CD59 antibody | YTH53.1

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Rat anti Human CD59

Rat anti Human CD59:RPE

Product Type
Monoclonal Antibody
Clone
YTH53.1
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA715GT C F FN* P* WB 25 µg
MCA715G C F FN* IF P* WB 0.2 mg
MCA715PE F 100 Tests
Rat anti Human CD59 antibody, clone YTH53.1 recognizes the human CD59 cell surface antigen, also known as MAC-inhibitory protein, 1F5 antigen, 20 kDa homologous restriction factor, MEM43 antigen, membrane attack complex inhibition factor, membrane inhibitor of reactive lysis or protectin. CD59 is produced as a 103 amino acid proprotein, further processed to produce an 18-20 kDa GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes.

Mutation of the CD59 gene can result in the presentation of Hemolytic anemia, CD59-mediated, with or without polyneuropathy (HACD59) a disease characterized by infantile onset of a relapsing-remitting polyneuropathy manifest as limb muscle weakness, and hyporeflexia.

Rat anti Human CD59 antibody, clone YTH53.1 blocks the normal function of CD59 (Walsh et al. 1991).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Human Peripheral Blood T cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P13987 Related reagents
Entrez Gene
CD59 Related reagents
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0007166 cell surface receptor linked signaling pathway
GO:0031362 anchored to external side of plasma membrane
Regulatory
For research purposes only

Applications of CD59 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Functional Assays 1
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 2 1/5 1/10
Western Blotting
Flow Cytometry Neat
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
  2. 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. We do not recommend using this antibody in lysed whole blood techniques due to inhibition of staining caused by CD59 expression on erythrocytes.
Histology Positive Control Tissue
Placenta

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2b Negative Control MCA6006GA 0.1 mg F
Rat IgG2b Negative Control:RPE MCA6006PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Functional Assays

Immunofluorescence

Product Specific References

References for CD59 antibody

  1. Davies, A. et al. (1989) CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.
    J Exp Med. 170 (3): 637-54.
  2. Walsh, L.A. et al. (1991) Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement.
    Eur J Immunol. 21 (3): 847-50.
  3. Davies, A. et al. (1995) Identification of MIC 11 antigen as an epitope of the CD59 molecule.
    Immunology. 85 (2): 220-7.
  4. Ellison BS et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
    Cell Div. 2: 20.
  5. Ajona, D. et al. (2007) Down-regulation of human complement factor H sensitizes non-small cell lung cancer cells to complement attack and reduces in vivo tumor growth.
    J Immunol. 178 (9): 5991-8.
  6. Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
    Invest Ophthalmol Vis Sci. 50: 3473-81.
  7. Hosokawa, M. et al. (2004) Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs.
    J Neuroinflammation. 1: 17.
  8. Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
    J Leukoc Biol. 86: 155-66.