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HLA DP DQ DR antibody | WR18

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Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 1 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 2 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 3 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 4 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 5 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 6 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 7 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 8 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 9 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 10 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 11 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 12 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 13 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 14 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 15 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 16 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 17 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 18 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 19 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 20 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 21 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 22 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 23 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 24 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 25 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 26 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 27 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 28 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 29 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 30 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 31 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 32 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 33 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 34 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 35 Anti Human HLA DP DQ DR Antibody, clone WR18 thumbnail image 36
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 1
Figure A. PE conjugated Mouse anti Human CD11c (MCA2087PE) and Amethyst Orange conjugated Mouse IgG2a isotype control (MCA929AMO). Figure B. PE conjugated Mouse anti Human CD11c (MCA2087PE) and Amethyst Orange conjugated Mouse anti Human HLA DP DQ DR (MCA477AMO). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 2
Figure A. RPE conjugated Mouse anti Human CD11c (MCA2087PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Human CD11c (MCA2087PE) and FITC conjugated Mouse anti Human HLA DP/DQ/DR (MCA477F). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 3
Figure A. FITC conjugated Mouse anti Human CD86 (MCA1118F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE)> Figure B. FITC conjugated Mouse anti Human CD86 (MCA1118F) and RPE conjugated Mouse anti Human HLA DP/DQ/DR (MCA477PE). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 4
Published customer image:
Mouse anti Human HLA DP DQ DR antibody, clone WR18 used for the evaluation of MHC class II expression on dendritic cells by flow cytometry.
Image caption:
Time course of DC differentiation from H1 hESC. Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (a) Cells harvested at day 20 of culture showing expressing of CD45 but lack of myeloid commitment, as evidenced by staining for CD13, CD14, and CD11c. Open histograms show levels of background staining using isotype-matched control antibodies. (b) Appearance of CD45int cells at day 27 of culture, accompanied by the upregulation of myeloid-specific markers. (c) Photomicrograph, taken at day 28 of culture, showing the morphology of DC, including veils of cytoplasm and long dendrites (inset) (×40 magnification). (d) Cells harvested at day 33 of culture, showing the appearance of a CD45hi population containing predominantly DC progenitors expressing CD14, CD11c, CD86 and MHC class I. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.

From: Kathryn M. Silk, Alison J. Leishman, Kevin P. Nishimoto, Anita Reddy, and Paul J. Fairchild, “Rapamycin Conditioning of Dendritic Cells Differentiated from Human ES Cells Promotes a Tolerogenic Phenotype,”
Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 172420, 11 pages, 2012.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 5
Published customer image:
Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477) used for the evaluation of HLA DR expression on dendritic cells by flow cytometry.
Image caption:
Upregulation of DC class II expression by paclitaxel is not mediated via TLR-4. DC were cultured for 2 h with LPS or paclitaxel in the presence or absence of anti-TLR-4 Abs prior to washing and reculture for a further 24 h. Anti-TLR4 antibody did reduce class II expression after exposure to LPS, but not to paclitaxel at high dose. Numbers in parenthesis represent MFI of class II detection. Data is representative of 3 independent experiments from 3 donors.

From: John J, Ismail M, Riley C, Askham J, Morgan R, Melcher A, Pandha H. Differential effects of Paclitaxel on dendritic cell function.
BMC Immunol. 2010 Mar 19;11:14.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 6
Published customer image:
Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477) used for the treatment of human mononuclear cells in vitro
.Image caption:
Inhibition of proliferation by anti-MHC class II antibody. Mononuclear cells from cord blood (■) or adult PBMC (□) were preincubated with MAb MCA477, and assays of proliferation to different antigens were carried out. Inhibition was expressed as the SI for treated cells compared to that for untreated cells, which was normalized to 100%. Each bar represents the mean and standard deviation from three assays.

