CD75s antibody | LN-1
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Mouse anti Human CD75s
- Product Type
- Monoclonal Antibody
- Clone
- LN-1
- Isotype
- IgM
- Specificity
- CD75s
Antibody Quality and 
Unrivaled 
| Mouse anti Human CD75s antibody, clone LN-1 recognizes the human CD75s cell surface antigen, an alpha2,6 - sialylated carbohydrate epitope expressed by mature B cells (especially germinal centre B cells), red blood cells and some epithelial cells. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Mink - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Nuclei from pokeweed mitogen-stimulated PBL
- Approx. Protein Concentrations
- IgM concentration 0.1mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunohistology - Frozen | |
1/10 | 1/100 |
| Immunohistology - Paraffin 1 | |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human tonsil
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References for CD75s antibody
-
Epstein, A.L. et al. (1984) Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors.
J Immunol. 133 (2): 1028-36. -
Tanimoto, T. & Ohtsuki,Y. (1996) Evaluation of antibodies reactive with porcine lymphocytes and lymphoma cells in formalin-fixed, paraffin-embedded, antigen-retrieved tissue sections.
Am J Vet Res. 57 (6): 853-9. -
Schwartz-Albiez, R. (2002) Carbohydrate and lectin: sections report in Leucoyte Typing VII: white cell differentiation antigens.
Edited by Mason, D. et al. Oxford University Press. PP149-164. -
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- RRID
- AB_2194432
- UniProt
- P15907
- Entrez Gene
- ST6GAL1
- GO Terms
- GO:0003835 beta-galactoside alpha-2,6-sialyltransferase activity
- GO:0005576 extracellular region
- GO:0006959 humoral immune response
- GO:0018279 protein N-linked glycosylation via asparagine
- GO:0030173 integral to Golgi membrane
- GO:0032580 Golgi cisterna membrane
- GO:0043687 post-translational protein modification
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