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CD66e antibody | C365D3 (NCRC23)

Mouse anti Human CD66e:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
C365D3 (NCRC23)
Isotype
IgG1
Specificity
CD66e

Product Code Applications Pack Size List Price Your Price Qty
MCA1744A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Mouse anti Human CD66e antibody, clone C365D3 (NCRC23) recognizes human Carcinoembryonic antigen-related cell adhesion molecule 5, also known as CD66e, carcinoembryonic antigen, Meconium antigen 100, CEA or CEACAM5. CD66e is a 702 amino acid ~77 kDa GPI anchored membrane protein containing 7 Ig-like domains. Mouse anti Human CD66e antibody, clone C365D3 does not cross-react with normal cross-reacting antigen (CD66c), or with biliary glycoprotein 1 (CD66a) as indicated by binding assays (Price 1988, note: in this study Mouse anti Human CD66e antibody, clone C365D3 is designated as clone 6 (from author)).

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3NSI myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
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Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD66e antibody

  1. Seth, J. et al. (1988) Carcinoembryonic antigen.
    Lancet. 1 (8599): 1399.
  2. Chao, A. et al. (2006) Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression.
    Int J Cancer. 119: 91-8.
  3. Stern-Ginossar, N. et al. (2007) Intercellular transfer of carcinoembryonic antigen from tumor cells to NK cells.
    J Immunol. 179 (7): 4424-34.
  4. Kalinina, T. et al. (2010) Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.
    BMC Cancer.10: 295.
  5. Soucek, K. et al. (2010) Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation.
    Cancer Genet Cytogenet. 197: 107-16.
  6. Ferro, F. et al. (2011) Adipose tissue-derived stem cell in vitro differentiation in a three-dimensional dental bud structure.
    Am J Pathol.178: 2299-310.
  7. Dallas, M.R. et al. (2012) Divergent roles of CD44 and carcinoembryonic antigen in colon cancer metastasis.
    FASEB J. 226: 2648-56.
  8. Domenis, R. et al. (2015) Adipose tissue derived stem cells: in vitro and in vivo analysis of a standard and three commercially available cell-assisted lipotransfer techniques.
    Stem Cell Res Ther. 6: 2.
  9. View The Latest Product References
  10. Wicklein, D. et al. (2018) CEACAM1 promotes melanoma metastasis and is involved in the regulation of the EMT associated gene network in melanoma cells.
    Sci Rep. 8 (1): 11893.
  11. Caponnetto, F. et al. (2020) Human Adipose-Derived Stem Cells in Madelung's Disease: Morphological and Functional Characterization.
    Cells: 10 (1): 44.

Flow Cytometry

Synonyms
CEA
UniProt
P06731
Entrez Gene
CEACAM5
GO Terms
GO:0005887 integral to plasma membrane
GO:0031225 anchored to membrane
GO:0016323 basolateral plasma membrane

MCA1744A647

1807

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