From: Chia JS, You CM, Hu CY, Chiang BL, Chen JY.
Human T-cell responses to the glucosyltransferases of Streptococcus mutans. Clin Diagn Lab Immunol. 2001 Mar;8(2):441-5.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 7
Published customer image:
Mouse anti Human HLA DP/DQ/DR antibody, clone WR18 (MCA477) used for the evaluation of HLA class II histocompatibility antigen expression on cell populations by flow cytometry.
Image caption:
DC differentiation from H1 hESC.
Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.

From: Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ.
Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012;2012:172420.
doi: 10.1155/2012/172420.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 8
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and SBV515 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 9
Published customer image:
Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477) used for the treatment on monocytes in vitrodetection of MHC class II on
Image caption:
Ligand engagement of HLA class II molecules up regulates MyD88, IL-1R1, and TNF-α in CD14+ human monocytes treated with SEB, SEA, or mAb directed against MHC-class II molecules. (A) Transcriptional activation of MyD88, IL-1R1AcP and TNF-α in HLA-DR positive primary monocytes treated with 200 ng SEB/ml (optimum dose), mAbs (10 µg/ml, optimum dose) directed against MHC-class II molecule (anti-DR-DP-DQ or LB3.1) or unrelated control antibody OKT3 was examined by semi-quantitative RT-PCR. Data shown is one of 3 similar experiments; (B) Agonists binding to TLR4 or HLA class II molecules on CD14+ monocytes induced transcriptional up regulation of MyD88. Real time RT-PCR was used to determine relative expression of MyD88 normalized to the expression of β-actin. Expression levels are determined as means ± SD. Data presented as one of 3 similar experiments. Significance compared to untreated control (*) was assigned as P≤0.0001. (C) MHC class II molecule dependence of SEB- induced TNF- α gene expression. Pretreatment of monocytes in ice with anti-DR-DP-DQ at optimum dose (10 μg/ml) followed by SEB stimulation resulted in reduced TNF- α gene expression. Transcriptional activation of TNF- α expression normalized to the expression of β-actin. Expression levels of TNF- α are expressed as means ± SD. Significance was assigned (*) or (**) as P values ≤0.003 comparing untreated vs treatment groups with anti-DR-DP-DQ, SEB or SEB vs anti-DR-DP-DQ+SEB respectively. (D) Confocal images show expression of HLA-DR (green) and intracellular MyD88 (red) proteins in CD14+ monocytes treated with HLA class II- ligands or control antibody OKT3 for 16 h; Scale bar = 5 μm; (E) intracellular expression of MyD88 protein in activated monocytes. Primary monocytes (CD14+, CD3-) were activated as described earlier, permeabilized and labeled with primary MyD88 antibody followed by PE-labeled secondary antibody and analyzed by flow cytometry. Histogram represents a MHC class II ligand-induced increase in expression of MyD88 protein compared to non-MHC class II ligand (OKT3).

