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CD68 antibody | Ki-M7

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Anti Human CD68 Antibody, clone Ki-M7 thumbnail image 1 Anti Human CD68 Antibody, clone Ki-M7 thumbnail image 2
Anti Human CD68 Antibody, clone Ki-M7 gallery image 1
Staining of human peripheral blood monocytes with FITC conjugated Mouse anti Human CD68 (MCA2375F) following permeabilisation with Leucoperm (BUF09)
Anti Human CD68 Antibody, clone Ki-M7 gallery image 2
Published customer image:
FITC conjugated Mouse anti Human CD68 antibody, clone Ki-M7 used to evaluate CD68 expression on stimulated and cultured monocytes by flow cytometry.
Image caption:
Representative figure of unstimulated and CL075-stimulated monocytes stained with CD68 antibody. Fresh PBMC from healthy adult were stimulated with 5 μg/mL CL075 or cultured in media only for 6 h, in the presence of brefeldin A. Intracellular CD68 staining was performed. Staining with isotype matched control antibody is also included.

From: Bendelja K, et al.
Decreased Toll-like receptor 8 expression and lower TNF-α synthesis in infants with acute RSV infection.
Respir Res. 2010;11(1):143.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Human CD68:FITC

Product Type
Monoclonal Antibody
Clone
Ki-M7
Isotype
IgG1
Specificity
CD68

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
MCA2375F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 100 Tests/2ml loader
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Mouse anti Human CD68 antibody, clone Ki-M7 recognizes human CD68 an integral membrane glycoprotein of ~110 kDa also known as Macrosialin or Gp110. CD68 is predominantly expressed on the intracellular lysosomes of macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including Acute Myeloid Leukemia.

In immunohistochemistry, CD68 can be used to aid in the identification of blastic NK lymphomas, some B cell lymphomas, and to help diagnose disorders relating to macrophage abnormalities, including malignant histocytosis and Gaucher’s disease.

Clone Ki-M7 has also been reported as being suitable for use in immunoprecipitation.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
African green monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.05%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human lymph node tissue.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at 4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

Source Reference

  1. Kreipe, H. et al. (1987) Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage and macrophages in human.
    J Histochem Cytochem. 35 (10): 1117-26.

References for CD68 antibody

  1. Micklem, K. et al. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
    Br J Haematol. 73 (1): 6-11.
  2. Karlsson, K.R. et al. (2008) Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3.
    Exp Hematol. 36 (9): 1167-75.
  3. Gottfried, E. et al. (2008) Expression of CD68 in non-myeloid cell types.
    Scand J Immunol. 67: 453-63.
  4. Sakakibara, S. et al. (2009) Gene regulation and functional alterations induced by Kaposi's sarcoma-associated herpesvirus-encoded ORFK13/vFLIP in endothelial cells.
    J Virol. 83: 2140-53.
  5. Bendelja, K. et al. (2010) Decreased Toll-like receptor 8 expression and lower TNF-α synthesis in infants with acute RSV infection.
    Respir Res. 11: 143.
  6. Vergo, S. et al. (2011) Acid-sensing ion channel 1 is involved in both axonal injury and demyelination in multiple sclerosis and its animal model.
    Brain. 134 (Pt 2): 571-84.

Further Reading

  1. Sadovnikova, E. et al. (2002) The CD68 protein as a potential target for leukaemia-reactive CTL.
    Leukemia. 16 (10): 2019-26.

Flow Cytometry

Immunofluorescence

Synonyms
Macrosialin
RRID
AB_2074720
UniProt
P34810
Entrez Gene
CD68
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005765 lysosomal membrane
GO:0010008 endosome membrane
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