EIF5A antibody | AB01/2G8
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WB ResetMouse anti EIF5A
- Product Type
- PrecisionAb Monoclonal
- Clone
- AB01/2G8
- Isotype
- IgG2b
- Format
- Purified
- Specificity
- EIF5A
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| Mouse anti EIF5A antibody recognizes eukaryotic translation initiation factor 5A-1. EIF5A is an abundant, essential translation factor which functions in elongation and termination during protein synthesis (Schuller et al. 2018). EIF5A is involved in the cell cycle, apoptosis, and in viral replication, notably replication of HIV-1 (Mathews and Hershey 2015), and in autophagosome formation, mediating autophagy (Lubas et al. 2018). It is the only eukaryotic protein known to follow a rare but essential post-translational modification called hypusination (Pelechano and Alepuz 2017). EIF5A is a critical regulator of cell growth and development of tumors (Puleston et al. 2019). For example, EIF5A promotes metastasis in pancreatic cancer and mediates chemoresistance (Wang et al. 2019). |
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- Target Species
- Human
- Western Blotting
- Mouse anti EIF5A antibody detects a band of approximately 17 kDa in HEK293 cell lysates
- Species Cross-Reactivity
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Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-154 of human EIF5A
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P63241
- Entrez Gene
- EIF5A
- GO Terms
- GO:0003746 translation elongation factor activity
- GO:0008612 peptidyl-lysine modification to hypusine
- GO:0005829 cytosol
- GO:0005642 annulate lamellae
- GO:0005643 nuclear pore
- GO:0005789 endoplasmic reticulum membrane
- GO:0006406 mRNA export from nucleus
- GO:0006452 translational frameshifting
- GO:0006611 protein export from nucleus
- View More GO Terms
- GO:0006917 induction of apoptosis
- GO:0008284 positive regulation of cell proliferation
- GO:0017070 U6 snRNA binding
- GO:0043022 ribosome binding
- GO:0043687 post-translational protein modification
- GO:0045901 positive regulation of translational elongation
- GO:0045905 positive regulation of translational termination
- GO:0047485 protein N-terminus binding
- GO:0055085 transmembrane transport
View more products with EIF5A specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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