HLA ABC antibody | YTH862.2
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
Rat anti Human HLA ABC antibody, clone YTH862.2 may be able to induce apoptosis in activated but not resting T cells (Genestier et al. 1997).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- PHA activated human peripheral blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
Storage Information
- Storage
- Store at +4oC for one month or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of HLA ABC antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/100 | |
Functional Assays 1 | |||
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunoprecipitation |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
![]() |
|
Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
![]() |
|
Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
![]() |
|
Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
![]() |
|
Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
![]() |
|
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
![]() |
|
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
![]() |
|
Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
![]() |
|
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
![]() |
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
![]() |
Product Specific References
References for HLA ABC antibody
-
Genestier, L. et al. (1997) Fas-independent apoptosis of activated T cells induced by antibodies to the HLA class I alpha1 domain.
Blood. 90 (9): 3629-39. -
Genestier, L. et al. (1997) Antibodies to HLA class I alpha1 domain trigger apoptosis of CD40-activated human B lymphocytes.
Blood. 90 (2): 726-35. -
Genestier, L. et al. (1998) Caspase-dependent ceramide production in Fas- and HLA class I-mediated peripheral T cell apoptosis.
J Biol Chem. 273 (9): 5060-6. -
Liu, S. et al. (2005) Cross-talk between Schwann cells and neuroblasts influences the biology of neuroblastoma xenografts.
Am J Pathol. 166 (3): 891-900. -
Schmidt, J. et al. (2008) Interrelation of inflammation and APP in sIBM: IL-1 beta induces accumulation of beta-amyloid in skeletal muscle.
Brain. 131 (Pt 5): 1228-40. -
Muth, I.E. et al. (2009) Proinflammatory cell stress in sporadic inclusion body myositis muscle: overexpression of alphaB-crystallin is associated with amyloid precursor protein and accumulation of beta-amyloid.
J Neurol Neurosurg Psychiatry. 80: 1344-9. -
Schmidt, J. et al. (2004) Upregulated inducible co-stimulator (ICOS) and ICOS-ligand in inclusion body myositis muscle: significance for CD8+ T cell cytotoxicity.
Brain. 127: 1182-90. -
Schmidt, K. et al. (2016) Molecular treatment effects of alemtuzumab in skeletal muscles of patients with IBM.
BMC Neurol. 16 (1): 48. -
Muth, I.E. et al. (2015) HMGB1 and RAGE in skeletal muscle inflammation: Implications for protein accumulation in inclusion body myositis.
Exp Neurol. 271: 189-97. -
Schmidt, C.M. et al. (1997) Cytotoxic T lymphocyte recognition of HLA-G in mice.
Hum Immunol. 55 (2): 127-39. -
Tanaka, J. et al. (2004) Cytolytic activity and regulatory functions of inhibitory NK cell receptor-expressing T cells expanded from granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cells.
Blood. 104 (3): 768-74. -
Limozin, L. et al. (2016) A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation.
Sci Rep. 6: 35193.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra