CD75s antibody | LN-1

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Mouse anti Human CD75s

Product Type: Monoclonal Antibody
Clone: LN-1
Isotype: IgM

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1481G	C F P*			0.1 mg

Mouse anti Human CD75s antibody, clone LN-1 recognizes the human CD75s cell surface antigen, an alpha2,6 - sialylated carbohydrate epitope expressed by mature B cells (especially germinal centre B cells), red blood cells and some epithelial cells.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Pig
Mink

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgM - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.05%

Sodium Azide

Immunogen

Human lymphocytes and SU-DHL4 cell line.

Approx. Protein Concentrations

IgM concentration 0.1mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed until date of expiry. Please see product label.

More Information

UniProt: P15907 Related reagents
Entrez Gene: ST6GAL1 Related reagents
GO Terms: GO:0003835 beta-galactoside alpha-2,6-sialyltransferase activity; GO:0005576 extracellular region; GO:0006959 humoral immune response; GO:0018279 protein N-linked glycosylation via asparagine; GO:0030173 integral to Golgi membrane; GO:0032580 Golgi cisterna membrane; GO:0043687 post-translational protein modification
Regulatory: For research purposes only

Applications of CD75s antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry
Immunohistology - Frozen	1/10	1/100
Immunohistology - Paraffin ¹

¹This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human Tonsil

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgM:FITC	HCA040F	0.1 mg	F
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)	STAR138A	1 ml	C E P WB
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgM Negative Control	MCA692	100 Tests	F

Product Specific References

References for CD75s antibody

Epstein, A.L. et al. (1984) Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors.
J Immunol. 133 (2): 1028-36.
Tanimoto, T. & Ohtsuki,Y. (1996) Evaluation of antibodies reactive with porcine lymphocytes and lymphoma cells in formalin-fixed, paraffin-embedded, antigen-retrieved tissue sections.
Am J Vet Res. 57 (6): 853-9.
Schwartz-Albiez, R. (2002) Carbohydrate and lectin: sections report in Leucoyte Typing VII: white cell differentiation antigens.
Edited by Mason, D. et al. Oxford University Press. PP149-164.
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37.