HLA ABC antibody | YTH862.2

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Rat anti Human HLA ABC

Product Type
Monoclonal Antibody
Clone
YTH862.2
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA485G C F FN* IF IP 0.2 mg
Rat anti Human HLA ABC antibody, clone YTH862.2 recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.

Rat anti Human HLA ABC antibody, clone YTH862.2 may be able to induce apoptosis in activated but not resting T cells (Genestier et al. 1997).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
PHA activated human peripheral blood lymphocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.

Storage Information

Storage
Store at +4oC for one month or at -20oC if preferred.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of HLA ABC antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Functional Assays 1
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2b Negative Control MCA6006GA 0.1 mg F

Application Based External Images

Functional Assays

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for HLA ABC antibody

  1. Genestier, L. et al. (1997) Fas-independent apoptosis of activated T cells induced by antibodies to the HLA class I alpha1 domain.
    Blood. 90 (9): 3629-39.
  2. Genestier, L. et al. (1997) Antibodies to HLA class I alpha1 domain trigger apoptosis of CD40-activated human B lymphocytes.
    Blood. 90 (2): 726-35.
  3. Genestier, L. et al. (1998) Caspase-dependent ceramide production in Fas- and HLA class I-mediated peripheral T cell apoptosis.
    J Biol Chem. 273 (9): 5060-6.
  4. Liu, S. et al. (2005) Cross-talk between Schwann cells and neuroblasts influences the biology of neuroblastoma xenografts.
    Am J Pathol. 166 (3): 891-900.
  5. Schmidt, J. et al. (2008) Interrelation of inflammation and APP in sIBM: IL-1 beta induces accumulation of beta-amyloid in skeletal muscle.
    Brain. 131 (Pt 5): 1228-40.
  6. Muth, I.E. et al. (2009) Proinflammatory cell stress in sporadic inclusion body myositis muscle: overexpression of alphaB-crystallin is associated with amyloid precursor protein and accumulation of beta-amyloid.
    J Neurol Neurosurg Psychiatry. 80: 1344-9.
  7. Schmidt, J. et al. (2004) Upregulated inducible co-stimulator (ICOS) and ICOS-ligand in inclusion body myositis muscle: significance for CD8+ T cell cytotoxicity.
    Brain. 127: 1182-90.
  8. Schmidt, K. et al. (2016) Molecular treatment effects of alemtuzumab in skeletal muscles of patients with IBM.
    BMC Neurol. 16 (1): 48.
  9. Muth, I.E. et al. (2015) HMGB1 and RAGE in skeletal muscle inflammation: Implications for protein accumulation in inclusion body myositis.
    Exp Neurol. 271: 189-97.
  10. Schmidt, C.M. et al. (1997) Cytotoxic T lymphocyte recognition of HLA-G in mice.
    Hum Immunol. 55 (2): 127-39.
  11. Tanaka, J. et al. (2004) Cytolytic activity and regulatory functions of inhibitory NK cell receptor-expressing T cells expanded from granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cells.
    Blood. 104 (3): 768-74.
  12. Limozin, L. et al. (2016) A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation.
    Sci Rep. 6: 35193.