Mouse anti Human CD68 antibody, clone Ki-M7 recognizes human CD68 an integral membrane glycoprotein of ~110 kDa also known as Macrosialin or Gp110. CD68 is predominantly expressed on the intracellular lysosomes of macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including Acute Myeloid Leukemia.
In immunohistochemical studies, CD68 can be used to aid in the identification of blastic NK lymphomas, some B cell lymphomas, and to help diagnose disorders relating to macrophage abnormalities, including malignant histocytosis and Gaucher’s disease.
Clone Ki-M7 has also been reported as being suitable for use in immunoprecipitation studies.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
|African green monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Human lymph node tissue.
- Store at 4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- GO Terms
integral to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD68 antibody
|Flow Cytometry 1
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 20ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
Useful Reagents Available
Application Based External Images
Product Specific References
Kreipe, H. et al. (1987) Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage and macrophages in human.
J Histochem Cytochem. 35 (10): 1117-26.
References for CD68 antibody
Micklem, K. et al. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
Br J Haematol. 73 (1): 6-11.
Karlsson, K.R. et al. (2008) Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3.
Exp Hematol. 36 (9): 1167-75.
Gottfried, E. et al. (2008) Expression of CD68 in non-myeloid cell types.
Scand J Immunol. 67: 453-63.
Sakakibara, S. et al. (2009) Gene regulation and functional alterations induced by Kaposi's sarcoma-associated herpesvirus-encoded ORFK13/vFLIP in endothelial cells.
J Virol. 83: 2140-53.
Bendelja, K. et al. (2010) Decreased Toll-like receptor 8 expression and lower TNF-α synthesis in infants with acute RSV infection.
Respir Res. 11: 143.
Vergo, S. et al. (2011) Acid-sensing ion channel 1 is involved in both axonal injury and demyelination in multiple sclerosis and its animal model.
Brain. 134 (Pt 2): 571-84.
Sadovnikova, E. et al. (2002) The CD68 protein as a potential target for leukaemia-reactive CTL.
Leukemia. 16 (10): 2019-26.
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra