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CD81 antibody | 1D6

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Anti Human CD81 Antibody, clone 1D6 thumbnail image 1 Anti Human CD81 Antibody, clone 1D6 thumbnail image 2 Anti Human CD81 Antibody, clone 1D6 thumbnail image 3 Anti Human CD81 Antibody, clone 1D6 thumbnail image 4 Anti Human CD81 Antibody, clone 1D6 thumbnail image 5 Anti Human CD81 Antibody, clone 1D6 thumbnail image 6 Anti Human CD81 Antibody, clone 1D6 thumbnail image 7
Anti Human CD81 Antibody, clone 1D6 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD19 (MCA1940F) and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928P647). Figure B. FITC conjugated Mouse anti Human CD19 (MCA1940F) and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD81 (MCA1847P647). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD81 Antibody, clone 1D6 gallery image 2
Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Alexa Fluor 488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Alexa Fluor 488 conjugated Mouse anti Human CD81 (MCA1847A488). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD81 Antibody, clone 1D6 gallery image 3
Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Alexa Fluor 647 conjugated Mouse anti Human CD81 (MCA1847A647). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD81 Antibody, clone 1D6 gallery image 4
Figure A. FITC conjugated Mouse anti Human CD19 (MCA1940F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human CD19 (MCA1940F) and RPE conjugated Mouse anti Human CD81 (MCA1847PE). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD81 Antibody, clone 1D6 gallery image 5
Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Mouse anti Human CD81 (MCA1847F). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 Cell Analyzer.
Anti Human CD81 Antibody, clone 1D6 gallery image 6
Published customer image:
Mouse anti Human CD81 antibody, clone 1D6 (MCA1847) used to evaluate CD81 expression in £T£-L1 cell lysates by western blotting.
Image caption:
Characterization of EVs released by 3T3‐L1 adipocytes. A) Cryo‐electron microscopy from Control (untreated), hypoxic or cells exposed to macrophage media (MCM). Scale bar: 100 nm. B) NTA of EVs from normoxic/ hypoxic cells and control/MCM‐exposed 3T3L1 adipocytes. C) Immunoblot of cellular lysates (L) or EVs from control, hypoxic, or MCM exposed cells. Equal amounts of lysate or EVs (1 μg) were separated by SDS‐PAGE and immunoblotted with specific antibodies as indicated.

From: Mleczko J, Ortega FJ, Falcon-Perez JM, Wabitsch M, Fernandez-Real JM, Mora S.
Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.
Mol Nutr Food Res. 2018 Mar;62(5). Epub 2018 Feb 20.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD81 Antibody, clone 1D6 gallery image 7
Published customer image:
Mouse anti Human CD81 antibody, clone 1D6 (MCA1847) used to evaluate CD81 expression in human extracellular vesicles by western blotting.
Image caption:
A) EVs from plasma (EV blood), EVs from human adipocytes (EV adipo), and adipocyte cellular lysates were separated by SDS‐PAGE and immunoblotted with antibodies for EV markers as indicated. B) Size distribution of plasma EVs by NTA. C) Insulin‐stimulated 2‐deoxyglucose uptake in SGBS cells treated with 2 × 105 EV mL–1 of EVs obtained from lean (n = 8) or obese subjects (n = 9). Data shows mean ± SD EV, each EV was tested in triplicate in two independent experiments, p = 0.0153 (t‐test).

From: Mleczko J, Ortega FJ, Falcon-Perez JM, Wabitsch M, Fernandez-Real JM, Mora S.
Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.
Mol Nutr Food Res. 2018 Mar;62(5). Epub 2018 Feb 20.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD81:RPE

Product Type
Monoclonal Antibody
Clone
1D6
Isotype
IgG1
Specificity
CD81

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Product Code Applications Pack Size List Price Your Price Qty
MCA1847PE
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F 100 Tests loader
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Mouse anti Human CD81 antibody, clone 1D6 recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular leucocytes including activation, proliferation and differentiation.

Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
Sheep
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
OCI-LY8 cells aggregated by 5A6 (another CD81 antibody)
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD81 antibody

  1. Schick, M.R. & Levy, S. (1993) The TAPA-1 molecule is associated on the surface of B cells with HLA-DR molecules.
    J Immunol. 151 (8): 4090-7.
  2. Levy, S. et al. (1998) CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system.
    Annu Rev Immunol. 16: 89-109.
  3. Flint, M. et al. (1999) Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.
    J Virol. 73:6235-44.
  4. Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
    Vet Immunol Immunopathol. 119: 123-30.
  5. Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
    Vet Immunol Immunopathol. 119: 115-22.
  6. Rohlena, J. et al. (2009) Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion.
    Cardiovasc Res. 81: 187-96.
  7. Parthasarathy, V. et al. (2009) Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.
    Immunology. 127: 237-48.
  8. Welton, J.L. et al (2010) Proteomics analysis of bladder cancer exosomes.
    Mol Cell Proteomics. 9: 1324-38.
    9. View The Latest Product References
  9. Ventress, J.K. et al. (2016) Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes.
    PLoS One. 11 (7): e0160387.
  10. Mleczko, J. et al. (2018) Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.
    Mol Nutr Food Res. 62 (5)Feb 20 [Epub ahead of print].

Flow Cytometry

Functional Assays

Synonyms
TAPA-1
UniProt
P60033
Entrez Gene
CD81
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0008104 protein localization
GO:0005887 integral to plasma membrane
GO:0006661 phosphatidylinositol biosynthetic process
GO:0008283 cell proliferation
GO:0008284 positive regulation of cell proliferation
GO:0043128 positive regulation of 1-phosphatidylinositol 4-kinase activity
GO:0046813 virion attachment, binding of host cell surface receptor
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050776 regulation of immune response

MCA1847PE

INN1611

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