CD57 antibody | TB01
Mouse anti Human CD57 antibody, clone TB01 (MCA1305GA) used to evaluate CD57 expression on PBMC samples by flow cytometry.
Image caption:
Differentiation of infant CD8 T cells during early and chronic infant HIV-1 infection.
Dot plots and histograms show representative phenotyping during the first two years of life in subject B1-093, who was HIV-1 RNA positive at birth. Dot plots show A) CD27 and CD28 and B) CD45RA and CD57 staining. HIV-specific CD8+ T cells (gated on tetramer+/CD3+CD8+) are shown as black dot plots overlaid on the overall CD8+ subset (gated on CD3+CD8+), which are shown as density plots in gray. Quadrant statistics are shown below each dot plot, for the overall CD8+ (left diagram) and HIV-specific CD8+ T cell subsets (right diagram).
From: Slyker JA, John-Stewart GC, Dong T, Lohman-Payne B, Reilly M, Atzberger A, et al. (2011)
Phenotypic Characterization of HIV-Specific CD8+ T Cells during Early and Chronic Infant HIV-1 Infection.
PLoS ONE 6(5): e20375.
doi: 10.1371/journal.pone.0020375
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Mouse anti Human CD57 antibody, clone TB01 (MCA1305F) used to evaluate CD57 expression on PBMCs by flow cytometry.
Image caption:
CD71 and CD57 expression on NK cells. Representative flow cytometry plots from NK cell subsets showing CD71 and CD57 staining in (A) CD56bright and (B) CD56dim subsets at 1 and 6 months of age an HIV-infected infant (B1-093).
From: Slyker JA, Lohman-Payne B, John-Stewart GC, Dong T, Mbori-Ngacha D, Tapia K, Atzberger A, Taylor S, Rowland-Jones SL, Blish CA.
The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD57 antibody, clone TB01 recognizes CD57, also known as HNK-1, an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is present on a subset of NK and T cells.
Product Details
- Target Species
- Human
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by ion exchange chromatography
- Preparation
- Purified IgM prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human neuroblastoma cells.
- Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgM concentration 1mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD57 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | 1/10 | 1/100 | |
| Immunohistology - Paraffin | 1/10 | 1/50 | |
| Immunoprecipitation | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Immunohistology
- *This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining.
- Histology Positive Control Tissue
- Human tonsil
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
|
|
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
|
|
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
|
|
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgM Negative Control:FITC | MCA692F | F | 0.1 mg |
|
|
| Mouse IgM Negative Control | MCA692 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD57 antibody
-
Funaro, A. et al. (1995) Epitope analysis of human CD57 by means of a panel of newly-generated high-affinity murine monoclonal antibodies.
In: Leucocyte Typing V: White Cell Differentiation Antigens. -
Funaro, A. et al. (1995) Human CD57, a link molecule between leucocyte and neural cells.
In: Leucocyte Typing V: White Cell Differentiation Antigens. -
Slyker, J.A. et al. (2011) Phenotypic Characterization of HIV-Specific CD8 T Cells during Early and Chronic Infant HIV-1 Infection.
PLoS One. 6: e20375. -
Nunes, C. et al. (2012) Expansion of a CD8+PD-1+ Replicative Senescence Phenotype in Early Stage CLL Patients Is Associated with Inverted CD4:CD8 Ratios and Disease Progression.
Clin Cancer Res. 18: 678-87. -
Hutnick, N.A. et al. (2010) Vaccination with Ad5 Vectors Expands Ad5-Specific CD8+ T Cells without Altering Memory Phenotype or Functionality
PLoS One. 5: e14385. -
Khan, N. et al. (2002) Cytomegalovirus seropositivity drives the CD8 T cell repertoire toward greater clonality in healthy elderly individuals.
J Immunol. 169: 1984-92. -
Alejenef, A. et al. (2014) Cytomegalovirus drives Vδ2neg &gammaδ T cell inflation in many healthy virus carriers with increasing age.
Clin Exp Immunol. 176 (3): 418-28. -
Frahm, M. et al. (2012) CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.
J Immunol. 188: 4289-96. -
Wang, Y. et al. (2009) Characteristics of expanded CD4+CD28null T cells in patients with chronic hepatitis B.
Immunol Invest. 38: 434-46. -
Lim, H.W. and Kim, C.H. (2007) Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
J Immunol. 179: 7448-56. -
Björkström, N.K. et al. (2012) CD8 T cells express randomly selected KIRs with distinct specificities compared with NK cells.
Blood. 120: 3455-65. -
Slyker, J.A. et al. (2012) The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Front Immunol. 3: 399. -
Perlingeiro Beltrame, M. et al. (2014) Immune reconstitution in patients with Fanconi anemia after allogeneic bone marrow transplantation.
Cytotherapy. 16: 976-89. -
Suárez, G.M. et al. (2021) Associations among cytokines, EGF and lymphocyte subpopulations in patients diagnosed with advanced lung cancer.
Cancer Immunol Immunother. Jan 02 [Epub ahead of print].
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