CD24 antibody | SN3

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Mouse anti Human CD24:StarBright Blue 700

Product Type: Monoclonal Antibody
Clone: SN3
Isotype: IgG1
Specificity: CD24

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References (7)
Applications
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Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA1379SBB700 Datasheet SDS SDS	F *	100 Tests/0.5ml		Log in
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Anti Human CD24 Antibody, clone SN3 gallery image 1 — Figure A. FITC conjugated Mouse anti Human CD66b antibody, clone 80H3 and RPE conjugated Mouse IgG1 isotype control (**MCA928PE**).
Figure B. FITC conjugated Mouse anti Human CD66b antibody, clone 80H3 and RPE conjugated Mouse anti Human CD24 antibody, clone SN3 (**MCA1379PE**). All experiments performed on red cell lysed human blood gated on myeloid cells in the presence of 10% human serum.
Data acquired on the **ZE5 Cell Analyzer**.

Anti Human CD24 Antibody, clone SN3 gallery image 2 — Figure A. APC conjugated Mouse anti Human CD45 antibody, clone F10-89-4 (**MCA87APC**) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**).
Figure B. APC conjugated Mouse anti Human CD45 antibody, clone F10-89-4 (**MCA87APC**) and FITC conjugated Mouse anti Human CD24 antibody, clone SN3 (**MCA1379F**). All experiments performed on red cell lysed human blood gated on live myeloid cells in the presence of 10% human serum.
Data acquired on the **ZE5 Cell Analyzer**.

Anti Human CD24 Antibody, clone SN3 gallery image 3 — Figure A. FITC conjugated Mouse anti Human CD66b antibody clone 80H3 and Alexa Fluor^® 647 conjugated Mouse IgG1 isotype control (**MCA928A647**).
Figure B. FITC conjugated Mouse anti Human CD66b antibody clone 80H3 and Alexa Fluor^® 647 conjugated Mouse anti Human CD24 antibody, clone SN3 (**MCA1379A647**). All experiments performed on human blood gated on live single granulocytes, in the presence of 10% human serum.
Data acquired on the **ZE5 Cell Analyzer**.

Anti Human CD24 Antibody, clone SN3 gallery image 4 — Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 antibody, clone LT19 (**MCA1940SBUV400**).
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 antibody, clone LT19 (**MCA1940SBUV400**) and StarBright Violet 790 conjugated Mouse anti Human CD24 antibody, clone SN3 (**MCA1379SBV790**). All experiments performed on human PBMCs gated on live single lymphocytes, in the presence of 10% human serum.
Data acquired on the **ZE5 Cell Analyzer**.

Anti Human CD24 Antibody, clone SN3 gallery image 5 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 (**MCA87SBV440**).
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 (**MCA87SBV440**) and StarBright Blue 700 conjugated Mouse anti Human CD24 antibody, clone SN3(**MCA1379SBB700**).
All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the **ZE5 Cell Analyzer**.

Anti Human CD24 Antibody, clone SN3 gallery image 6 — Figure A. StarBright Red 670 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 (**MCA87SBR670**).
Figure B. StarBright Red 670 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 (**MCA87SBR670**) and StarBright Violet 570 conjugated Mouse anti Human CD24 antibody, clone SN3 (**MCA1379SBV570**).
All experiments performed on red cell lysed human blood gated on live single granulocytes, in the presence of 10% human serum. Data acquired on the **ZE5 Cell analyser**.

Anti Human CD24 Antibody, clone SN3 gallery image 7 — Figure A. StarBright Red 670 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 (**MCA87SBR670**).
Figure B. StarBright Red 670 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 (**MCA87SBR670**) and StarBright Violet 440 conjugated Mouse anti Human CD24 antibody, clone SN3 (**MCA1379SBV440**). All experiments performed on red blood lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum.
Data acquired on the **ZE5 Cell analyser**.

Anti Human CD24 Antibody, clone SN3 gallery image 8 — Figure A. FITC conjugated Mouse anti Human CD66b antibody, clone 80H3.
Figure B. FITC conjugated Mouse anti Human CD66b antibody, clone 80H3 and StarBright Violet 610 conjugated Mouse anti Human CD24 antibody, clone SN3 (**MCA1379SBV610**). All experiments performed on red blood lysed human blood gated on live single granulocytes, in the presence of 10% human serum.
Data acquired on the **ZE5 Cell analyser**.

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Mouse anti Human CD24 antibody, clone SN3 recognizes the human CD24 cell surface antigen, a heavily glycosylated molecule of ~35-45 kDa. CD24 is expressed by granulocytes and B lymphocytes, and by some activated T cells and T cell malignancies.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Chimpanzee; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG conjugated to StarBright Blue 700 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen: Glycoproteins purified from human NALM-1 cell line
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of a mouse myeloma cell line
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright Blue 700 473 703
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat

¹Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Source Reference

Barcos, M. et al. (1986) Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3.
Hematol Oncol. 4 (4): 251-9.

References for CD24 antibody

Fukukawa, T. et al. (1986) New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non-T leukemia cells.
Exp Hematol. 14 (9): 850-5.
Ohyama, M. et al. (2006) Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
J Clin Invest. 116:249-60.
Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
J Histochem Cytochem. 57 (12): 1159-67.
Sallustio, F. et al. (2010) TLR2 plays a role in the activation of human resident renal stem/progenitor cells.
FASEB J. 24 (2): 514-25.
Kanderová, V. et al. (2010) Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells.
Exp Hematol. 38: 653-660.e1.
Franz, C. et al. (2016) Extracellular vesicles in human follicular fluid do not promote coagulation.
Reprod Biomed Online. 33 (5): 652-5.

Flow Cytometry

UniProt: P25063
Entrez Gene: CD24
GO Terms: GO:0005886 plasma membrane; GO:0001666 response to hypoxia; GO:0001775 cell activation; GO:0001959 regulation of cytokine-mediated signaling pathway; GO:0002237 response to molecule of bacterial origin; GO:0002768 immune response-regulating cell surface receptor signaling pathway; GO:0004871 signal transducer activity; GO:0016055 Wnt receptor signaling pathway; GO:0007204 elevation of cytosolic calcium ion concentration; View More GO Terms; GO:0007411 axon guidance; GO:0008629 induction of apoptosis by intracellular signals; GO:0009986 cell surface; GO:0031225 anchored to membrane; GO:0016337 cell-cell adhesion; GO:0016477 cell migration; GO:0019901 protein kinase binding; GO:0030296 protein tyrosine kinase activator activity; GO:0030856 regulation of epithelial cell differentiation; GO:0031295 T cell costimulation; GO:0032597 B cell receptor transport into membrane raft; GO:0032600 chemokine receptor transport out of membrane raft; GO:0032913 negative regulation of transforming growth factor-beta3 production; GO:0042104 positive regulation of activated T cell proliferation; GO:0042632 cholesterol homeostasis; GO:0043406 positive regulation of MAP kinase activity; GO:0043408 regulation of MAPKKK cascade; GO:0043627 response to estrogen stimulus; GO:0045121 membrane raft; GO:0045730 respiratory burst