CD57 antibody | TB01

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Mouse anti Human CD57

Product Type: Monoclonal Antibody
Clone: TB01
Isotype: IgM
Specificity: CD57

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Secondary Antibodies
References (13)
Applications
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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MCA1305GA Datasheet SDS SDS	C F P *	0.1 mg		Log in
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Anti Human CD57 Antibody, clone TB01 thumbnail image 1

Anti Human CD57 Antibody, clone TB01 thumbnail image 2

Anti Human CD57 Antibody, clone TB01 thumbnail image 3

Anti Human CD57 Antibody, clone TB01 thumbnail image 4

Anti Human CD57 Antibody, clone TB01 gallery image 1 — Figure A. RPE conjugated Mouse anti Human CD56 antibody, clone 123C3 (**MCA2693**) and FITC conjugated Mouse IgM isotype control (**MCA692F**).
Figure B. RPE conjugated Mouse anti Human CD56 antibody, clone 123C3 (**MCA2693**) and FITC conjugated Mouse anti Human CD57 antibody, clone TB01 (**MCA1305F**). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum.
Data acquired on the **ZE5 Cell Analyzer**.

Anti Human CD57 Antibody, clone TB01 gallery image 2 — Staining of paraffin embedded human tonsil with Mouse anti Human CD57 antibody, clone TB01 (**MCA1305GA**).

Anti Human CD57 Antibody, clone TB01 gallery image 3 — **Published customer image:**
FITC conjugated Mouse anti Human CD57 antibody, clone TB01 (**MCA1305F**) used to evaluate CD57 expression on peripheral blood mononuclear cell samples by flow cytometry.
**Image caption:**
Differentiation of infant CD8 T cells during early and chronic infant HIV-1 infection.
Dot plots and histograms show representative phenotyping during the first two years of life in subject B1-093, who was HIV-1 RNA positive at birth. Dot plots show A) CD27 and CD28 and B) CD45RA and CD57 staining. HIV-specific CD8⁺ T cells (gated on tetramer⁺/CD3⁺CD8⁺) are shown as black dot plots overlaid on the overall CD8⁺ subset (gated on CD3⁺CD8⁺), which are shown as density plots in gray. Quadrant statistics are shown below each dot plot, for the overall CD8⁺ (left diagram) and HIV-specific CD8⁺ T cell subsets (right diagram).

From: Slyker JA, John-Stewart GC, Dong T, Lohman-Payne B, Reilly M, Atzberger A, *et al*. (2011)
Phenotypic Characterization of HIV-Specific CD8⁺ T Cells during Early and Chronic Infant HIV-1 Infection.
**PLoS ONE 6(5): e20375**.
doi: 10.1371/journal.pone.0020375
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Human CD57 Antibody, clone TB01 gallery image 4 — **Published customer image:**
FITC conjugated Mouse anti Human CD57 antibody, clone TB01 (**MCA1305F**) used to evaluate CD57 expression on PBMCs by flow cytometry.
**Image caption:**
CD71 and CD57 expression on NK cells. Representative flow cytometry plots from NK cell subsets showing CD71 and CD57 staining in (A) CD56^bright and (B) CD56^dim subsets at 1 and 6 months of age an HIV-infected infant (B1-093).

From: Slyker JA, Lohman-Payne B, John-Stewart GC, Dong T, Mbori-Ngacha D, Tapia K, Atzberger A, Taylor S, Rowland-Jones SL, Blish CA.
The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
**Front Immunol. 2012 Dec 31;3:399**.
doi:10.3389/fimmu.2012.00399.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Mouse anti Human CD57 antibody, clone TB01, recognizes CD57, also known as HNK-1, Leu-7 and NK-1.

CD57 is an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids and chondroitin sulphate proteoglycans. It is expressed on a subset of peripheral blood lymphocytes, including CD8+ T cells and NK cells. The function of CD57 is poorly understood. CD57 binding to laminin and certain selectins suggests it may be involved in cell-cell and cell-matrix interactions.

CD57 is used as a marker for terminally differentiated 'senescent' cells with reduced proliferative capacity and altered functional properties. CD57 expression in CD8+ cells identifies those with reduced proliferative responses to T cell receptor triggering and increased susceptibility to antigen-induced apoptosis. CD57+ T lymphocytes are found at a higher frequency in a variety of diseases. CD57 expression is increased on chronically activated CD8+ T cells in persistent viral infections, such as HIV.

Mouse anti human CD57, clone TB01, is also available as a recombinant antibody (TZA1305).

Target Species: Human
Product Form: Purified IgM - liquid
Preparation: Purified IgM prepared by ion exchange chromatography from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
Immunogen: Human neuroblastoma cells.
Approx. Protein Concentrations: IgM concentration 1mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/50	1/100
Immunohistology - Frozen	1/10	1/100
Immunohistology - Paraffin ¹	1/10	1/50
Immunoprecipitation
Western Blotting

¹ This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl.
Histology Positive Control Tissue: Human tonsil

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References for CD57 antibody

Funaro, A. et al. (1995) Epitope analysis of human CD57 by means of a panel of newly-generated high-affinity murine monoclonal antibodies.
In: Leucocyte Typing V: White Cell Differentiation Antigens.
Funaro, A. et al. (1995) Human CD57, a link molecule between leucocyte and neural cells.
In: Leucocyte Typing V: White Cell Differentiation Antigens.
Slyker, J.A. et al. (2011) Phenotypic Characterization of HIV-Specific CD8 T Cells during Early and Chronic Infant HIV-1 Infection.
PLoS One. 6: e20375.
Nunes, C. et al. (2012) Expansion of a CD8+PD-1+ Replicative Senescence Phenotype in Early Stage CLL Patients Is Associated with Inverted CD4:CD8 Ratios and Disease Progression.
Clin Cancer Res. 18: 678-87.
Khan, N. et al. (2002) Cytomegalovirus seropositivity drives the CD8 T cell repertoire toward greater clonality in healthy elderly individuals.
J Immunol. 169: 1984-92.
Alejenef, A. et al. (2014) Cytomegalovirus drives Vδ2neg &gammaδ T cell inflation in many healthy virus carriers with increasing age.
Clin Exp Immunol. 176 (3): 418-28.
Frahm, M. et al. (2012) CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.
J Immunol. 188: 4289-96.
Wang, Y. et al. (2009) Characteristics of expanded CD4+CD28null T cells in patients with chronic hepatitis B.
Immunol Invest. 38: 434-46.

View The Latest Product References

Lim, H.W. and Kim, C.H. (2007) Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
J Immunol. 179: 7448-56.
Björkström, N.K. et al. (2012) CD8 T cells express randomly selected KIRs with distinct specificities compared with NK cells.
Blood. 120: 3455-65.
Slyker, J.A. et al. (2012) The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Front Immunol. 3: 399.
Perlingeiro Beltrame, M. et al. (2014) Immune reconstitution in patients with Fanconi anemia after allogeneic bone marrow transplantation.
Cytotherapy. 16: 976-89.
Suárez, G.M. et al. (2021) Associations among cytokines, EGF and lymphocyte subpopulations in patients diagnosed with advanced lung cancer.
Cancer Immunol Immunother. 70 (6): 1735-43.