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CD38 antibody | AT13/5

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Anti Human CD38 Antibody, clone AT13/5 thumbnail image 1 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 2 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 3 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 4 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 5 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 6 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 7 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 8 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 9 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 10 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 11 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 12 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 13 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 14 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 15 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 16 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 17 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 18 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 19 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 20 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 21 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 22 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 23 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 24 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 25 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 26 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 27 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 28 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 29 Anti Human CD38 Antibody, clone AT13/5 thumbnail image 30
Anti Human CD38 Antibody, clone AT13/5 gallery image 1
Figure A. RPE-Cy5 tandem conjugated Mouse anti Human CD3 (MCA463C) and A488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. RPE-Cy5 tandem conjugated Mouse anti Human CD3 (MCA463C) and A488 conjugated Mouse anti Human CD38 (MCA1019A488). All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD38 Antibody, clone AT13/5 gallery image 2
Published customer image:
Mouse anti Human CD38 antibody, clone AT13/5 (MCA1019PE) used to evaluate CD38 expression on peripheral blood mononuclear cells by flow cytometry.
Image caption:
CD38brightHLA-CD69+ NK cells. Representative flow cytometry plots from NK cell subsets showing identification of CD38brightCD69+ cells in (A) CD56bright and (B) CD56dim subsets at 1 and 6 months of age an HIV-infected infant (B1-093). (C) Plots show individual infant trajectories as connected lines of different colors, with overlaid linear mixed models (LMM). LMM were used to determine whether slope (change in % of cells/month) was affected by HIV infection status; where slopes are not different, the slope representing the combined data of all infants is shown to the box on the right. The mean difference across HIV groups is derived from the beta coefficient for [HIV status] as described in the Materials and Methods, and represents the % increase in cells per HIV category from HIV-unexposed to HIV-exposed uninfected to HIV-infected. Slopes for each HIV infection group are also presented separately within each graph.

From: Slyker JA, Lohman-Payne B, John-Stewart GC, Dong T, Mbori-Ngacha D, Tapia K, Atzberger A, Taylor S, Rowland-Jones SL, Blish CA.
The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Front Immunol. 2012 Dec 31;3:399.
doi: 10.3389/fimmu.2012.00399.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD38 Antibody, clone AT13/5 gallery image 3
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and SBV515 conjugated Mouse anti Human CD38 (MCA1019SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 4
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 670 conjugated Mouse anti Human CD38 (MCA1019SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 5
Figure A. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C). Figure B. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C) and StarBright Violet 440 conjugated Rat anti Human CD38 (MCA1019SBV440). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 6
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515) and StarBright Violet 610 conjugated Mouse anti Human CD38 (MCA1019SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 7
Figure A. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C). Figure B. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C) and StarBright Violet 570 conjugated Mouse anti Human CD38 (MCA1019SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 8
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Violet 710 conjugated Mouse anti Human CD38 (MCA1019SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 9
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 790 conjugated Mouse anti Human CD38 (MCA1019SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 10
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and StarBright Violet 475 conjugated Mouse anti Human CD38 (MCA1019SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 11
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610) and StarBright UltraViolet 400 conjugated Mouse anti Human CD38 (MCA1019SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 12
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 760 conjugated Mouse anti Human CD38 (MCA1019SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 13
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and StarBright UltraViolet 665 conjugated Mouse anti Human CD38 (MCA1019SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 14
Figure A. StarBright Violet 670 conjugated Mouse anti Human CD3 (MCA463SBV670). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD3 (MCA463SBV670) and StarBright UltraViolet 445 conjugated Mouse anti Human CD38 (MCA1019SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 15
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610) and StarBright UltraViolet 795 conjugated Mouse anti human CD38 (MCA1019SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 16
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and StarBright UltraViolet 510 conjugated Mouse anti Human CD38 (MCA1019SBUV510). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 17
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). and StarBright UltraViolet 575 conjugated Mouse anti Human CD38 (MCA1019SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 18
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and StarBright UltraViolet 605 conjugated Mouse anti Human CD38 (MCA1019SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 19
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and StarBright UltraViolet 605 conjugated Mouse anti Human CD38 (MCA1019SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 20
Figure A. RPE-Cy5.5 conjugated Mouse anti Human CD3. Figure B. RPE-Cy5.5 conjugated Mouse anti Human CD3 and StarBright Blue 580 conjugated Mouse anti Human CD38 (MCA1019SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 21
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Blue 615 conjugated Mouse anti Human CD38 (MCA1019SBB615). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 22
Figure A. StarBright UltraViolet 510 conjugated Mouse anti Human CD3 (MCA463SBUV510). Figure B. StarBright UltraViolet 510 conjugated Mouse anti Human CD3 (MCA463SBUV510) and StarBright Blue 675 conjugated Mouse anti Human CD38 (MCA1019SBB675). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 23
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Blue 765 conjugated Mouse anti Human CD38 (MCA1019SBB765). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 24
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515) and StarBright Blue 810 conjugated Mouse anti Human CD38 (MCA1019SBB810). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 25
Figure A. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765). Figure B. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765) and StarBright Yellow 575 conjugated Mouse anti Human CD38 (MCA1019SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 26
Figure A. StarBright Blue 580 conjugated Mouse anti Human CD3 (MCA463SBB580). Figure B. StarBright Blue 580 conjugated Mouse anti Human CD3 (MCA463SBB580) and StarBright Yellow 720 conjugated Mouse anti Human CD38 (MCA1019SBY720). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 27
Figure A. StarBright Blue 615 conjugated Mouse anti Human CD3 (MCA463SBB615). Figure B. StarBright Blue 615 conjugated Mouse anti Human CD3 (MCA463SBB615) and StarBright Yellow 665 conjugated Mouse anti Human CD38 (MCA1019SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 28
Figure A. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765). Figure B. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765) and StarBright Yellow 605 conjugated Mouse anti Human CD38 (MCA1019SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 29
Figure A. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright Ultra Violet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Yellow 800 conjugated Mouse anti Human CD38 (MCA1019SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD38 Antibody, clone AT13/5 gallery image 30
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610) and StarBright Red 775 conjugated Mouse anti Human CD38 (MCA1019SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD38:StarBright UltraViolet 605

