DDX3X antibody | AB04/4C5

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Mouse anti DDX3X

Product Type
PrecisionAb Monoclonal
Clone
AB04/4C5
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00543
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human DDX3X antibody recognizes the ATP-dependent RNA helicase DDX3X, also known as DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, DEAD/H box-3 or helicase-like protein 2.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3X gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. DDX3X has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between DDX3X and the Y-linked homolog. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2010).

Mouse anti Human DDX3X antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti DDX3X detects a band of approximately 75 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human DDX3X (amino acids 1 - 200)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
O00571 Related reagents
Entrez Gene
DDX3X Related reagents
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004004 ATP-dependent RNA helicase activity
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0016607 nuclear speck
GO:0044419 interspecies interaction between organisms
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DDX3X antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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