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CD45RB antibody | MEM-55

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Anti Human CD45RB Antibody, clone MEM-55 thumbnail image 1 Anti Human CD45RB Antibody, clone MEM-55 thumbnail image 2 Anti Human CD45RB Antibody, clone MEM-55 thumbnail image 3
Anti Human CD45RB Antibody, clone MEM-55 gallery image 1
Figure A. FITC conjugated Rat anti Human CD8 (MCA351F) and isotype control (MCA928) detected with Goat anti Mouse IgG: RPE (STAR76). Figure B. FITC conjugated Rat anti Human CD8 (MCA351F) and Mouse anti Human CD45RB (MCA1114) detected with Goat anti Mouse IgG RPE (STAR76). All experiments performed on red cell lysed human blood gated on single lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RB Antibody, clone MEM-55 gallery image 2
Figure A. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB) and APC conjugated Mouse IgG1 isotype control (MCA928APC). Figure B. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB) and APC conjugated Mouse anti Human CD45RB (MCA1114APC). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RB Antibody, clone MEM-55 gallery image 3
Figure A. RPE conjugated Mouse anti Human CD4 (MCA1267PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Human CD4 (MCA1267PE) and FITC conjugated Mouse anti Human CD45RB (MCA1114F). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD45RB:APC

Product Type
Monoclonal Antibody
Clone
MEM-55
Isotype
IgG1
Specificity
CD45RB

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Product Code Applications Pack Size List Price Your Price Qty
MCA1114APC
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
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Mouse anti Human CD45RB antibody, clone MEM-55 recognizes the isoform of the leucocyte common antigen encoded by exon B and stains B cells, most T cells and monocytes.

Mouse anti Human CD45RB antibody, clone MEM-55 immunoprecipitates bands of 190, 200 and 205 kDa.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Horse
Goat
Llama
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
APC 650 661
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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References for CD45RB antibody

  1. Blaylock, M.G. et al. (2003) Eosinophils from patients with asthma express higher levels of the pan-leucocyte receptor CD45 and the isoform CD45RO.
    Clin Exp Allergy. 33: 936-41.
  2. Windhagen, A. et al. (2007) Altered CD45 isoform expression in C77G carriers influences cytokine responsiveness and adhesion properties of T cells.
    Clin Exp Immunol. 150: 509-17.
  3. Ibrahim, S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes.
    Vet Immunol Immunopathol. 119: 63-80.
  4. Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
    Vet Immunol Immunopathol. 119: 123-30.
  5. Ponchel, F. et al. (2014) An immunological biomarker to predict MTX response in early RA.
    Ann Rheum Dis. 73 (11): 2047-53.
  6. Khan, A.A. et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
    J Virol. 89 (13): 6619-32.
  7. Hunt, L. et al. (2016) T cell subsets: an immunological biomarker to predict progression to clinical arthritis in ACPA-positive individuals.
    Ann Rheum Dis. 75 (10): 1884-9.

RRID
AB_1125266
UniProt
P08575
Entrez Gene
PTPRC
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0019901 protein kinase binding
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus

MCA1114APC

149605

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