IgM antibody | M15/8

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Mouse anti Human IgM:FITC

Product Type: Monoclonal Antibody
Clone: M15/8
Isotype: IgG1
Specificity: IgM

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References (5)
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Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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MCA1662F Datasheet SDS SDS	F	0.1 mg		Log in
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Anti Human IgM Antibody, clone M15/8 thumbnail image 1

Anti Human IgM Antibody, clone M15/8 thumbnail image 2

Anti Human IgM Antibody, clone M15/8 thumbnail image 3

Anti Human IgM Antibody, clone M15/8 gallery image 1 — **Published customer image:**
Mouse anti Human IgM antibody, clone M15.8 (**MCA1662**) used for the demonstration of IgM expressing infected erythrocytes by immunofluorescence
**Image caption:**
Polyclonal antibodies to PfEMP1 recognize IgM-positive IEs.
HB3R+ live IEs were stained with a mixture of mouse mAb anti-human IgM (1/500 dilution) and rabbit polyclonal HB3var6 NTS-DBLα antibodies (20 μg/ml) (left column) or a mixture of mouse IgG isotype control and non-immunized rabbit IgG control (right column). Secondary incubation was with a mixture of Alexa 488 conjugated anti-rabbit IgG (1/1000) and Alexa 594-conjugated anti-mouse IgG (1/1000). IEs were stained with DAPI (1 μg/ml; scale bar 10 μm). The PfEMP1 antibodies (left column, bottom panel) stained IEs that were also positive for human IgM (left column, middle panel). Camera exposure settings were identical for PfEMP1 antibodies and controls except for human IgM/PfEMP1 with Alexa Fluor 488 which was taken at a shorter exposure setting (20 msecs) than the control (200 msecs), due to the brightness of the signal.

From: Ghumra A, Semblat J-P, Ataide R, Kifude C, Adams Y, Claessens A, *et al*. (2012)
Induction of Strain-Transcending Antibodies Against Group A PfEMP1 Surface Antigens from Virulent Malaria Parasites.
**PLoS Pathog 8(4): e1002665**.
doi: 10.1371/journal.ppat.1002665.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Human IgM Antibody, clone M15/8 gallery image 2 — **Published customer image:**
Mouse anti Human IgM antibody, clone M15.8 (**MCA1662**) used for the demonstration of IgM expressing infected erythrocytes by immunofluorescence.
**Image caption:**
Homologous and heterologous polyclonal antibodies to PfEMP1 recognize IgM-positive IEs. a) Parasite strain TM284R+ was stained in a live cell IFA with a mixture of rabbit polylconal antibodies to PfEMP1 (homologous or heterologous) at 20 μg/ml and mouse anti-human IgM mAb (**Serotec MCA1662 1/500 dilution**). Secondary incubation was with a mixture of Alexa Fluor 488 conjugated anti-rabbit IgG (1/1000) and Alexa Fluor 594-conjugated anti-mouse IgG (1/1000). IEs were stained with DAPI (1 µg/ml; scale bar 10 μm). IgM-positive IEs (right column) show punctate/rim surface fluorescence with both homologous antibodies (anti-TM284var1, 2nd row, middle column) and heterologous antibodies (anti-ITvar60, 3rd row, middle column). 94–100% of the PfEMP1 antibody-positive cells were IgM-positive, and 100% of the IgM-positive cells were PfEMP1-antibody positive. At this concentration TM180var1 antibodies give very faint punctate fluorescence on IgM-positive IEs (5th row, middle column) whereas HB3var6 antibodies are negative (4th row, middle column). At higher concentrations (100–400 μg/ml) both TM180var1 and HB3var6 antibodies stain IgM-positive IEs. b) Specificity controls (with parasite strain TM284R+ as above) show that the Alexa 488 conjugated anti-rabbit IgG secondary does not recognize the mouse anti-human IgM mAb (top row) and that the Alexa 594-conjugated anti-mouse IgG does not recognize the rabbit polyclonal antibodies (bottom row). Camera exposure settings and image handling were identical for PfEMP1 antibodies and controls.

From: Ghumra A, Semblat J-P, Ataide R, Kifude C, Adams Y, Claessens A, *et al*. (2012)
Induction of Strain-Transcending Antibodies Against Group A PfEMP1 Surface Antigens from Virulent Malaria Parasites.
**PLoS Pathog 8(4): e1002665**.
doi: 10.1371/journal.ppat.1002665.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Human IgM Antibody, clone M15/8 gallery image 3 — Sandwich ELISA analysis of IgM binding using Mouse anti Human IgM antibody, clone M15/8 (**MCA1662**) as a capture reagent and biotinylated Goat anti Human IgM (**AHP1764**) as a detection regent with purified human IgM (**5275-5504**) as antigen for the generation of the standard curve. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (**Bio-Rad Catalog 168-1135**). Serum samples at 1:10k (green) and 1:100k (blue), similarly plasma samples 1:10k (orange) and 1:100k (red) dilutions are shown.

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IF E

Mouse anti Human IgM monoclonal antibody, clone M15/8 recognizes human IgM, binding to the μ heavy chain. The antibody does not cross-react with other immunoglobulin classes.

Target Species: Human
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
1% bovine serum albumin
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

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References for IgM antibody

Cragg, M.S. et al. (1999) Analysis of the interaction of monoclonal antibodies with surface IgM on neoplastic B-cells.
Br J Cancer. 79 (5-6): 850-7.
Irie, E. et al. (2010) Severe hypogammaglobulinemia persisting for 6 years after treatment with rituximab combined chemotherapy due to arrest of B lymphocyte differentiation together with alteration of T lymphocyte homeostasis.
Int J Hematol. 91: 501-8.
Creasey, A.M. et al. (2003) Nonspecific immunoglobulin M binding and chondroitin sulfate A binding are linked phenotypes of Plasmodium falciparum isolates implicated in malaria during pregnancy.
Infect Immun. 71: 4767-71.
Ghumra, A. et al. (2012) Induction of strain-transcending antibodies against Group A PfEMP1 surface antigens from virulent malaria parasites.
PLoS Pathog. 8 (4): e1002665.
Semblat, J.P. et al. (2015) Identification of the minimal binding region of a Plasmodium falciparum IgM binding PfEMP1 domain.
Mol Biochem Parasitol. 201 (1): 76-82.

Immunofluorescence

RRID: AB_321862
UniProt: P01871
Entrez Gene: IGHM
GO Terms: GO:0003823 antigen binding; GO:0016021 integral to membrane; GO:0005624 membrane fraction