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eEF1A1 antibody | F02/1E3

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Anti eEF1A1 Antibody, clone F02/1E3 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti eEF1A1 Antibody, clone F02/1E3 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti eEF1A1 Antibody, clone F02/1E3 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti eEF1A1 Antibody, clone F02/1E3 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with eEF1A1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to eEF1A1 (molecular weight 50 kDa).
Anti eEF1A1 Antibody, clone F02/1E3 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against eEF1A1 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti eEF1A1 antibody (VMA00511) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 150 second (chemiluminescent channel) and 60 second (rhodamine channel) exposure.
Anti eEF1A1 Antibody, clone F02/1E3 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of eEF1A1 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against eEF1A1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti eEF1A1

Product Type
PrecisionAb Monoclonal
Clone
F02/1E3
Isotype
IgG1
Format
Purified
Specificity
eEF1A1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00511
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human eEF1A1 antibody, clone F02/1E3 recognizes the elongation factor 1-alpha 1, also known as CTCL tumor antigen, cervical cancer suppressor 3, elongation factor 1 alpha subunit, elongation factor Tu, eukaryotic elongation factor 1 A-1, eukaryotic translation elongation factor 1 alpha 1-like 14, glucocorticoid receptor AF-1 specific elongation factor or leukocyte receptor cluster member 7.

The EEF1A1 gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome (Ditzel et al. 2000). EEF1A1 has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. (provided by RefSeq, Jul 2008).

Mouse anti Human eEF1A1 antibody, clone F02/1E3 detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti eEF1A1 detects a band of approximately 50 kDa in HEK293 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli derived recombinant protein corresponding to amino acids 1-462 of human eEF1A1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P68104
Entrez Gene
EEF1A1
GO Terms
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0003746 translation elongation factor activity
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0005853 eukaryotic translation elongation factor 1 complex

VMA00511

160801

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