HLA DR antibody | YE2/36-HLK
Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometry
Image caption:
BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP− population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody.
From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009)
The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation.
PLoS Pathog 5(1): e1000255.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometry
Image caption:
BILF1 downregulates surface MHC class I expression and inhibits the T cell recognition of endogenous EBV antigen in LCLs. (A) LCLs were transduced with PLZRS-HABILF1-IRES-GFP retrovirus. After 6 days, surface MHC class I was stained with PE-conjugated W6/32 mAb and MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyze staining in the untransduced, GFP−, population, shown as the solid line histogram, and in the transduced GFP+ (BILF1+) population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody. (B) LCL cultures transduced with control retrovirus or with the BILF1 retrovirus were sorted by flow cytometry to generate GFP+/BILF1− and GFP+/BILF1+ lines to use as targets in assays with EBV-specific T cells. The control and BILF1+ LCL targets were incubated with HLA-matched CD8+ effector T cells clones specific for EBNA1 (HPV), EBNA3A (YPL), or LMP2A (CLG) peptides, or a CD4+ effector T cell clone specific for a BHRF1 (PYY) peptide. After 18 hrs the supernatants were tested for the release of IFN-γ as a measure of T cell recognition. All results are expressed as IFN-γ release in pg/ml and error bars indicate standard deviation of triplicate cultures.
From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009)
The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation.
PLoS Pathog 5(1): e1000255.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometry
Image caption:
HLA-DR positive monocytes. The percentage HLA-DR positive monocytes was decreased in all patients compared to controls (P = 0.002). The pre-operative ("black square") lowest percentage was seen in patients who developed respiratory failure (P = 0.002). Eighteen hours after intramedullary nailing ("open triangle"), a further decrease in HLA-DR positive monocytes was seen in patients with isolated femur fracture (P < 0.001) and multitrauma patients (P = 0.047).
From: Hietbrink, Falco, Leo Koenderman, and Luke PH Leenen.
“Intramedullary Nailing of the Femur and the Systemic Activation of Monocytes and Neutrophils.
World Journal of Emergency Surgery : WJES 6 (2011): 34. PMC. Web. 22 Jan. 2015.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human HLA DR
- Product Type
- Monoclonal Antibody
- Clone
- YE2/36-HLK
- Isotype
- IgG2a
- Specificity
- HLA DR
Antibody Quality and Performance Guarantee
| Rat anti Human HLA DR antibody, clone YE2/36-HLK recognizes a monomorphic determinant of human HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR. Rat anti Human HLA DR antibody, clone YE2/36-HLK has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB (Brickell et al. 1981). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- EHR-B Ramos cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised AO rats were fuzed with cells of the rat Y3 Ag1.2.3 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 1 | |
- 1PLP fixation is recommended for optimal results, see Whiteland et al. for details
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Source Reference
-
Brickell, P.M. et al. (1981) A monoclonal antibody to the HLA-DR product recognizes a polymorphic Ia determinant in mice.
Immunology. 43: 493-501.
References for HLA DR antibody
-
Cruchley, A.T. et al. (1987) Dual expression of the cell-surface antigens HLA-DR and CD1 (T6) by Langerhans cells in human buccal mucosa and skin.
Arch Oral Biol. 32: 849-53. -
Thornhill, M.H. et al. (1989) Enhanced adhesion of autologous lymphocytes to gamma-interferon-treated human endothelial cells in vitro.
Br J Exp Pathol. 70: 59-64. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Becker, D. et al. (1997) Flow-cytometric screening for the modulation of receptor-mediated endocytosis in human dendritic cells: implications for the development of an in vitro technique for predictive testing of contact sensitizers.
J Immunol Methods. 203: 171-80. -
Jonuleit, H. et al. (2000) Induction of interleukin 10-producing, nonproliferating CD4(+) T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells.
J Exp Med. 192: 1213-22. -
Walter, W. et al. (2001) H2-Mbeta 1 and H2-Mbeta 2 heterodimers equally promote clip removal in I-A(q) molecules from autoimmune-prone DBA/1 mice.
J Biol Chem. 276: 11086-91. -
Shimada, A. et al. (2006) Translocation pathway of the intratracheally instilled ultrafine particles from the lung into the blood circulation in the mouse.
Toxicol Pathol. 34: 949-57. -
Colić, M. et al. (2006) Correlation between phenotypic characteristics of mononuclear cells isolated from human periapical lesions and their in vitro production of Th1 and Th2 cytokines.
Arch Oral Biol. 51: 1120-30.
View The Latest Product References
-
Zuo, J. et al. (2009) The Epstein-Barr virus G-protein-coupled receptor contributes to immune evasion by targeting MHC class I molecules for degradation.
PLoS Pathog. 5(1): e1000255. -
Hietbrink, F. et al. (2011) Intramedullary nailing of the femur and the systemic activation of monocytes and neutrophils
World J Emerg Surg. 6: 34. -
Nguyen Hoang, A.T. et al. (2012) Dendritic cell functional properties in a three-dimensional tissue model of human lung mucosa.
Am J Physiol Lung Cell Mol Physiol. 302 (2): L226-37. -
Klotz, B. et al. (2012) 1,25-Dihydroxyvitamin D3 Treatment Delays Cellular Aging in Human Mesenchymal Stem Cells while Maintaining Their Multipotent Capacity.
PLoS One. 7: e29959. -
Thorne, A. et al. (2015) Tumor necrosis factor-α promotes survival and phenotypic maturation of poly(I:C)-treated dendritic cells but impairs their Th1 and Th17 polarizing capability.
Cytotherapy. 17 (5): 633-46. -
Hietbrink F et al. (2015) The Impact of Intramedullary Nailing of Tibia Fractures on the Innate Immune System.
Shock. 44 (3): 209-14. -
GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
PLoS One. 13 (2): e0192244. -
Tripathy, N.K. et al. (2018) Cardiomyogenic Heterogeneity of Clonal Subpopulations of Human Bone Marrow Mesenchymal Stem Cells.
J Stem Cells Regen Med. 14 (1): 27-33.
- RRID
- AB_323671
MCA71R
165903
1806
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