CD31 antibody | WM59
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Mouse anti Human CD31:StarBright Violet 670
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD31 monoclonal antibody, clone WM59 recognizes the human CD31 antigen, a ~130 kDa single pass type I transmembrane glycoprotein bearing six C2 immunoglobulin domains. CD31 is expressed by all continuous endothelia including arteries, veins and non-sinusoidal capillaries, platelets,granulocytes and some lymphocytes. CD31 is not expressed by discontinuous endothelia such as hepatic sinusoids and splenic red pulp (Muller et al. 1989).CD31 is also known as PECAM-1.
The binding epitope for mouse anti human CD31, clone WM59 has been mapped to the Ig-like domain 2 (Fawcett et al. 1995).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 670 - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 670 400 667
- For research purposes only
- 12 months from date of despatch
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
DO NOT FREEZE.
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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References for CD31 antibody
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Fawcett, J. et al. (1995) Mapping the homotypic binding sites in CD31 and the role of CD31 adhesion in the formation of interendothelial cell contacts.
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Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577.
Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31.
J Immunol. 145 (11): 3889-97.
DeLisser, H.M. et al. (1994) Molecular and functional aspects of PECAM-1/CD31.
Immunol Today. 15 (10): 490-5.
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321 (2): 656-62.
Reedquist, K.A. et al. (2000) The small GTPase, Rap1, mediates CD31-induced integrin adhesion.
J Cell Biol. 148: 1151-8.
Vernon-Wilson, E.F. et al. (2007) CD31 delays phagocyte membrane repolarization to promote efficient binding of apoptotic cells.
J Leukoc Biol. 82: 1278-88.
View The Latest Product References
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Katz SC et al. (2004) Liver sinusoidal endothelial cells are insufficient to activate T cells.
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