ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody | F04/4G10
Fig B. Normalized signal intensity of phosphorylated ERK2 (pThr185/pTyr187) probed with Mouse anti ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody (VMA00713) in the presence or absence of λ phosphatase.
Mouse anti ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187)
- Product Type
- PrecisionAb Monoclonal
- Clone
- F04/4G10
- Isotype
- IgG1
- Format
- Purified
- Specificity
- ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187)
Antibody Quality and Performance Guarantee
| Mouse anti Human ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody recognizes ERK1, also known as MAPK3, when phosphorylated at threonine 202 and tyrosine 204, and ERK2, also known as MAPK1, when phosphorylated at threonine 185 and tyrosine 187. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of substrates containing a Pro-Xxx-Ser/Thr-Pro sequence (provided by RefSeq, Jul 2008). ERK1/2 are activated by phosphorylation by MAPK or ERK kinases (MEKs): ERK1 on threonine 202 and tyrosine 204 and ERK2 on threonine 185 and tyrosine 187 (Butch and Guan 1996). Mouse anti Human ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) antibody detects bands of 42 and 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Mouse anti ERK1 (pThr202/pTyr204)/ERK2 (pThr185/pTyr187) detects bands of approximately 42 & 44 kDa in EGF-treated HEK293 cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to the phosphorylated sites
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P27361
- P28482
- Entrez Gene
- MAPK3
- MAPK1
- GO Terms
- GO:0000186 activation of MAPKK activity
- GO:0000187 activation of MAPK activity
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004707 MAP kinase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006361 transcription initiation from RNA polymerase I promoter
- View More GO Terms
- GO:0007049 cell cycle
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007265 Ras protein signal transduction
- GO:0007411 axon guidance
- GO:0008063 Toll signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0030168 platelet activation
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
- GO:0003677 DNA binding
- GO:0006350 transcription
- GO:0006917 induction of apoptosis
- GO:0007268 synaptic transmission
- GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
- GO:0070371 ERK1 and ERK2 cascade
VMA00713
100004599
64591946
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