Product Type
Monoclonal Antibody
Clone
AT13/5
Isotype
IgG1
Specificity
CD38

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Product Code Applications Pack Size List Price Your Price Qty
MCA1019SBUV605
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SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
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Mouse anti Human CD38 antibody, clone AT13/5 recognizes CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1 or T10. CD38 is a 300 amino acid ~45 kDa single pass type II transmembrane glycoprotein and Mouse anti Human CD38 antibody, clone AT13/5 has a reactivity pattern consistent with other antibodies of the CD38 cluster. CD38 is expressed by plasma cells, monocytes, early lymphoid cells and activated T cells.

CD38 is widely used to study the processes of B and T differentiation and activation. CD38 is a multifunctional enzyme involved in calcium signaling and nicotinamide adenine dinucleotide (NAD+) metabolism (de Oliveira et al. 2018).

Target Species
Human
Product Form
Purified IgG conjugated to StarBright UltraViolet 605 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin.
0.1% Pluronic F68.
0.1% PEG 3350.
0.05% Tween 20.
Immunogen
Namalwa human B-cell line.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright UltraViolet 605 340 609
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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Source Reference

  1. Ellis, J.H. et al. (1995) Engineered anti-CD38 monoclonal antibodies for immunotherapy of multiple myeloma.
    J Immunol. 155 (2): 925-37.

References for CD38 antibody

  1. Corcione, A. et al. (2009) CX3CR1 is expressed by human B lymphocytes and mediates [corrected] CX3CL1 driven chemotaxis of tonsil centrocytes.
    PLoS One. 4: e8485.
  2. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. 12: 2.
  3. Tipping, A.J. et al. (2009) High GATA-2 expression inhibits human hematopoietic stem and progenitor cell function by effects on cell cycle
    Blood. 113: 2661-72.
  4. Slyker, J.A. et al. (2012) Acute cytomegalovirus infection is associated with increased frequencies of activated and apoptosis-vulnerable T cells in HIV-1-infected infants.
    J Virol. 86 (20): 11373-9.
  5. Slyker, J.A. et al. (2012) The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
    Front Immunol. 3: 399.
  6. Kelleher, M. et al. (2011) Comparative Kinetics of Immune Responses and Changes in Cellular Sub-Sets Detected in Colorectal Cancer Patients Vaccinated with MVA-5T4 (TroVax) Administered Alongside Two Different Chemotherapy Regimens
    J Cancer Therapy. 02 (01): 54-64.

Further Reading

  1. Malavasi, F. et al. (1994) Human CD38: a glycoprotein in search of a function.
    Immunol Today. 15 (3): 95-7.

Flow Cytometry

UniProt
P28907
Entrez Gene
CD38
GO Terms
GO:0005886 plasma membrane
GO:0003953 NAD+ nucleosidase activity
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005488 binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0042493 response to drug
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030890 positive regulation of B cell proliferation
GO:0043066 negative regulation of apoptosis
GO:0050853 B cell receptor signaling pathway

MCA1019SBUV605

100006782

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