EIF5A antibody | AB01/2G8

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Mouse anti EIF5A

Product Type
PrecisionAb Monoclonal
Clone
AB01/2G8
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00857
Datasheet
WB
SDS
100 µl loader

Mouse anti EIF5A antibody recognizes eukaryotic translation initiation factor 5A-1.

EIF5A is an abundant, essential translation factor which functions in elongation and termination during protein synthesis (Schuller et al. 2018). EIF5A is involved in the cell cycle, apoptosis, and in viral replication, notably replication of HIV-1 (Mathews and Hershey 2015), and in autophagosome formation, mediating autophagy (Lubas et al. 2018). It is the only eukaryotic protein known to follow a rare but essential post-translational modification called hypusination (Pelechano and Alepuz 2017). EIF5A is a critical regulator of cell growth and development of tumors (Puleston et al. 2019). For example, EIF5A promotes metastasis in pancreatic cancer and mediates chemoresistance (Wang et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti EIF5A antibody detects a band of approximately 17 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-154 of human EIF5A
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P63241
Entrez Gene
EIF5A
GO Terms
GO:0003746 translation elongation factor activity
GO:0008612 peptidyl-lysine modification to hypusine
GO:0005829 cytosol
GO:0005642 annulate lamellae
GO:0005643 nuclear pore
GO:0005789 endoplasmic reticulum membrane
GO:0006406 mRNA export from nucleus
GO:0006452 translational frameshifting
GO:0006611 protein export from nucleus
GO:0006917 induction of apoptosis
GO:0008284 positive regulation of cell proliferation
GO:0017070 U6 snRNA binding
GO:0043022 ribosome binding
GO:0043687 post-translational protein modification
GO:0045901 positive regulation of translational elongation
GO:0045905 positive regulation of translational termination
GO:0047485 protein N-terminus binding
GO:0055085 transmembrane transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EIF5A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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