CD324 antibody | AB04/4G6

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Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) gallery image 1 — A431 cells stained with 5μg/ml Mouse anti CD324 antibody (**VMA00502**, green). Hoechst was used as nuclear stain (blue) and imaged with a 20x objective.

Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates probed with CD324 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to CD324 (molecular weight 120 kDa).

Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) gallery image 3 — Western blot analysis of CD324 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with Mouse anti CD324 antibody (**VMA00502**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and visualized on the ChemiDoc MP with a 29 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.

Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) gallery image 4 — Normalized signal intensity of CD324 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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IF WB

Mouse anti CD324

Product Type: PrecisionAb Monoclonal
Clone: AB04/4G6
Isotype: IgG2b
Format: Purified
Specificity: CD324

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00502 Datasheet Datasheet SDS SDS	IF WB	100 µl		Log in	Get Quote
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Mouse anti Human CD324 antibody recognizes CD324, also known as cadherin-1, CAM 120/80, E-cadherin, epithelial cadherin or uvomorulin.

The CDH1 gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in CDH1 gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites (provided by RefSeq, Jul 2008).

Mouse anti Human CD324 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CD324 detects a band of approximately 120 kDa in LNCaP cell lysate.
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli derived recombinant protein corresponding to amino acids 338-621 of human CD324
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P12830
Entrez Gene: CDH1
GO Terms: GO:0016021 integral to membrane; GO:0050839 cell adhesion molecule binding; GO:0005913 cell-cell adherens junction; GO:0005925 focal adhesion; GO:0006921 cellular component disassembly involved in apoptosis; GO:0007156 homophilic cell adhesion; GO:0015629 actin cytoskeleton; GO:0016235 aggresome; GO:0016328 lateral plasma membrane; View More GO Terms; GO:0016342 catenin complex; GO:0016563 transcription activator activity; GO:0022408 negative regulation of cell-cell adhesion; GO:0034329 cell junction assembly; GO:0034332 adherens junction organization; GO:0042993 positive regulation of transcription factor import into nucleus; GO:0043296 apical junction complex; GO:0045295 gamma-catenin binding; GO:0048471 perinuclear region of cytoplasm; GO:0050776 regulation of immune response; GO:0071285 cellular response to lithium ion; GO:0071681 cellular response to indole-3-methanol