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CD57 antibody | TB01

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Anti Human CD57 Antibody, clone TB01 thumbnail image 1 Anti Human CD57 Antibody, clone TB01 thumbnail image 2 Anti Human CD57 Antibody, clone TB01 thumbnail image 3 Anti Human CD57 Antibody, clone TB01 thumbnail image 4
Anti Human CD57 Antibody, clone TB01 gallery image 1
Figure A. RPE conjugated Mouse anti Human CD56 and FITC conjugated Mouse IgM isotype control (MCA692F). Figure B. RPE conjugated Mouse anti Human CD56 and FITC conjugated Mouse anti Human CD57 (MCA1305F). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Human CD57 Antibody, clone TB01 gallery image 2
Staining of paraffin embedded human tonsil with Mouse anti Human CD57 (MCA1305GA)
Anti Human CD57 Antibody, clone TB01 gallery image 3
Published customer image:
Mouse anti Human CD57 antibody, clone TB01 (MCA1305GA) used to evaluate CD57 expression on PBMC samples by flow cytometry.
Image caption:
Differentiation of infant CD8 T cells during early and chronic infant HIV-1 infection.
Dot plots and histograms show representative phenotyping during the first two years of life in subject B1-093, who was HIV-1 RNA positive at birth. Dot plots show A) CD27 and CD28 and B) CD45RA and CD57 staining. HIV-specific CD8+ T cells (gated on tetramer+/CD3+CD8+) are shown as black dot plots overlaid on the overall CD8+ subset (gated on CD3+CD8+), which are shown as density plots in gray. Quadrant statistics are shown below each dot plot, for the overall CD8+ (left diagram) and HIV-specific CD8+ T cell subsets (right diagram).

From: Slyker JA, John-Stewart GC, Dong T, Lohman-Payne B, Reilly M, Atzberger A, et al. (2011)
Phenotypic Characterization of HIV-Specific CD8+ T Cells during Early and Chronic Infant HIV-1 Infection.
PLoS ONE 6(5): e20375.
doi: 10.1371/journal.pone.0020375
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD57 Antibody, clone TB01 gallery image 4
Published customer image:
FITC conjugated Mouse anti Human CD57 antibody, clone TB01 (MCA1305F) used to evaluate CD57 expression on PBMCs by flow cytometry.
Image caption:
CD71 and CD57 expression on NK cells. Representative flow cytometry plots from NK cell subsets showing CD71 and CD57 staining in (A) CD56bright and (B) CD56dim subsets at 1 and 6 months of age an HIV-infected infant (B1-093).

From: Slyker JA, Lohman-Payne B, John-Stewart GC, Dong T, Mbori-Ngacha D, Tapia K, Atzberger A, Taylor S, Rowland-Jones SL, Blish CA.
The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Front Immunol. 2012 Dec 31;3:399. doi:10.3389/fimmu.2012.00399.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD57:FITC

Mouse anti Human CD57

Product Type
Monoclonal Antibody
Clone
TB01
Isotype
IgM
Product Code Applications Pack Size List Price Quantity
MCA1305F
Datasheet
F
MSDS
100 Tests loader

MCA1305GA
Datasheet
C F P
MSDS
0.1 mg loader

Mouse anti Human CD57 antibody, clone TB01 recognizes CD57, also known as HNK-1, an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is present on a subset of NK and T cells.

Product Details

Target Species
Human
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ion exchange chromatography
Preparation
Purified IgM prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human neuroblastoma cells.
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Approx. Protein Concentrations
IgM concentration 1mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD57 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/10 1/100
Immunohistology - Paraffin 1/10 1/50
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology
*This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining.
Histology Positive Control Tissue
Human tonsil
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgM Negative Control:FITC MCA692F F 0.1 mg loader
Mouse IgM Negative Control MCA692 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Product Specific References

References for CD57 antibody

  1. Funaro, A. et al. (1995) Epitope analysis of human CD57 by means of a panel of newly-generated high-affinity murine monoclonal antibodies.
    In: Leucocyte Typing V: White Cell Differentiation Antigens.
  2. Funaro, A. et al. (1995) Human CD57, a link molecule between leucocyte and neural cells.
    In: Leucocyte Typing V: White Cell Differentiation Antigens.
  3. Slyker, J.A. et al. (2011) Phenotypic Characterization of HIV-Specific CD8 T Cells during Early and Chronic Infant HIV-1 Infection.
    PLoS One. 6: e20375.
  4. Nunes, C. et al. (2012) Expansion of a CD8+PD-1+ Replicative Senescence Phenotype in Early Stage CLL Patients Is Associated with Inverted CD4:CD8 Ratios and Disease Progression.
    Clin Cancer Res. 18: 678-87.
  5. Hutnick, N.A. et al. (2010) Vaccination with Ad5 Vectors Expands Ad5-Specific CD8+ T Cells without Altering Memory Phenotype or Functionality
    PLoS One. 5: e14385.
  6. Khan, N. et al. (2002) Cytomegalovirus seropositivity drives the CD8 T cell repertoire toward greater clonality in healthy elderly individuals.
    J Immunol. 169: 1984-92.
  7. Alejenef, A. et al. (2014) Cytomegalovirus drives Vδ2neg &gammaδ T cell inflation in many healthy virus carriers with increasing age.
    Clin Exp Immunol. 176 (3): 418-28.
  8. Frahm, M. et al. (2012) CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.
    J Immunol. 188: 4289-96.
  9. Wang, Y. et al. (2009) Characteristics of expanded CD4+CD28null T cells in patients with chronic hepatitis B.
    Immunol Invest. 38: 434-46.
  10. Lim, H.W. and Kim, C.H. (2007) Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
    J Immunol. 179: 7448-56.
  11. Björkström, N.K. et al. (2012) CD8 T cells express randomly selected KIRs with distinct specificities compared with NK cells.
    Blood. 120: 3455-65.
  12. Slyker, J.A. et al. (2012) The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
    Front Immunol. 3: 399.
  13. Perlingeiro Beltrame, M. et al. (2014) Immune reconstitution in patients with Fanconi anemia after allogeneic bone marrow transplantation.
    Cytotherapy. 16: 976-89.
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