ErbB2 antibody | 4D5-8
0.5 μg of Human Anti-ErbB2 Antibody, MCA6092 (trastuzumab biosimilar, research grade) and trastuzumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-ErbB2 Antibody, MCA6092 (trastuzumab biosimilar, research grade) and trastuzumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl; flow rate: 200 μl/min; inj. volume [μl]: 2; temp.: 25 °C; detection: 280 nm; sample: mAb (0.5-5 mg/ml)
A microtiter plate was coated overnight with Human Anti-Trastuzumab Antibody (clone AbD18018, HCA168) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of trastuzumab (therapeutic reference product) or Human Anti-ErbB2 Antibody, MCA6092 (trastuzumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Trastuzumab Antibody (clone AbD18018_hIgG1, HCA177) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®.
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PP E ResetHuman anti ErbB2 (Trastuzumab Biosimilar)
- Product Type
- Monoclonal Antibody
- Clone
- 4D5-8
- Isotype
- IgG1
- Specificity
- ErbB2
Antibody Quality and Performance Guarantee
| Human Anti-ErbB2 antibody, clone 4D5-8 is a research grade biosimilar of the monoclonal antibody drug trastuzumab. This product is supplied in a research grade format, i.e. in PBS buffer with preservative, it is not manufactured or formulated in the same way as the therapeutic reference product. A preservative free version is available on request. This product is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic trastuzumab antibody clone 4D5-8. Human Anti-ErbB2 antibody, clone 4D5-8 is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-trastuzumab antibodies, for example HCA168 and HCA167. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-trastuzumab antibody in IgG1 format as a reference standard, for example HCA176 or HCA177. Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells. This product is NOT FOR THERAPEUTIC USE. View a summary of supporting anti-trastuzumab antibody products. |
- Target Species
- Human
- Product Form
- Human IgG1 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Source
- HEK
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Proclin 300
- Immunogen
- A431 cells (human epidermoid carcinoma) (over)expressing EGFR
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Further Reading
-
Pivot, X. et al. (2015) Challenges in the implementation of trastuzumab biosimilars: an expert panel's recommendations.
Anticancer Drugs. 26 (10): 1009-16.
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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