CD57 antibody | TB01

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Mouse anti Human CD57:FITC

Mouse anti Human CD57

Product Type
Monoclonal Antibody
Clone
TB01
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1305F F 100 Tests
MCA1305GA C F P 0.1 mg
Mouse anti Human CD57 antibody, clone TB01 recognizes CD57, also known as HNK-1, an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is present on a subset of NK and T cells.

Product Details

Target Species
Human
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ion exchange chromatography
Preparation
Purified IgM prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human neuroblastoma cells.
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Approx. Protein Concentrations
IgM concentration 1mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD57 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1:10
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/10 1/100
Immunohistology - Paraffin 1/10 1/50
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology
*This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining.
Histology Positive Control Tissue
Human tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgM Negative Control:FITC MCA692F 0.1 mg F
Mouse IgM Negative Control MCA692 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD57 antibody

  1. Funaro, A. et al. (1995) Epitope analysis of human CD57 by means of a panel of newly-generated high-affinity murine monoclonal antibodies.
    In: Leucocyte Typing V: White Cell Differentiation Antigens.
  2. Funaro, A. et al. (1995) Human CD57, a link molecule between leucocyte and neural cells.
    In: Leucocyte Typing V: White Cell Differentiation Antigens.
  3. Slyker, J.A. et al. (2011) Phenotypic Characterization of HIV-Specific CD8 T Cells during Early and Chronic Infant HIV-1 Infection.
    PLoS One. 6: e20375.
  4. Cerroni, L. et al. (2004) In: An Illustrated Guide to Skin Lymphoma: Second Edition.
    Blackwell Publishing. Oxford. UK
    ISBN 104051-1376-6
  5. Nunes, C. et al. (2012) Expansion of a CD8+PD-1+ Replicative Senescence Phenotype in Early Stage CLL Patients Is Associated with Inverted CD4:CD8 Ratios and Disease Progression.
    Clin Cancer Res. 18: 678-87.
  6. Hutnick, N.A. et al. (2010) Vaccination with Ad5 Vectors Expands Ad5-Specific CD8+ T Cells without Altering Memory Phenotype or Functionality
    PLoS One. 5: e14385.
  7. Khan, N. et al. (2002) Cytomegalovirus seropositivity drives the CD8 T cell repertoire toward greater clonality in healthy elderly individuals.
    J Immunol. 169: 1984-92.
  8. Alejenef, A. et al. (2014) Cytomegalovirus drives Vδ2neg &gammaδ T cell inflation in many healthy virus carriers with increasing age.
    Clin Exp Immunol. 176 (3): 418-28.
  9. Frahm, M. et al. (2012) CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.
    J Immunol. 188: 4289-96.
  10. Wang, Y. et al. (2009) Characteristics of expanded CD4+CD28null T cells in patients with chronic hepatitis B.
    Immunol Invest. 38: 434-46.
  11. Lim, H.W. and Kim, C.H. (2007) Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
    J Immunol. 179: 7448-56.
  12. Björkström, N.K. et al. (2012) CD8 T cells express randomly selected KIRs with distinct specificities compared with NK cells.
    Blood. 120: 3455-65.
  13. Slyker, J.A. et al. (2012) The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
    Front Immunol. 3: 399.
  14. Perlingeiro Beltrame, M. et al. (2014) Immune reconstitution in patients with Fanconi anemia after allogeneic bone marrow transplantation.
    Cytotherapy. 16: 976-89.