Name: Anti Human CD68 Antibody, clone Ki-M7
Brand: Bio-Rad Antibodies
SKU: MCA2375F
Availability: InStock

Mouse anti Human CD68 antibody, clone Ki-M7 recognizes human CD68 an integral membrane glycoprotein of ~110 kDa also known as Macrosialin or Gp110. CD68 is predominantly expressed on the intracellular lysosomes of macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including Acute Myeloid Leukemia.

In immunohistochemistry, CD68 can be used to aid in the identification of blastic NK lymphomas, some B cell lymphomas, and to help diagnose disorders relating to macrophage abnormalities, including malignant histocytosis and Gaucher’s disease.

Clone Ki-M7 has also been reported as being suitable for use in immunoprecipitation.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

African green monkey; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.05% sodium azide (NaN₃)
1% bovine serum albumin
Immunogen: Human lymph node tissue.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature.
Store at 4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹

¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 20μl of the suggested working dilution to label 10⁶ cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸

Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra

Description	Product Code	Applications	Pack Size	Your Price
Mouse IgG1 Negative Control:FITC	MCA928F	F	100 Tests	Log in
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Description	Mouse IgG1 Negative Control:FITC

Description	Product Code	Applications	Pack Size	Your Price
Human Seroblock	BUF070A	F	50 Test	Log in
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Description	Human Seroblock
Human Seroblock	BUF070B	F	200 Test	Log in
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Description	Human Seroblock

Source Reference

Kreipe, H. et al. (1987) Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage and macrophages in human.
J Histochem Cytochem. 35 (10): 1117-26.

References for CD68 antibody

Micklem, K. et al. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
Br J Haematol. 73 (1): 6-11.
Karlsson, K.R. et al. (2008) Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3.
Exp Hematol. 36 (9): 1167-75.
Gottfried, E. et al. (2008) Expression of CD68 in non-myeloid cell types.
Scand J Immunol. 67: 453-63.
Sakakibara, S. et al. (2009) Gene regulation and functional alterations induced by Kaposi's sarcoma-associated herpesvirus-encoded ORFK13/vFLIP in endothelial cells.
J Virol. 83: 2140-53.
Bendelja, K. et al. (2010) Decreased Toll-like receptor 8 expression and lower TNF-α synthesis in infants with acute RSV infection.
Respir Res. 11: 143.
Vergo, S. et al. (2011) Acid-sensing ion channel 1 is involved in both axonal injury and demyelination in multiple sclerosis and its animal model.
Brain. 134 (Pt 2): 571-84.

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CD68 antibody | Ki-M7

Mouse anti Human CD68:FITC