Rat anti Human HLA ABC antibody, clone YTH862.2
recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
Rat anti Human HLA ABC antibody, clone YTH862.2 may be able to induce apoptosis in activated but not resting T cells (Genestier et al. 1997
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- PHA activated human peripheral blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
- Store at +4oC for one month or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of HLA ABC antibody
|Functional Assays 1
|Immunohistology - Frozen
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Immunohistology - Frozen
Product Specific References
References for HLA ABC antibody
Genestier, L. et al. (1997) Fas-independent apoptosis of activated T cells induced by antibodies to the HLA class I alpha1 domain.
Blood. 90 (9): 3629-39.
Genestier, L. et al. (1997) Antibodies to HLA class I alpha1 domain trigger apoptosis of CD40-activated human B lymphocytes.
Blood. 90 (2): 726-35.
Genestier, L. et al. (1998) Caspase-dependent ceramide production in Fas- and HLA class I-mediated peripheral T cell apoptosis.
J Biol Chem. 273 (9): 5060-6.
Liu, S. et al. (2005) Cross-talk between Schwann cells and neuroblasts influences the biology of neuroblastoma xenografts.
Am J Pathol. 166 (3): 891-900.
Schmidt, J. et al. (2008) Interrelation of inflammation and APP in sIBM: IL-1 beta induces accumulation of beta-amyloid in skeletal muscle.
Brain. 131 (Pt 5): 1228-40.
Muth, I.E. et al. (2009) Proinflammatory cell stress in sporadic inclusion body myositis muscle: overexpression of alphaB-crystallin is associated with amyloid precursor protein and accumulation of beta-amyloid.
J Neurol Neurosurg Psychiatry. 80: 1344-9.
Schmidt, J. et al. (2004) Upregulated inducible co-stimulator (ICOS) and ICOS-ligand in inclusion body myositis muscle: significance for CD8+ T cell cytotoxicity.
Brain. 127: 1182-90.
Schmidt, K. et al. (2016) Molecular treatment effects of alemtuzumab in skeletal muscles of patients with IBM.
BMC Neurol. 16 (1): 48.
Muth, I.E. et al. (2015) HMGB1 and RAGE in skeletal muscle inflammation: Implications for protein accumulation in inclusion body myositis.
Exp Neurol. 271: 189-97.
Schmidt, C.M. et al. (1997) Cytotoxic T lymphocyte recognition of HLA-G in mice.
Hum Immunol. 55 (2): 127-39.
Tanaka, J. et al. (2004) Cytolytic activity and regulatory functions of inhibitory NK cell receptor-expressing T cells expanded from granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cells.
Blood. 104 (3): 768-74.
Limozin, L. et al. (2016) A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation.
Sci Rep. 6: 35193.