Cytokeratin 18/19 antibody | AbD03748
This clone has also been demonstrated to cross-react with cytokeratin 19. Human anti Human cytokeratin 18/19 antibody recognizes cytokeratin 18 as a band of ~48 kDa and cytokeratin 19 as a band of ~44 kDa in western blotting under reducing conditions.
- Target Species
- Western Blotting
- Anti cytokeratin 18/19 antibody detects bands of approximately 44 kDa in HeLa cell lysates.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
- Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Recombinant human cytokeratin 18
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0005198 structural molecule activity
- GO:0007049 cell cycle
- GO:0009653 anatomical structure morphogenesis
- GO:0034451 centriolar satellite
- GO:0043000 Golgi to plasma membrane CFTR protein transport
- GO:0043066 negative regulation of apoptosis
- GO:0044419 interspecies interaction between organisms
- GO:0045095 keratin filament
- GO:0048471 perinuclear region of cytoplasm
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences.
- For research purposes only
Applications of Cytokeratin 18/19 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Mouse anti Penta Histidine Tag:HRP||MCA5995P||E WB||125 µl|
|Goat anti Human IgG F(ab')2:HRP||STAR126P||E WB||0.5 ml|
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