CD66acd antibody | YTH71.3

Rat anti Human CD66acd

Product Type
Monoclonal Antibody
Clone
YTH71.3
Isotype
IgG2a
Specificity
CD66acd

Product Code Applications Pack Size List Price Your Price Qty
MCA1147G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Human CD66acd antibody, clone YTH71.3 recognizes 3 members of the human CD66 family, namely CD66a, also known as Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1 or CEACAM1. Clone YTH71.3 also recognizes CD66c, known as Carcinoembryonic antigen-related cell adhesion molecule 6 or Non-specific crossreacting antigen. In addition the clone also recognizes CD66d which in turn is also known as Carcinoembryonic antigen-related cell adhesion molecule 3 or CGM1 (Watt et al. 1991). CD66 family members are single pass, type 1 membrane glycoproteins bearing a single V-type Ig-like domain and a varying number of C2-type Ig-like domains. Clone YTH71.3 recognizes epitopes in the N-terminal domain of these CD66 family members.

CD66 members are expressed by human neutrophils and cells of the colon where it is down regulated in some cases of colon carcinoma (Neumaier et al. 2012). CD66a is also expressed in the human alveolar adenocarcinoma cell line A549 and may play a role in Moraxella catarrhalis induced apoptosis of pulmonary epithlial cells in diseases including COPD and emphysema (N'Guessan et al. 2007).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin 10 ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
List Price Your Price
loader
Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CD66acd antibody

  1. Watt, S.M. et al. (1991) CD66 identifies a neutrophil-specific epitope within the haematopoietic system that is expressed by members of the carcinoembryonic family of adhesion molecules.
    Blood 78: 63-74.
  2. Skubitz, K.M. et al. (1993) In Leucocyte Typing V. White Cell Differentiation Antigen p. 889 - 899 Volume One.
    Oxford University Press, Oxford.
  3. Jonker, M. et al. (1989) Reactivity of Mab specific for human CD markers with Rhesus monkey leucocytes.
    Leucocyte Typing IV p 1058-1063 Oxford University Press, Oxford.
  4. N'Guessan, P.D. et al. (2007) The UspA1 protein of Moraxella catarrhalis induces CEACAM-1-dependent apoptosis in alveolar epithelial cells.
    J Infect Dis. 195: 1651-60.
  5. Hill, D.J. and Virji, M. (2003) A novel cell-binding mechanism of Moraxella catarrhalis ubiquitous surface protein UspA: specific targeting of the N-domain of carcinoembryonic antigen-related cell adhesion molecules by UspA1.
    Mol Microbiol. 48: 117-29.
  6. Bradley, C.J. et al. (2005) Critical determinants of the interactions of capsule-expressing Neisseria meningitidis with host cells: the role of receptor density in increased cellular targeting via the outer membrane Opa proteins
    Cell Microbiol. 7: 1490-503.

RRID
AB_323396
UniProt
P13688
P40198
P40199
Entrez Gene
CEACAM1
CEACAM3
CEACAM6
GO Terms
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0007267 cell-cell signaling
GO:0031225 anchored to membrane
View more products with CD66 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products