Trastuzumab antibody | AbD16712_hIgG1
A microtiter plate was coated overnight with trastuzumab at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added, spiked with increasing concentrations of Human Anti-Trastuzumab Antibody, clone AbD16712_hIgG1 (HCA176). Detection was performed using HRP conjugated trastuzumab in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase substrate. HRP conjugation of the trastuzumab used in this assay was performed using a LYNX Rapid HRP Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with either Human Anti-Trastuzumab, clone AbD16712 (HCA166) (Black) or Human Anti-Trastuzumab, clone AbD18141 (HCA169) (Red) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed using HRP conjugated Human Anti-Trastuzumab Antibody, clone AbD16712_hIgG1 (HCA176P) at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A), and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% milk powder, detection was performed using HRP conjugated Human Anti-Trastuzumab Antibody, clone AbD16712_hIgG1 (HCA176P) at a concentration of 1 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
Human anti Trastuzumab:HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD16712_hIgG1
- Isotype
- IgG1
- Specificity
- Trastuzumab
Antibody Quality and Performance Guarantee
| Human anti Trastuzumab, clone AbD16712_hIgG1, is an anti-idiotypic antibody that specifically recognizes the humanized monoclonal antibody drug trastuzumab, also known as Herceptin®. Clone AbD16712_hIgG can be used to measure the levels of trastuzumab and biosimilar products using bioanalytical assays. In the HRP-conjugated format clone AbD16712_hIgG1 is ideal for use as a detection antibody in pharmacokinetic (PK) assays. It is a fully human antibody and therefore may also be used as a calibrator in immune response assays to measure the anti-drug-antibody (ADA) response in patient sera. Trastuzumab is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells View a summary of all anti-trastuzumab antibodies. |
- Product Form
- Human IgG1 antibody selected from the HuCAL phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Trastuzumab (Herceptin®)
- Affinity
- The intrinsic affinity of the monovalent form of this antibody in an unconjugated format is 0.4 nM as measured by real time, label-free molecular interaction analysis on immobilized trastuzumab.
- Approx. Protein Concentrations
- Antibody concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used as a detection reagent in a sandwich ELISA together with HCA169 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Trastuzumab | HCA166 | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Human anti Trastuzumab | ||||||
| Human anti Trastuzumab | HCA169 | E | 0.1 mg |
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| Description | Human anti Trastuzumab | ||||||
References for Trastuzumab antibody
-
Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118 -
Iwamoto, N. et al. (2018) Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.
Anal Biochem. 540-541: 30-7.
- Synonyms
- HERCEPTIN®
- Licensed Use
- For in vitro. research purposes and for commercial applications for the provision of in vitro. testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
HCA176P
151996
158343
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