From: Kissner TL, Ruthel G, Alam S, Ulrich RG, Fernandez S, et al. (2011) Activation of MyD88 Signaling upon Staphylococcal Enterotoxin Binding to MHC Class II Molecules.
PLoS ONE 6(1): e15985.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 10
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and StarBright Violet 670 conjugated Rat anti Human HLA DP DQ DR (MCA477SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 11
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and SBV440 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBV440). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 12
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD19 (MCA1940SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD19 (MCA1940SBB700) and StarBright Violet 610 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 13
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD19 (MCA1940SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD19 (MCA1940SBB700) and StarBright Violet 570 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 14
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440) and StarBright Violet 710 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 15
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and StarBright Violet 790 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 16
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 475 conjugated Mouse anti Human HLA AP DQ DR (MCA477SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 17
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD19 (MCA1940SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD19 (MCA1940SBV710) and StarBright UltraViolet 400 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 18
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD19 (MCA1940SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD19 (MCA1940SBV710) and StarBright UltraViolet 400 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 19
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400) and StarBright Violet 760 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 20
StarBright Violet 515 conjugated Mouse anti Human CD19 (MCA1940SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD19 (MCA1940SBV515) and StarBright UltraViolet 665 conjugated Mouse anti Human HLA DP DR DQ (MCA477SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 21
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD19 (MCA1940SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD19 (MCA1940SBV610) and StarBright UltraViolet 445 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 22
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and StarBright UltraViolet 795 conjugated Mouse anti human HLA DP DQ DR (MCA477SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 23
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD19 (MCA1940SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD19 (MCA1940SBV710) and StarBright UltraViolet 510 conjugated Mouse anti Human HLA DP DR DQ (MCA477SBUV510). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 24
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD19 (MCA1940SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD19 (MCA1940SBV515) and StarBright UltraViolet 575 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 25
Figure A. StarBright Ultra Violet 400 conjugated Mouse anti Human CD14 (MCA1568SBUV400). Figure B. StarBright Ultra Violet 400 conjugated Mouse anti Human CD14 (MCA1568SBUV400) and StarBright UltraViolet 605 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBUV605). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 26
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD19 (MCA1940A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD19 (MCA1940A647) and StarBright Blue 580 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 27
Figure A. StarBright Ultra Violet 510 conjugated Mouse anti Human CD19 (MCA1940SBUV510). Figure B. StarBright Ultra Violet 510 conjugated Mouse anti Human CD19 (MCA1940SBUV510) and StarBright Blue 615 conjugated Mouse anti Human HLA DP DR DQ (MCA477SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 28
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD19 (MCA1940SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD19 (MCA1940SBV515) and StarBright Blue 675 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 29
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440) and StarBright Blue 765 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 30
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD19 (MCA1940A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD19 (MCA1940A647) and StarBright Blue 810 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBB810). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 31
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD19 (MCA1940SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD19 (MCA1940SBV790) and StarBright Yellow 575 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 32
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440) and StarBright Yellow 720 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBY720). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 33
Figure A. StarBright Blue 765 conjugated Mouse anti Human CD19 (MCA1940SBB765). Figure B. StarBright Blue 765 conjugated Mouse anti Human CD19 (MCA1940SBB765) and StarBright Yellow 605 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 34
Figure A. StarBright Blue 580 conjugated Mouse anti Human CD19 (MCA1940SBB580). Figure B. StarBright Blue 580 conjugated Mouse anti Human CD19 (MCA1940SBB580) and StarBright Yellow 665 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 35
Figure A. StarBright UltraViolet 510 conjugated Mouse anti Human CD19 (MCA1940SBV610). Figure B. StarBright UltraViolet 510 conjugated Mouse anti Human CD19 (MCA1940SBV610) and StarBright Yellow 800 conjugated Mouse anti Human HLADPDQDR (MCA477SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human HLA DP DQ DR Antibody, clone WR18 gallery image 36
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD14 (MCA1568SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD14 (MCA1568SBV515) and StarBright Red 775 conjugated Mouse anti Human HLA DPDQDR (MCA477SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human HLA DP DQ DR:StarBright UltraViolet 400

Product Type
Monoclonal Antibody
Clone
WR18
Isotype
IgG2a
Specificity
HLA DP DQ DR

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Product Code Applications Pack Size List Price Your Price Qty
MCA477SBUV400
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F 100 Tests/0.5ml loader
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Mouse anti Human HLA DP DQ DR antibody, clone WR18 reacts with a monomorphic determinant common to DP, DQ and DR beta chains, which are expressed by antigen presenting cells, B cells, monocytes and activated T lymphocytes.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.

