IgG1 antibody | AbD27686

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Human anti Human IgG1 (Fc):HRP

Anti-Human IgG1 Fc Antibody recognizes human IgG1 and can be used for the detection of therapeutic antibodies in human IgG1 format in samples obtained from cynomolgus macaques.

Human anti Human IgG1 (Fc)

Anti-Human IgG1 Fc Antibody recognizes human IgG1 and can be used for the detection of therapeutic antibodies in human IgG1 format in samples obtained from cynomolgus macaques.

Product Type
Monoclonal Antibody
Clone
AbD27686
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA285P E 0.1 mg
HCA285 E 0.1 mg
Human Anti-Human IgG1 Fc Antibody clone AbD27686 is a recombinant monovalent Fab antibody, that recognizes human IgG1 Fc, with an affinity of 76 nM. It has been shown to have a greatly reduced affinity (89-fold) for IgG from cynomolgus macaque compared to human IgG1. It also has strongly reduced binding to rhesus macaque IgG. It does not recognize other human immunoglobulin isotypes (IgG2, IgG3, IgG4, IgA, IgE, IgM) or mouse IgG2a.

This antibody has been generated by Bio-Rad using the HuCAL® antibody library and CysDisplay® technology. It is sold for research use, with implicit permission for use for the detection of therapeutic antibodies from samples obtained from experimental animals. When tested by Bio-Rad in an antigen capture pharmacokinetic assay format, this antibody performs similarly to Mouse Anti-Human IgG (CH2 Domain) Antibody clone R10Z8E9 (MCA5748G) in 10% cynomolgus macaque serum. It recognizes a different epitope on human IgG1 Fc than clone R10Z8E9.

View a summary of anti-human IgG Fc specific antibodies for the detection of therapeutic antibodies.

Product Details

Target Species
Human
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL PLATINUM® phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to horseradish peroxidase (HRP) - liquid
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL PLATINUM® phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Recombinant human IgG1 kappa
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=76 nM by real time, label free molecular interaction analysis on immobilized hIgG1 kappa
Approx. Protein Concentrations
Antibody concentration 0.1 mg/ml
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad
Regulatory
For research purposes only

Applications of IgG1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
Hispec Assay Diluent BUF049A 50 ml E IY
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB