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CD11c antibody | BU15

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Mouse anti Human CD11c:StarBright Blue 700

Product Type
Monoclonal Antibody
Clone
BU15
Isotype
IgG1
Specificity
CD11c

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Product Code Applications Pack Size List Price Your Price Qty
MCA2087SBB700
Datasheet Datasheet
SDS Safety Datasheet SDS
F * 100 Tests/0.5ml loader
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Anti Human CD11c Antibody, clone BU15 thumbnail image 1 Anti Human CD11c Antibody, clone BU15 thumbnail image 2 Anti Human CD11c Antibody, clone BU15 thumbnail image 3 Anti Human CD11c Antibody, clone BU15 thumbnail image 4 Anti Human CD11c Antibody, clone BU15 thumbnail image 5 Anti Human CD11c Antibody, clone BU15 thumbnail image 6 Anti Human CD11c Antibody, clone BU15 thumbnail image 7 Anti Human CD11c Antibody, clone BU15 thumbnail image 8 Anti Human CD11c Antibody, clone BU15 thumbnail image 9
Anti Human CD11c Antibody, clone BU15 gallery image 1
Figure A. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody, clone WR18 (MCA477F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE).
Figure B. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody, clone WR18 (MCA477F) and RPE conjugated Mouse anti Human CD11c antibody, clone Bu15 (MCA2087PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).
Anti Human CD11c Antibody, clone BU15 gallery image 2
Published customer image:
Alexa Fluor® 647 conjugated Mouse anti Human CD11c antibody, clone BU15 (MCA2087A647) used for the identification of accessory cells derived from human PBMCs by flow cytometry.
Image caption:
Accessory Cells Are Activated by iRBCs PBMCs from ten malaria-naïve donors were cultured with live iRBCs or E. coli LPS for 24 h or left unstimulated. Surface expression of HLA-DR, CD40, and CD80 on accessory cells was analysed by flow cytometry.
Accessory cells were defined as HLA-DR+ CD11c+ cells (A, left), and CD14+ monocytes and CD14− mDCs were selected for further analysis (A, right).

From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006)
Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria.
PLoS Pathog 2(12): e118.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD11c Antibody, clone BU15 gallery image 3
Published customer image:
Mouse anti Human CD11c antibody, clone Bu15 (MCA2087) used for the evaluation of CD11c expression on Huvecs following co-culture with B. malayi by flow cytometry.
Image caption:
Presence of C3 enhances Mf adherence.
Level of B. malayi Mf adherence to HUVEC after co-culturing for 24 hours (A) in the presence of 10% intact human serum or 10% human serum depleted of C3. (B) Mf were incubated in intact sera and C3 deposition was detected using FITC conjugated anti-C3 Ab. (C) HUVEC were stimulated with or without IFN-γ (10 ng/ml) for 48 hours prior to a 24 hour incubation with Mf. HUVEC were then analysed by FACS for surface expression of CR1 or CD11c. Data show mean fluorescence intensity (MFI) of CR1 and CD11c. (D) HUVEC were pre-treated either with an isotype control antibody or with anti-CR1 for 45 minutes immediately prior to Mf co-culture for 24 hours. In another experiment, HUVEC were pre-treated for 45 minutes in the presence or absence of 250μg/ml mannan prior to Mf co-culture. Mf binding was counted after 24 hours of co-culture. Data are shown as the mean ± standard deviation of three independent experiments and Student's t-test was used to analyse statistical significance where ** p<0.01.

From: Zhou H, Luby-Phelps K, Mickey BE, Habib AA, Mason RP, Zhao D (2009)
Dynamic Near-Infrared Optical Imaging of 2-Deoxyglucose Uptake by Intracranial Glioma of Athymic Mice.
PLoS ONE 4(11): e8051.
doi: 10.1371/journal.pntd.0005592
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD11c Antibody, clone BU15 gallery image 4
Human tonsil cryosection stained with Mouse anti Human CD11c antibody, clone Bu15 (MCA2087).
Anti Human CD11c Antibody, clone BU15 gallery image 5
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477SBUV400).
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human HLA DP DQ DR (MCA477SBUV400) and StarBright Violet 790 conjugated Mouse anti Human CD11c antibody, clone Bu15 (MCA2087SBV790). All experiments performed on human PBMCs gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Anti Human CD11c Antibody, clone BU15 gallery image 6
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477SBUV400).
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477SBUV400) and StarBright Blue 700 conjugated Mouse anti Human CD11c antibody, clone Bu15 (MCA2087SBB700). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Anti Human CD11c Antibody, clone BU15 gallery image 7
Figure A. StarBright Red 775 conjugated Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477SBR775).
Figure B. StarBright Red 775 conjugated Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477SBR775) and StarBright Violet 570 conjugated Mouse anti Human CD11c antibody, clone Bu15 (MCA2087SBV570).
All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD11c Antibody, clone BU15 gallery image 8
Figure A. StarBright Red 670 conjugated Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477SBR670).
Figure B. StarBright Red 670 conjugated Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477SBR670) and StarBright Violet 440 conjugated Mouse anti Human CD11c antibody, clone Bu15 (MCA2087SBV440). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Anti Human CD11c Antibody, clone BU15 gallery image 9
Figure A. StarBright Blue 810 conjugated Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477SBB810).
Figure B. StarBright Blue 810 conjugated Mouse anti Human HLA DP DQ DR antibody, clone WR18 (MCA477SBB810) and StarBright Violet 610 conjugated Mouse anti Human CD11c antibody, clone Bu15 (MCA2087SBV610). All experiments performed on red blood lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.

