Rat anti Human CD66acd antibody, clone YTH71.3
recognizes 3 members of the human CD66 family, namely CD66a, also known as Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1 or CEACAM1. Clone YTH71.3 also recognizes CD66c, known as Carcinoembryonic antigen-related cell adhesion molecule 6 or Non-specific crossreacting antigen. In addition the clone also recognizes CD66d which in turn is also known as Carcinoembryonic antigen-related cell adhesion molecule 3 or CGM1 (Watt et al. 1991
). CD66 family members are single pass, type 1 membrane glycoproteins bearing a single V-type Ig-like
domain and a varying number of C2-type Ig-like
domains. Clone YTH71.3 recognizes epitopes in the N-terminal domain
of these CD66 family members.
CD66 members are expressed by human neutrophils and cells of the colon where it is down regulated in some cases of colon carcinoma (Neumaier et al. 2012
). CD66a is also expressed in the human alveolar adenocarcinoma cell line A549 and may play a role in Moraxella catarrhalis
induced apoptosis of pulmonary epithlial cells in diseases including COPD and emphysema (N'Guessan et al. 2007
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD66acd antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.