Target Species
Human
Product Form
Purified IgG conjugated to StarBright UltraViolet 400 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Human HLA Class II (DP, DQ, DR).
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells from NS0 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright UltraViolet 400 335 394
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for HLA DP DQ DR antibody

  1. Moore, K. et al. (1987) Use of the monoclonal antibody WR17, identifying the CD37 gp40-45 Kd antigen complex, in the diagnosis of B-lymphoid malignancy.
    J Pathol 152:13-21.
  2. Trefzer, U. et al. (2000) Hybrid cell vaccination for cancer immune therapy: first clinical trial with metastatic melanoma.
    Int J Cancer. 85 (5): 618-26.
  3. Palmer, K.J. et al. (2000) Interferon-alpha (IFN-alpha) stimulates anti-melanoma cytotoxic T lymphocyte (CTL) generation in mixed lymphocyte tumour cultures (MLTC).
    Clin Exp Immunol. 119: 412-8.
  4. Chia, J.S. et al. (2001) Human T-cell responses to the glucosyltransferases of Streptococcus mutans.
    Clin Diagn Lab Immunol. 8: 441-5.
  5. Keating, S. et al. (2002) The lytic cycle of Epstein-Barr virus is associated with decreased expression of cell surface major histocompatibility complex class I and class II molecules.
    J Virol. 76: 8179-88.
  6. Elias, F. et al. (2003) Strong cytosine-guanosine-independent immunostimulation in humans and other primates by synthetic oligodeoxynucleotides with PyNTTTTGT motifs.
    J Immunol. 171: 3697-704.
  7. Llewelyn, M. et al. (2004) HLA class II polymorphisms determine responses to bacterial superantigens.
    J Immunol. 172 (3): 1719-26.
  8. Neumann F et al. (2004) Identification of an HLA-DR-restricted peptide epitope with a promiscuous binding pattern derived from the cancer testis antigen HOM-MEL-40/SSX2.
    Int J Cancer. 112 (4): 661-8.
    9. View The Latest Product References
  9. Neumann, F. et al. (2004) Identification of an antigenic peptide derived from the cancer-testis antigen NY-ESO-1 binding to a broad range of HLA-DR subtypes.
    Cancer Immunol Immunother. 53 (7): 589-99.
  10. Adamski, J. (2004) 17{beta}-Estradiol Inhibits Class II MHC Expression: Influence on Histone Modifications and CBP Recruitment to the Class II MHC Promoter
    Molecular Endocrinology 18:1963
  11. Iking-Konert C et al. (2005) Transdifferentiation of polymorphonuclear neutrophils to dendritic-like cells at the site of inflammation in rheumatoid arthritis: evidence for activation by T cells.
    Ann Rheum Dis. 64 (10): 1436-42.
  12. Hayman, M.W. et al. (2006) Soluble human leukocyte antigen: a diagnostic indicator of rheumatoid arthritis?
    J Immunol Methods. 315 (1-2): 19-26.
  13. Chang, Y.C. et al. (2008) Epigenetic control of MHC class II expression in tumor-associated macrophages by decoy receptor 3.
    Blood. 111: 5054-63.
  14. del Pilar Martin, M. et al. (2008) Decrease in the numbers of dendritic cells and CD4+ T cells in cerebral perivascular spaces due to natalizumab.
    Arch Neurol. 65 (12): 1596-603.
  15. Litzinger, M.T. et al. (2009) Chronic lymphocytic leukemia (CLL) cells genetically modified to express B7-1, ICAM-1, and LFA-3 confer APC capacity to T cells from CLL patients.
    Cancer Immunol Immunother. 58: 955-65.
  16. John, J. et al. (2010) Differential effects of Paclitaxel on dendritic cell function.
    BMC Immunol. 11:14.
  17. Sadallah, S. et al. (2011) Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells.
    J Immunol. 186: 6543-52.
  18. Kissner, T.L. et al. (2011) Activation of MyD88 Signaling upon Staphylococcal Enterotoxin Binding to MHC Class II Molecules.
    PLoS One. 6: e15985.
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Flow Cytometry

Functional Assays

Immunohistology - Paraffin

MCA477SBUV400

100006075

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