Filter by Application:

F C

Mouse anti Human CD11c antibody, clone Bu15 recognizes the integrin alphax subunit (ITGAX), a ~150 kDa glycoprotein also known as CD11 antigen-like family member C or Leu M5, CD11c is expressed by macrophages, monocytes, NK cells and most dendritic cells (Kohrgruber et al. 1999). CD11c is also expressed at a lower level by granulocytes.

CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b (Sadhu et al. 2007) and Junctional adhesion molecule-like (Bazzoni et al. 2011). CD11c is involved in cell-cell interactions during the inflammatory process and is important for monocyte adhesion and chemotaxis. CD11c also acts as a signalling receptor for polysaccharide (Inqualls et al. 1995)

CD11c is expressed by hairy cell leukaemia cells (Goodman et al. 2003; Nicolaou et al. 2003).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Blue 700 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Approx. Protein Concentrations
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Blue 700 473 703
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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References for CD11c antibody

  1. Hogg, N. et al. (1986) The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.
    Eur J Immunol. 16 (3): 240-8.
  2. Levy, O. et al. (2003) Critical role of the complement system in group B streptococcus-induced tumor necrosis factor alpha release.
    Infect Immun. 71: 6344-53.
  3. Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
    PLoS Pathog. 2: e118.
  4. Brown, D.P. et al. (2009) The inhibitory receptor LILRB4 (ILT3) modulates antigen presenting cell phenotype and, along with LILRB2 (ILT4), is upregulated in response to Salmonella infection.
    BMC Immunol. 10: 56.
  5. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
    J Biomed Biotechnol. 2012:172420.
  6. Herman, S. et al. (2012) Regulatory T cells form stable and long-lasting cell cluster with myeloid dendritic cells (DC).
    Int Immunol. 24 (7): 417-26.
  7. Xie, Z. et al. (2016) Human umbilical cord-derived mesenchymal stem cells elicit macrophages into an anti-inflammatory phenotype to alleviate insulin resistance in type 2 diabetic rats.
    Stem Cells. 34 (3): 627-39.
  8. Schroeder JH et al. (2017) Brugia malayi microfilariae adhere to human vascular endothelial cells in a C3-dependent manner.
    PLoS Negl Trop Dis. 11 (5): e0005592.
    9. View The Latest Product References
  9. Simões, R.D. et al. (2019) Effects of Regulatory T Cell Depletion on NK Cell Responses against Listeria monocytogenes in Feline Immunodeficiency Virus Infected Cats.
    Viruses. 11 (11)Oct 24 [Epub ahead of print].
  10. de Vries, R.D. et al. (2014) Measles vaccination of nonhuman primates provides partial protection against infection with canine distemper virus.
    J Virol. 88 (8): 4423-33.

Further Reading

  1. Larson, R.S. & Springer, T.A. (1990) Structure and function of leukocyte integrins.
    Immunol Rev. 114: 181-217.
  2. Loike, J.D. et al. (1991) CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the A alpha chain of fibrinogen.
    Proc Natl Acad Sci U S A. 88 (3): 1044-8.
  3. Sanchez-Madrid, F. and Corbi, A.L. (1992) Leukocyte integrins: structure, function and regulation of their activity.
    Seminars Cell Biol. 3: 199-210.

Flow Cytometry

Functional Assays

Synonyms
CD11
Integrin Alpha X Chain
UniProt
P20702
Entrez Gene
ITGAX
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0009887 organ morphogenesis
GO:0050900 leukocyte migration

MCA2087SBB700

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