Diacylglycerol Kinase Alpha antibody | OTI3G7

Mouse anti Diacylglycerol Kinase Alpha

Product Type
PrecisionAb Monoclonal
Clone
OTI3G7
Isotype
IgG2a
Format
Purified
Specificity
Diacylglycerol Kinase Alpha

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VMA00496
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Mouse anti Human diacylglycerol kinase alpha antibody recognizes diacylglycerol kinase alpha (DGKA), also known as 80 kDa diacylglycerol kinase or DAG kinase alpha.

The protein encoded by DGKA belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. (provided by RefSeq, Jul 2008).

Mouse anti Human diacylglycerol kinase alpha antibody detects a band of 83 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti diacylglycerol kinase alpha detects a band of approximately 83 kDa in Jurkat cell lysate.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin.
Immunogen
Full length recombinant human diacylglycerol kinase alpha (DGKA, NP_001336) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°>C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P23743
Entrez Gene
DGKA
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0004143 diacylglycerol kinase activity
GO:0035556 intracellular signal transduction
GO:0005524 ATP binding
GO:0007205 activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
GO:0030168 platelet activation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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