CD33 antibody | WM53

Mouse anti Human CD33:StarBright Violet 610

Product Type
Monoclonal Antibody
Clone
WM53
Isotype
IgG1
Specificity
CD33

Product Code Applications Pack Size List Price Your Price Qty
MCA1271SBV610
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Mouse anti Human CD33 antibody, clone WM53 recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin.

Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets.

Target Species
Human
Product Form
Purified IgG conjugated to StarBright Violet 610 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Human AML cells
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 610 402 607
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for CD33 antibody

  1. Favaloro, E.J. et al. (1987) Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, gp67 (CD-33).
    Dis Markers. 5 (4): 215-25.
  2. Favaloro, E.J. et al. (1988) Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33.
    Br J Haematol. 69 (2): 163-71.
  3. Hernández-Caselles, T. et al. (2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
    J Leukoc Biol. 79: 46-58.
  4. Biedermann, B. et al. (2006) Analysis of the CD33-related siglec family reveals that Siglec-9 is an endocytic receptor expressed on subsets of acute myeloid leukemia cells and absent from normal hematopoietic progenitors.
    Leuk Res. 31: 211-20.
  5. Lajaunias, F. et al. (2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling.
    Eur J Immunol. 35: 243-51.
  6. Pietschmann, P. et al. (2000) Surface markers and transendothelial migration of dendritic cells from elderly subjects.
    Exp Gerontol. 35: 213-24.
  7. Favaloro, E.J. et al. (1993) Differential expression of surface antigens on activated endothelium.
    Immunol Cell Biol. 71:571-81.
  8. Yasukawa, T. et al. (2012) Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis.
    Anal Chem. 84 (20): 8830-6.
  9. View The Latest Product References
  10. Hu, Z. et al. (2016) Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibody
    J Wuhan Univ of Technol-Mater. Sci. Ed. 31 (2): 446-53.
  11. Dahl C et al. (2004) Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells cultured in vitro.
    Allergy. 59 (10): 1087-96.
  12. Vamvakopoulos, J.E. & Green, C. (2003) HMG-CoA reductase inhibition aborts functional differentiation and triggers apoptosis in cultured primary human monocytes: a potential mechanism of statin-mediated vasculoprotection.
    BMC Cardiovasc Disord. 3: 6.
  13. Vamvakopoulos, J. et al. (2002) Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.
    Eur J Immunol. 32 (10): 2988-96.
  14. Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
    Blood. 106 (10): 3567-74.
  15. McCormack E et al. (2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.
    Blood. 121 (7): e34-42.
  16. Hernández-Caselles T et al. (2019) CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
    J Immunol Res. 2019: 6032141.

UniProt
P20138
Entrez Gene
CD33
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0007267 cell-cell signaling
GO:0008285 negative regulation of cell proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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