CD81 antibody | 1D6
Filter by Application:F WB Reset
Mouse anti Human CD81:RPE
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD81 antibody, clone 1D6 recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular leucocytes including activation, proliferation and differentiation.
Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Chimpanzee Sheep Goat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- OCI-LY8 cells aggregated by 5A6 (another CD81 antibody)
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578
- For research purposes only
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for CD81 antibody
Schick, M.R. & Levy, S. (1993) The TAPA-1 molecule is associated on the surface of B cells with HLA-DR molecules.
J Immunol. 151 (8): 4090-7.
Levy, S. et al. (1998) CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system.
Annu Rev Immunol. 16: 89-109.
Flint, M. et al. (1999) Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.
J Virol. 73:6235-44.
Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
Vet Immunol Immunopathol. 119: 123-30.
Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
Vet Immunol Immunopathol. 119: 115-22.
Rohlena, J. et al. (2009) Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion.
Cardiovasc Res. 81: 187-96.
Parthasarathy, V. et al. (2009) Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.
Immunology. 127: 237-48.
Welton, J.L. et al (2010) Proteomics analysis of bladder cancer exosomes.
Mol Cell Proteomics. 9: 1324-38.
View The Latest Product References
Ventress, J.K. et al. (2016) Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes.
PLoS One. 11 (7): e0160387.
Mleczko, J. et al. (2018) Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.
Mol Nutr Food Res. 62 (5)Feb 20 [Epub ahead of print].
- Entrez Gene
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0008104 protein localization
- GO:0005887 integral to plasma membrane
- GO:0006661 phosphatidylinositol biosynthetic process
- GO:0008283 cell proliferation
- GO:0008284 positive regulation of cell proliferation
- GO:0043128 positive regulation of 1-phosphatidylinositol 4-kinase activity
- GO:0046813 virion attachment, binding of host cell surface receptor
- View More GO Terms
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050776 regulation of immune response
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.Yes, sign me up