CD59 antibody | YTH53.1
Rat anti Human CD59
- Product Type
- Monoclonal Antibody
- Clone
- YTH53.1
- Isotype
- IgG2b
- Specificity
- CD59
Antibody Quality and Performance Guarantee
| Rat anti Human CD59 antibody, clone YTH53.1 recognizes the human CD59 cell surface antigen, also known as MAC-inhibitory protein, 1F5 antigen, 20 kDa homologous restriction factor, MEM43 antigen, membrane attack complex inhibition factor, membrane inhibitor of reactive lysis or protectin. CD59 is produced as a 103 amino acid proprotein, further processed to produce an 18-20 kDa GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Mutation of the CD59 gene can result in the presentation of Hemolytic anemia, CD59-mediated, with or without polyneuropathy (HACD59) a disease characterized by infantile onset of a relapsing-remitting polyneuropathy manifest as limb muscle weakness, and hyporeflexia. Rat anti Human CD59 antibody, clone YTH53.1 blocks the normal function of CD59 (Walsh et al. 1991). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human Peripheral Blood T cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Functional Assays 1 | |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 2 | |
1/5 | 1/10 |
| Western Blotting | |
- 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Placenta
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2b Negative Control | ||||||
Source Reference
-
Davies, A. et al. (1989) CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.
J Exp Med. 170 (3): 637-54.
References for CD59 antibody
-
Walsh, L.A. et al. (1991) Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement.
Eur J Immunol. 21 (3): 847-50. -
Davies, A. et al. (1995) Identification of MIC 11 antigen as an epitope of the CD59 molecule.
Immunology. 85 (2): 220-7. -
Hosokawa, M. et al. (2004) Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs.
J Neuroinflammation. 1: 17. -
Ajona, D. et al. (2007) Down-regulation of human complement factor H sensitizes non-small cell lung cancer cells to complement attack and reduces in vivo tumor growth.
J Immunol. 178 (9): 5991-8. -
Ellison BS et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
Cell Div. 2: 20. -
Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
J Leukoc Biol. 86: 155-66. -
Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
Invest Ophthalmol Vis Sci. 50: 3473-81. -
Zhu, C. et al. (2020) Isatuximab Acts Through Fc-Dependent, Independent, and Direct Pathways to Kill Multiple Myeloma Cells.
Front Immunol. 11: 1771.
View The Latest Product References
- RRID
- AB_566852
- UniProt
- P13987
- Entrez Gene
- CD59
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0031362 anchored to external side of plasma membrane
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