EIF5A antibody | AB01/2G8
Mouse anti EIF5A
- Product Type
- PrecisionAb Monoclonal
- Clone
- AB01/2G8
- Isotype
- IgG2b
- Format
- Purified
- Specificity
- EIF5A
Mouse anti EIF5A antibody recognizes eukaryotic translation initiation factor 5A-1. EIF5A is an abundant, essential translation factor which functions in elongation and termination during protein synthesis (Schuller et al. 2018). EIF5A is involved in the cell cycle, apoptosis, and in viral replication, notably replication of HIV-1 (Mathews and Hershey 2015), and in autophagosome formation, mediating autophagy (Lubas et al. 2018). It is the only eukaryotic protein known to follow a rare but essential post-translational modification called hypusination (Pelechano and Alepuz 2017). EIF5A is a critical regulator of cell growth and development of tumors (Puleston et al. 2019). For example, EIF5A promotes metastasis in pancreatic cancer and mediates chemoresistance (Wang et al. 2019). |
- Target Species
- Human
- Western Blotting
- Mouse anti EIF5A antibody detects a band of approximately 17 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-154 of human EIF5A
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
Immunofluorescence | |||
Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Goat anti Mouse IgG (H/L):HRP |
- UniProt
- P63241
- Entrez Gene
- EIF5A
- GO Terms
- GO:0003746 translation elongation factor activity
- GO:0008612 peptidyl-lysine modification to hypusine
- GO:0005829 cytosol
- GO:0005642 annulate lamellae
- GO:0005643 nuclear pore
- GO:0005789 endoplasmic reticulum membrane
- GO:0006406 mRNA export from nucleus
- GO:0006452 translational frameshifting
- GO:0006611 protein export from nucleus
- View More GO Terms
- GO:0006917 induction of apoptosis
- GO:0008284 positive regulation of cell proliferation
- GO:0017070 U6 snRNA binding
- GO:0043022 ribosome binding
- GO:0043687 post-translational protein modification
- GO:0045901 positive regulation of translational elongation
- GO:0045905 positive regulation of translational termination
- GO:0047485 protein N-terminus binding
- GO:0055085 transmembrane transport
VMA00857
100004559If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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