CD57 antibody | TB01
Mouse anti Human CD57 antibody, clone TB01 recognizes CD57, also known as HNK-1, an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is present on a subset of NK and T cells.
Product Details
- Target Species
- Human
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by ion exchange chromatography
- Preparation
- Purified IgM prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human neuroblastoma cells.
- Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgM concentration 1mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD57 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1:10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | 1/10 | 1/100 | |
| Immunohistology - Paraffin | 1/10 | 1/50 | |
| Immunoprecipitation | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Immunohistology
- *This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining.
- Histology Positive Control Tissue
- Human tonsil
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | 0.1 mg | F | ||
| Human anti Mouse IgM:HRP | HCA040P | 0.1 mg | E | ||
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | 1 ml | C E P WB | ||
| Goat anti Mouse IgM:RPE | STAR86PE | 0.5 ml | F | ||
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | 1 mg | C E WB | ||
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | 1 mg | E |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgM Negative Control:FITC | MCA692F | 0.1 mg | F | ||
| Mouse IgM Negative Control | MCA692 | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD57 antibody
-
Funaro, A. et al. (1995) Epitope analysis of human CD57 by means of a panel of newly-generated high-affinity murine monoclonal antibodies.
In: Leucocyte Typing V: White Cell Differentiation Antigens. -
Funaro, A. et al. (1995) Human CD57, a link molecule between leucocyte and neural cells.
In: Leucocyte Typing V: White Cell Differentiation Antigens. -
Slyker, J.A. et al. (2011) Phenotypic Characterization of HIV-Specific CD8 T Cells during Early and Chronic Infant HIV-1 Infection.
PLoS One. 6: e20375. -
Cerroni, L. et al. (2004) In: An Illustrated Guide to Skin Lymphoma: Second Edition.
Blackwell Publishing. Oxford. UK
ISBN 104051-1376-6 -
Nunes, C. et al. (2012) Expansion of a CD8+PD-1+ Replicative Senescence Phenotype in Early Stage CLL Patients Is Associated with Inverted CD4:CD8 Ratios and Disease Progression.
Clin Cancer Res. 18: 678-87. -
Hutnick, N.A. et al. (2010) Vaccination with Ad5 Vectors Expands Ad5-Specific CD8+ T Cells without Altering Memory Phenotype or Functionality
PLoS One. 5: e14385. -
Khan, N. et al. (2002) Cytomegalovirus seropositivity drives the CD8 T cell repertoire toward greater clonality in healthy elderly individuals.
J Immunol. 169: 1984-92. -
Alejenef, A. et al. (2014) Cytomegalovirus drives Vδ2neg &gammaδ T cell inflation in many healthy virus carriers with increasing age.
Clin Exp Immunol. 176 (3): 418-28. -
Frahm, M. et al. (2012) CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.
J Immunol. 188: 4289-96. -
Wang, Y. et al. (2009) Characteristics of expanded CD4+CD28null T cells in patients with chronic hepatitis B.
Immunol Invest. 38: 434-46. -
Lim, H.W. and Kim, C.H. (2007) Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
J Immunol. 179: 7448-56. -
Björkström, N.K. et al. (2012) CD8 T cells express randomly selected KIRs with distinct specificities compared with NK cells.
Blood. 120: 3455-65. -
Slyker, J.A. et al. (2012) The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Front Immunol. 3: 399. -
Perlingeiro Beltrame, M. et al. (2014) Immune reconstitution in patients with Fanconi anemia after allogeneic bone marrow transplantation.
Cytotherapy. 16: 976-89.
