CD45 antibody | F10-89-4

Mouse anti Human CD45:StarBright Violet 570

Product Type
Monoclonal Antibody
Clone
F10-89-4
Isotype
IgG2a
Specificity
CD45

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MCA87SBV570
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Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms.

Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.

Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes.

Target Species
Human
Product Form
Purified IgG conjugated to StarBright Violet 570 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Human T lymphocytes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 570 402 570
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for CD45 antibody

  1. Dalchau, R. et al. (1980) Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat.
    Eur J Immunol. 10 (10): 737-44.
  2. Quenby, S et al. (1999) Pre-implantation endometrial leukocytes in women with recurrent miscarriage.
    Human Reprod. 14(9):2386-2391.
  3. Hauser, P.V. et al. (2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.
    Am J Pathol. 177: 2011-21.
  4. Mallam, E. et al. (2010) Characterization of in vitro expanded bone marrow-derived mesenchymal stem cells from patients with multiple sclerosis.
    Mult Scler. 16: 909-18.
  5. Marrinucci, D. et al. (2010) Cytomorphology of circulating colorectal tumor cells:a small case series.
    J Oncol. 2010: 861341.
  6. Kazane, S.A. et al. (2012) Site-specific DNA-antibody conjugates for specific and sensitive immuno-PCR.
    Proc Natl Acad Sci U S A. 109: 3731-6.
  7. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One. 7: e35577.
  8. Sadarangani, A. et al. (2015) GLI2 inhibition abrogates human leukemia stem cell dormancy.
    J Transl Med. 13: 98.
  9. View The Latest Product References
  10. Gunawardene, P. et al. (2015) Association Between Circulating Osteogenic Progenitor Cells and Disability and Frailty in Older Persons: The Nepean Osteoporosis and Frailty Study.
    J Gerontol A Biol Sci Med Sci. pii: glv190.
  11. Gogoi P et al. (2016) Development of an Automated and Sensitive Microfluidic Device for Capturing and Characterizing Circulating Tumor Cells (CTCs) from Clinical Blood Samples.
    PLoS One. 11 (1): e0147400.
  12. Spaas, J.H. et al. (2013) Culture and characterisation of equine peripheral blood mesenchymal stromal cells.
    Vet J. 195 (1): 107-13.
  13. Gomiero, C. et al. (2016) Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology.
    Vet Res Commun. 40 (1): 39-48.
  14. De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
    Cytometry A. 81 (4): 312-23.
  15. Bianchessi, M. et al. (2016) Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis.
    Stem Cells Int. 2016: 9364974.
  16. Mohamed Suhaimi, N.A. et al. (2015) Non-invasive sensitive detection of KRAS and BRAF mutation in circulating tumor cells of colorectal cancer patients.
    Mol Oncol. 9 (4): 850-60.
  17. Ruiz, C. et al. (2015) Limited genomic heterogeneity of circulating melanoma cells in advanced stage patients.
    Phys Biol. 12 (1): 016008.
  18. Branly, T. et al. (2017) Characterization and use of Equine Bone Marrow Mesenchymal Stem Cells in Equine Cartilage Engineering. Study of their Hyaline Cartilage Forming Potential when Cultured under Hypoxia within a Biomaterial in the Presence of BMP-2 and TGF-β1.
    Stem Cell Rev. Jun 09 [Epub ahead of print].
  19. GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.
  20. Shishido, S.N. et al. (2019) Circulating tumor cells as a response monitor in stage IV non-small cell lung cancer.
    J Transl Med. 17 (1): 294.
  21. Welter, L. et al. (2020) Treatment response and tumor evolution: Lessons from an extended series of multi-analyte liquid biopsies in a metastatic breast cancer patient.
    Cold Spring Harb Mol Case Stud. Nov 17 [Epub ahead of print].
  22. Ndacayisaba, L.J. et al. (2022) Enrichment-Free Single-Cell Detection and Morphogenomic Profiling of Myeloma Patient Samples to Delineate Circulating Rare Plasma Cell Clones
    Curr Oncol. 29 (5): 2954-72.
  23. Shishido, S.N. et al. (2022) Liquid Biopsy Landscape in Patients with Primary Upper Tract Urothelial Carcinoma.
    Cancers (Basel). 14 (12): 3007.
  24. Chai, S. et al. (2022) Identification of epithelial and mesenchymal circulating tumor cells in clonal lineage of an aggressive prostate cancer case.
    NPJ Precis Oncol. 6 (1): 41.

Flow Cytometry

Immunofluorescence

Synonyms
LCA
UniProt
P08575
Entrez Gene
PTPRC
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0019901 protein kinase binding
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
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