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CD324 antibody | AB04/4G6

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Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) gallery image 1
A431 cells stained with 5μg/ml Mouse anti CD324 antibody (VMA00502, green). Hoechst was used as nuclear stain (blue) and imaged with a 20x objective.
Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates probed with CD324 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to CD324 (molecular weight 120 kDa).
Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of CD324 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with Mouse anti CD324 antibody (VMA00502) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and visualized on the ChemiDoc MP with a 29 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.
Anti CD324 Antibody, clone AB04/4G6 (PrecisionAb Monoclonal Antibody) gallery image 4
Normalized signal intensity of CD324 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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IF WB

Mouse anti CD324

Product Type
PrecisionAb Monoclonal
Clone
AB04/4G6
Isotype
IgG2b
Format
Purified
Specificity
CD324

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VMA00502
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IF WB 100 µl loader
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Mouse anti Human CD324 antibody recognizes CD324, also known as cadherin-1, CAM 120/80, E-cadherin, epithelial cadherin or uvomorulin.

The CDH1 gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in CDH1 gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites (provided by RefSeq, Jul 2008).

Mouse anti Human CD324 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti CD324 detects a band of approximately 120 kDa in LNCaP cell lysate.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli derived recombinant protein corresponding to amino acids 338-621 of human CD324
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P12830
Entrez Gene
CDH1
GO Terms
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005913 cell-cell adherens junction
GO:0005925 focal adhesion
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007156 homophilic cell adhesion
GO:0015629 actin cytoskeleton
GO:0016235 aggresome
GO:0016328 lateral plasma membrane
GO:0016342 catenin complex
GO:0016563 transcription activator activity
GO:0022408 negative regulation of cell-cell adhesion
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
GO:0042993 positive regulation of transcription factor import into nucleus
GO:0043296 apical junction complex
GO:0045295 gamma-catenin binding
GO:0048471 perinuclear region of cytoplasm
GO:0050776 regulation of immune response
GO:0071285 cellular response to lithium ion
GO:0071681 cellular response to indole-3-methanol
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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