IL-1 Beta antibody | OTI1A7
The protein encoded by IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (provided by RefSeq, Jul 2008).
Mouse anti Human interleukin-1 beta antibody detects a band of 31 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti interleukin-1 beta antibody detects a band of approximately 31 kDa in LPS-stimulated THP-1 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Immunogen
- HEK293-derived full length recombinant IL-1 beta
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01584
- Entrez Gene
- IL1B
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0001660 fever generation
- GO:0001934 positive regulation of protein phosphorylation
- GO:0002711 positive regulation of T cell mediated immunity
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0005125 cytokine activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0007267 cell-cell signaling
- GO:0007566 embryo implantation
- GO:0050796 regulation of insulin secretion
- GO:0008083 growth factor activity
- GO:0008285 negative regulation of cell proliferation
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010829 negative regulation of glucose transport
- GO:0014805 smooth muscle adaptation
- GO:0019221 cytokine-mediated signaling pathway
- GO:0019904 protein domain specific binding
- GO:0030730 sequestering of triglyceride
- GO:0031622 positive regulation of fever generation
- GO:0032308 positive regulation of prostaglandin secretion
- GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0034116 positive regulation of heterotypic cell-cell adhesion
- GO:0035505 positive regulation of myosin light chain kinase activity
- GO:0042102 positive regulation of T cell proliferation
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0043407 negative regulation of MAP kinase activity
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045766 positive regulation of angiogenesis
- GO:0045840 positive regulation of mitosis
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0046827 positive regulation of protein export from nucleus
- GO:0050995 negative regulation of lipid catabolic process
- GO:0050996 positive regulation of lipid catabolic process
- GO:0051044 positive regulation of membrane protein ectodomain proteolysis
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051781 positive regulation of cell division
- GO:0060355 positive regulation of cell adhesion molecule production
- GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
- GO:0070164 negative regulation of adiponectin secretion
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of IL-1 Beta antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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