FOXA1 antibody | FOXA1/1512

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Anti FOXA1 Antibody, clone FOXA1/1512 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti FOXA1 Antibody, clone FOXA1/1512 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti FOXA1 Antibody, clone FOXA1/1512 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti FOXA1 Antibody, clone FOXA1/1512 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti FOXA1 Antibody, clone FOXA1/1512 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of FOXA1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti FOXA1 antibody (**VMA00663**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 255 second and (B) 30 second exposure.

Anti FOXA1 Antibody, clone FOXA1/1512 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of HepG2 whole cell lysate probed with FOXA1 antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 15 second exposure.

Anti FOXA1 Antibody, clone FOXA1/1512 (PrecisionAb Monoclonal Antibody) gallery image 3 — Western blot analysis of whole cell lysates probed with FOXA1 antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 192 second exposure.

Anti FOXA1 Antibody, clone FOXA1/1512 (PrecisionAb Monoclonal Antibody) gallery image 4 — Normalized signal intensity of FOXA1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Mouse anti FOXA1

Product Type: PrecisionAb Monoclonal
Clone: FOXA1/1512
Isotype: IgG1
Format: Purified
Specificity: FOXA1

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00663 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human FOXA1 antibody recognizes FOXA1, also known as Hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, TCF-3A or transcription factor 3A.

FOXA1 encodes a member of the forkhead class of DNA-binding proteins.

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver (provided by RefSeq, Jul 2008).

Mouse anti Human FOXA1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti FOXA1 antibody detects a band of approximately 50 kDa in HepG2 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant human FOXA1 (aa 467-614)
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P55317
Entrez Gene: FOXA1
GO Terms: GO:0003690 double-stranded DNA binding; GO:0003704 specific RNA polymerase II transcription factor activity; GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity; GO:0005667 transcription factor complex; GO:0006338 chromatin remodeling; GO:0009790 embryo development; GO:0007389 pattern specification process; GO:0008134 transcription factor binding; GO:0008301 DNA bending activity; View More GO Terms; GO:0008634 negative regulation of survival gene product expression; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010843 promoter binding; GO:0016563 transcription activator activity; GO:0016566 specific transcriptional repressor activity; GO:0019904 protein domain specific binding; GO:0032355 response to estradiol stimulus; GO:0033148 positive regulation of estrogen receptor signaling pathway; GO:0042593 glucose homeostasis; GO:0045666 positive regulation of neuron differentiation; GO:0045931 positive regulation of mitotic cell cycle; GO:0048665 neuron fate specification; GO:0051091 positive regulation of transcription factor activity; GO:0060441 epithelial tube branching involved in lung morphogenesis; GO:0060487 lung epithelial cell differentiation; GO:0060528 secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development; GO:0060738 epithelial-mesenchymal signaling involved in prostate gland development; GO:0060740 prostate gland epithelium morphogenesis; GO:0060741 prostate gland stromal morphogenesis; GO:0060743 epithelial cell maturation involved in prostate gland development