CD68 antibody | Ki-M7
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Mouse anti Human CD68:FITC
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD68 antibody, clone Ki-M7 recognizes human CD68 an integral membrane glycoprotein of ~110 kDa also known as Macrosialin or Gp110. CD68 is predominantly expressed on the intracellular lysosomes of macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including Acute Myeloid Leukemia.
In immunohistochemistry, CD68 can be used to aid in the identification of blastic NK lymphomas, some B cell lymphomas, and to help diagnose disorders relating to macrophage abnormalities, including malignant histocytosis and Gaucher’s disease.
Clone Ki-M7 has also been reported as being suitable for use in immunoprecipitation.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity African green monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.05% sodium azide (NaN3)
1% bovine serum albumin
- Human lymph node tissue.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1|
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 20μl of the suggested working dilution to label 106 cells in 100μl
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Kreipe, H. et al. (1987) Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage and macrophages in human.
J Histochem Cytochem. 35 (10): 1117-26.
References for CD68 antibody
Micklem, K. et al. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
Br J Haematol. 73 (1): 6-11.
Karlsson, K.R. et al. (2008) Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3.
Exp Hematol. 36 (9): 1167-75.
Gottfried, E. et al. (2008) Expression of CD68 in non-myeloid cell types.
Scand J Immunol. 67: 453-63.
Sakakibara, S. et al. (2009) Gene regulation and functional alterations induced by Kaposi's sarcoma-associated herpesvirus-encoded ORFK13/vFLIP in endothelial cells.
J Virol. 83: 2140-53.
Bendelja, K. et al. (2010) Decreased Toll-like receptor 8 expression and lower TNF-α synthesis in infants with acute RSV infection.
Respir Res. 11: 143.
Vergo, S. et al. (2011) Acid-sensing ion channel 1 is involved in both axonal injury and demyelination in multiple sclerosis and its animal model.
Brain. 134 (Pt 2): 571-84.
Sadovnikova, E. et al. (2002) The CD68 protein as a potential target for leukaemia-reactive CTL.
Leukemia. 16 (10): 2019-26.
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