CD59 antibody | YTH53.1

Rat anti Human CD59

Product Type
Monoclonal Antibody
Clone
YTH53.1
Isotype
IgG2b
Specificity
CD59

Product Code Applications Pack Size List Price Your Price Qty
MCA715G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F FN * IF P * WB 0.2 mg loader
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loader
MCA715GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F FN * P * WB 25 µg loader
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loader
Search for Batch Specific Datasheets

Rat anti Human CD59 antibody, clone YTH53.1 recognizes the human CD59 cell surface antigen, also known as MAC-inhibitory protein, 1F5 antigen, 20 kDa homologous restriction factor, MEM43 antigen, membrane attack complex inhibition factor, membrane inhibitor of reactive lysis or protectin. CD59 is produced as a 103 amino acid proprotein, further processed to produce an 18-20 kDa GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes.

Mutation of the CD59 gene can result in the presentation of Hemolytic anemia, CD59-mediated, with or without polyneuropathy (HACD59) a disease characterized by infantile onset of a relapsing-remitting polyneuropathy manifest as limb muscle weakness, and hyporeflexia.

Rat anti Human CD59 antibody, clone YTH53.1 blocks the normal function of CD59 (Walsh et al. 1991).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human Peripheral Blood T cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Functional Assays 1
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 2 1/5 1/10
Western Blotting
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  2. 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Placenta

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References for CD59 antibody

  1. Davies, A. et al. (1989) CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.
    J Exp Med. 170 (3): 637-54.
  2. Walsh, L.A. et al. (1991) Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement.
    Eur J Immunol. 21 (3): 847-50.
  3. Davies, A. et al. (1995) Identification of MIC 11 antigen as an epitope of the CD59 molecule.
    Immunology. 85 (2): 220-7.
  4. Ellison BS et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
    Cell Div. 2: 20.
  5. Ajona, D. et al. (2007) Down-regulation of human complement factor H sensitizes non-small cell lung cancer cells to complement attack and reduces in vivo tumor growth.
    J Immunol. 178 (9): 5991-8.
  6. Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
    Invest Ophthalmol Vis Sci. 50: 3473-81.
  7. Hosokawa, M. et al. (2004) Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs.
    J Neuroinflammation. 1: 17.
  8. Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
    J Leukoc Biol. 86: 155-66.
  9. View The Latest Product References
  10. Zhu, C. et al. (2020) Isatuximab Acts Through Fc-Dependent, Independent, and Direct Pathways to Kill Multiple Myeloma Cells.
    Front Immunol. 11: 1771.

Functional Assays

Immunofluorescence

RRID
AB_566852
UniProt
P13987
Entrez Gene
CD59
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0007166 cell surface receptor linked signaling pathway
GO:0031362 anchored to external side of plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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