ErbB2 antibody | 4D5-8

Human anti ErbB2 (Trastuzumab Biosimilar)

Product Type
Monoclonal Antibody
Clone
4D5-8
Isotype
IgG1
Specificity
ErbB2

Product Code Applications Pack Size List Price Your Price Qty
MCA6092
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Human Anti-ErbB2 antibody, clone 4D5-8 is a research grade biosimilar of the monoclonal antibody drug trastuzumab. This product is supplied in a research grade format, i.e. in PBS buffer with preservative, it is not manufactured or formulated in the same way as the therapeutic reference product. A preservative free version is available on request.

This product is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic trastuzumab antibody clone 4D5-8.

Human Anti-ErbB2 antibody, clone 4D5-8 is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-trastuzumab antibodies, for example HCA168 and HCA167. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-trastuzumab antibody in IgG1 format as a reference standard, for example HCA176 or HCA177.

Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

This product is NOT FOR THERAPEUTIC USE.

View a summary of supporting anti-trastuzumab antibody products.

Target Species
Human
Product Form
Human IgG1 (kappa) antibody - liquid
Preparation
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Proclin 300
Immunogen
A431 cells (human epidermoid carcinoma) (over)expressing EGFR
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Trastuzumab HCA166 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Trastuzumab
Human anti Trastuzumab HCA167 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Trastuzumab
Human anti Trastuzumab HCA168 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Trastuzumab
Human anti Trastuzumab HCA169 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Trastuzumab
Human anti Trastuzumab HCA176 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Trastuzumab
Human anti Trastuzumab:HRP HCA176P E 0.1 mg loader
List Price Your Price
loader
Description Human anti Trastuzumab:HRP
Human anti Trastuzumab HCA177 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Trastuzumab
Human anti Trastuzumab:HRP HCA177P E 0.1 mg loader
List Price Your Price
loader
Description Human anti Trastuzumab:HRP
Human anti Trastuzumab (Drug/Target Complex) HCA263 E 0.1 mg loader
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Description Human anti Trastuzumab (Drug/Target Complex)
Human anti Trastuzumab HCA270 E 0.1 mg loader
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Description Human anti Trastuzumab
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit

Further Reading

  1. Pivot, X. et al. (2015) Challenges in the implementation of trastuzumab biosimilars: an expert panel's recommendations.
    Anticancer Drugs. 26 (10): 1009-16.

Licensed Use
For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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