EIF5A antibody | AB01/2G8

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Anti EIF5A Antibody, clone AB01/2G8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti EIF5A Antibody, clone AB01/2G8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with EIF5A antibody (1/1000, **VMA00857**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to EIF5A (molecular weight 17 kDa)

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Mouse anti EIF5A

Product Type: PrecisionAb Monoclonal
Clone: AB01/2G8
Isotype: IgG2b
Format: Purified
Specificity: EIF5A

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00857 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti EIF5A antibody recognizes eukaryotic translation initiation factor 5A-1.

EIF5A is an abundant, essential translation factor which functions in elongation and termination during protein synthesis (Schuller et al. 2018). EIF5A is involved in the cell cycle, apoptosis, and in viral replication, notably replication of HIV-1 (Mathews and Hershey 2015), and in autophagosome formation, mediating autophagy (Lubas et al. 2018). It is the only eukaryotic protein known to follow a rare but essential post-translational modification called hypusination (Pelechano and Alepuz 2017). EIF5A is a critical regulator of cell growth and development of tumors (Puleston et al. 2019). For example, EIF5A promotes metastasis in pancreatic cancer and mediates chemoresistance (Wang et al. 2019).

Target Species: Human
Western Blotting: Mouse anti EIF5A antibody detects a band of approximately 17 kDa in HEK293 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-154 of human EIF5A
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P63241
Entrez Gene: EIF5A
GO Terms: GO:0003746 translation elongation factor activity; GO:0008612 peptidyl-lysine modification to hypusine; GO:0005829 cytosol; GO:0005642 annulate lamellae; GO:0005643 nuclear pore; GO:0005789 endoplasmic reticulum membrane; GO:0006406 mRNA export from nucleus; GO:0006452 translational frameshifting; GO:0006611 protein export from nucleus; View More GO Terms; GO:0006917 induction of apoptosis; GO:0008284 positive regulation of cell proliferation; GO:0017070 U6 snRNA binding; GO:0043022 ribosome binding; GO:0043687 post-translational protein modification; GO:0045901 positive regulation of translational elongation; GO:0045905 positive regulation of translational termination; GO:0047485 protein N-terminus binding; GO:0055085 transmembrane transport