CD75s antibody | LN-1

Mouse anti Human CD75s

Product Type
Monoclonal Antibody
Clone
LN-1
Isotype
IgM
Specificity
CD75s

Product Code Applications Pack Size List Price Your Price Qty
MCA1481G
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SDS Safety Datasheet SDS
C F P * 0.1 mg loader
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Mouse anti Human CD75s antibody, clone LN-1 recognizes the human CD75s cell surface antigen, an alpha2,6 - sialylated carbohydrate epitope expressed by mature B cells (especially germinal centre B cells), red blood cells and some epithelial cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Mink
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Nuclei from pokeweed mitogen-stimulated PBL
Approx. Protein Concentrations
IgM concentration 0.1mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/10 1/100
Immunohistology - Paraffin 1
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil

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References for CD75s antibody

  1. Epstein, A.L. et al. (1984) Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors.
    J Immunol. 133 (2): 1028-36.
  2. Tanimoto, T. & Ohtsuki,Y. (1996) Evaluation of antibodies reactive with porcine lymphocytes and lymphoma cells in formalin-fixed, paraffin-embedded, antigen-retrieved tissue sections.
    Am J Vet Res. 57 (6): 853-9.
  3. Schwartz-Albiez, R. (2002) Carbohydrate and lectin: sections report in Leucoyte Typing VII: white cell differentiation antigens.
    Edited by Mason, D. et al. Oxford University Press. PP149-164.
  4. Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
    Vet Immunol Immunopathol. 119: 27-37.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

RRID
AB_2194432
UniProt
P15907
Entrez Gene
ST6GAL1
GO Terms
GO:0003835 beta-galactoside alpha-2,6-sialyltransferase activity
GO:0005576 extracellular region
GO:0006959 humoral immune response
GO:0018279 protein N-linked glycosylation via asparagine
GO:0030173 integral to Golgi membrane
GO:0032580 Golgi cisterna membrane
GO:0043687 post-translational protein modification
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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