CD49f antibody | NKI-GoH3
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Rat anti Human CD49f:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
|Rat anti Human CD49f antibody, clone NKI-GoH3 recognizes CD49f, also known as the VLA-6 alpha chain. CD49f is a 1107 amino acid ~120 kDa cell surface glycoprotein that forms distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex expressed on various human epithelial cells.
Rat anti Human CD49f antibody, clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common acute lymphoblastic leukemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin.
Rat anti Human CD49f antibody, clone NKI-GoH3 blocks the binding of cells to the E8 fragment of laminin (Sonnenberg et al. 1998).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Dog Pig Cynomolgus monkey Sheep
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- BALB/c mouse mammary tumor cells
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665
- For research purposes only
- 12 months from date of despatch
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or email@example.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106cells in 100ul.
References for CD49f antibody
Sonnenberg, A. et al. (1986) Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin.
J Histochem Cytochem. 34 (8): 1037-46.
Sonnenberg, A. et al. (1987) A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody.
J Biol Chem. 262 (21): 10376-83.
Sonnenberg, A. et al. (1988) Identification and characterization of a novel antigen complex on mouse mammary tumor cells using a monoclonal antibody against platelet glycoprotein Ic.
J Biol Chem. 263 (28): 14030-8.
Sonnenberg, A. et al. (1988) Laminin receptor on platelets is the integrin VLA-6.
Nature. 336 (6198): 487-9.
Hemler, M.E. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures.
J Biol Chem. 263 (16): 7660-5.
Workshop of the 4th International Conference on Human Leucocyte Differentiation Antigens Vienna (1989) Workshop number p 055.
Oxford University Press.
Soligo, D. et al. (1989) Immunohistochemical reacitivy on bone marrow and tissues of anti-VLA antibodies in the platelet panel, in Leucocyte Typing IV: White Cell Differentiation Antigens.
Edited by Knapp, W. et al. Oxford University Press p1029-1032.
Sonnenberg, A. et al. (1990) The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties.
J Cell Sci. 96 ( Pt 2): 207-17.
View The Latest Product References
Sonnenberg, A. et al. (1990) Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragment E8.
J Cell Biol. 110 (6): 2145-55.
Alais, S. et al. (2001) HEMCAM/CD146 downregulates cell surface expression of beta1 integrins.
J Cell Sci. 114: 1847-59.
Dangerfield, J. et al. (2002) PECAM-1 (CD31) homophilic interaction up-regulates alpha6beta1 on transmigrated neutrophils in vivo and plays a functional role in the ability of alpha6 integrins to mediate leukocyte migration through the perivascular basement membrane.
J Exp Med. 196: 1201-11.
Le Bellego, F. et al. (2005) Cytoskeleton reorganization mediates alpha6beta1 integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells.
Reprod Biol Endocrinol. 3: 19.
Anderson, C. et al. (2009) Sonic hedgehog-dependent synthesis of laminin alpha1 controls basement membrane assembly in the myotome.
Development. 136: 3495-504.
Jensen, K.B. et al. (2010) Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis.
Nat Protoc. 5 (5): 898-911.
da Silva, R.G. et al. (2010) Endothelial alpha3beta1-integrin represses pathological angiogenesis and sustains endothelial-VEGF.
Am J Pathol. 177: 1534-48.
Collins, C.A. et al. (2011) Reprogramming adult dermis to a neonatal state through epidermal activation of β-catenin
Schäfer, G. et al. (2013) The role of inflammation in HPV infection of the Oesophagus.
BMC Cancer. 13: 185.
Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Vet Parasitol. 220: 33-45.
Mastrogiannaki, M. et al. (2016) β-Catenin Stabilization in Skin Fibroblasts Causes Fibrotic Lesions by Preventing Adipocyte Differentiation of the Reticular Dermis.
J Invest Dermatol. 136 (6): 1130-42.
Haining, E.J. et al. (2017) Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation.
Platelets. 28 (7): 629-42.
Peuhu. E, et al. (2017) Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice
PLOS ONE. 12 (10): e0186628.
Rayagiri, S.S. et al. (2018) Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal.
Nat Commun. 9 (1): 1075.
Loureiro, J. et al. (2019) Conjugation of the T1 sequence from CCN1 to fibrin hydrogels for therapeutic vascularization.
Mater Sci Eng C Mater Biol Appl. 104: 109847.
Ikeda, A. et al. (2020) Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells.
Sci Rep. 10 (1): 19959.
Lorenzo-Martín, L.F. et al. (2022) The Rho guanosine nucleotide exchange factors Vav2 and Vav3 modulate epidermal stem cell function.
Oncogene. May 09 [Epub ahead of print].
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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