DDX3X antibody | AB04/4C5

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Anti DDX3X Antibody, clone AB04/4C5 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti DDX3X Antibody, clone AB04/4C5 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti DDX3X Antibody, clone AB04/4C5 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with DDX3X antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to DDX3X (molecular weight 75 kDa)

Anti DDX3X Antibody, clone AB04/4C5 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of DDX3X from HeLa whole cell lysate. The Input lane is 10% input; the DDX3X-IP lane is the immunoprecipitation with the DDX3X antibody (2.5 µg, **VMA00543**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA691). Western blot analysis was performed using DDX3X antibody (1/1,000, **VMA00543**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to DDX3X.

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WB IP

Mouse anti DDX3X

Product Type: PrecisionAb Monoclonal
Clone: AB04/4C5
Isotype: IgG2b
Format: Purified
Specificity: DDX3X

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00543 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human DDX3X antibody recognizes the ATP-dependent RNA helicase DDX3X, also known as DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, DEAD/H box-3 or helicase-like protein 2.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3X gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. DDX3X has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between DDX3X and the Y-linked homolog. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jul 2010).

Mouse anti Human DDX3X antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti DDX3X detects a band of approximately 75 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human DDX3X (amino acids 1 - 200)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: O00571
Entrez Gene: DDX3X
GO Terms: GO:0005515 protein binding; GO:0003677 DNA binding; GO:0003723 RNA binding; GO:0004004 ATP-dependent RNA helicase activity; GO:0005524 ATP binding; GO:0005737 cytoplasm; GO:0016607 nuclear speck; GO:0044419 interspecies interaction between organisms