Cytokeratin 18/19 antibody | AbD03748

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Human anti Cytokeratin 18/19

Product Type: PrecisionAb Monoclonal
Clone: AbD03748
Isotype: HuCAL Fab bivalent
Format: Purified
Specificity: Cytokeratin 18/19

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00011 Datasheet SDS SDS	E P * WB	100 µl		Log in
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Anti Cytokeratin 18/19 Antibody, clone AbD03748 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Cytokeratin 18/19 Antibody, clone AbD03748 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Cytokeratin 18/19 Antibody, clone AbD03748 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with cytokeratin 18/19 antibody (**VMA00011**) followed by detection with HRP conjugated Goat anti Human IgG F(ab')2 (1/2500, STAR126P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to cytokeratin 18/19 (molecular weight 48, 44 kDa).

Anti Cytokeratin 18/19 Antibody, clone AbD03748 (PrecisionAb Monoclonal Antibody) gallery image 2 — MCF-7 human breast adenocarcinoma cell lysate probed with Human anti Human cytokeratin 18/19 (**VMA00011**).

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Human anti Human cytokeratin 18/19 antibody recognizes human cytokeratin 18, a amino acid type I intermediate filament protein expressed by epithelial cells. The intermediate filaments are expressed as heterodimers in epithelial tissues. Cytokeratin 18 (CK18) is often co-expressed with cytokeratin 8 (CK8). The CK18/CK8 heterodimer is the major cytokeratin pair found in simple epithelia such as that found in the liver, intestine and breast ducts. CK18 plays an important role in maintaining epithelial cell integrity.

This clone has also been demonstrated to cross-react with cytokeratin 19. Human anti Human cytokeratin 18/19 antibody recognizes cytokeratin 18 as a band of ~48 kDa and cytokeratin 19 as a band of ~44 kDa in western blotting under reducing conditions.

Target Species: Human
Western Blotting: Anti cytokeratin 18/19 antibody detects bands of approximately 44 kDa in HeLa cell lysates.
Product Form: A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation: Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Source: E.coli
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant human cytokeratin 18
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunohistology - Paraffin ¹
Western Blotting			1/1000

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Learn about how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt: P05783; P08727
Entrez Gene: KRT18; KRT19
GO Terms: GO:0005515 protein binding; GO:0005198 structural molecule activity; GO:0007049 cell cycle; GO:0009653 anatomical structure morphogenesis; GO:0034451 centriolar satellite; GO:0043000 Golgi to plasma membrane CFTR protein transport; GO:0043066 negative regulation of apoptosis; GO:0044419 interspecies interaction between organisms; GO:0045095 keratin filament; View More GO Terms; GO:0048471 perinuclear region of cytoplasm