hnRNP M antibody | OTI3F3

Mouse anti hnRNP M

Product Type
PrecisionAb Monoclonal
Clone
OTI3F3
Isotype
IgG2b
Format
Purified
Specificity
hnRNP M

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VMA00260
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Mouse anti Human hnRNP M antibody recognizes heterogeneous nuclear ribonucleoprotein M (hnRNP M), also known as CEA receptor, N-acetylglucosamine receptor 1, heterogenous nuclear ribonucleoprotein M4 and hnRNA-binding protein M4.

Encoded by the HNRNPM gene, hnRNP M belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Heterogeneous nuclear ribonucleoprotein M has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants (provided by RefSeq, Aug 2011).

Mouse anti Human hnRNP M antibody detects a band of 77 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti hnRNP M detects a band of approximately 77 kDa in MOLT-4 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol.
Immunogen
Recombinant protein corresponding to amino acids 1-261 of human hnRNP M (NP_112480) produced in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P52272
Entrez Gene
HNRNPM
GO Terms
GO:0000166 nucleotide binding
GO:0000380 alternative nuclear mRNA splicing, via spliceosome
GO:0003723 RNA binding
GO:0005887 integral to plasma membrane
GO:0005681 spliceosomal complex
GO:0005730 nucleolus
GO:0016363 nuclear matrix
GO:0019904 protein domain specific binding
GO:0042382 paraspeckles
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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