ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody | F04/4G10

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Mouse anti ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187)

ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody recognizes ERK1 phosphorylated at thr202 and tyr204, and ERK2 at thr185 and tyr187.

Product Type
PrecisionAb Monoclonal
Clone
F04/4G10
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00713
Datasheet
WB
SDS
100 µl loader

Mouse anti Human ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody recognizes ERK1, also known as MAPK3, when phosphorylated at threonine 202 and tyrosine 204, and ERK2, also known as MAPK1, when phosphorylated at threonine 185 and tyrosine 187.

MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of substrates containing a Pro-Xxx-Ser/Thr-Pro sequence (provided by RefSeq, Jul 2008). ERK1/2 are activated by phosphorylation by MAPK or ERK kinases (MEKs): ERK1 on threonine 202 and tyrosine 204 and ERK2 on threonine 185 and tyrosine 187 (Butch and Guan 1996).

Mouse anti Human ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody detects bands of 42 and 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) detects bands of approximately 42 & 44 kDa in EGF-treated HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the phosphorylated sites
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P27361
P28482
Entrez Gene
MAPK3
MAPK1
GO Terms
GO:0000186 activation of MAPKK activity
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004707 MAP kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006361 transcription initiation from RNA polymerase I promoter
GO:0007049 cell cycle
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0030168 platelet activation
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
GO:0003677 DNA binding
GO:0006350 transcription
GO:0006917 induction of apoptosis
GO:0007268 synaptic transmission
GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
GO:0070371 ERK1 and ERK2 cascade
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml