934 positive regulation of protein phosphorylation
GO:0002711 positive regulation of T cell mediated immunity
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0007267 cell-cell signaling
GO:0007566 embryo implantation
GO:0050796 regulation of insulin secretion
GO:0008083 growth factor activity
GO:0008285 negative regulation of cell proliferation
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010829 negative regulation of glucose transport
GO:0014805 smooth muscle adaptation
GO:0019221 cytokine-mediated signaling pathway
GO:0019904 protein domain specific binding
GO:0030730 sequestering of triglyceride
GO:0031622 positive regulation of fever generation
GO:0032308 positive regulation of prostaglandin secretion
GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
GO:0032729 positive regulation of interferon-gamma production
GO:0032755 positive regulation of interleukin-6 production
GO:0034116 positive regulation of heterotypic cell-cell adhesion
GO:0035505 positive regulation of myosin light chain kinase activity
GO:0042102 positive regulation of T cell proliferation
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0043407 negative regulation of MAP kinase activity
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045766 positive regulation of angiogenesis
GO:0045840 positive regulation of mitosis
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0046827 positive regulation of protein export from nucleus
GO:0050995 negative regulation of lipid catabolic process
GO:0050996 positive regulation of lipid catabolic process
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051781 positive regulation of cell division
GO:0060355 positive regulation of cell adhesion molecule production
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0070164 negative regulation of adiponectin secretion
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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