CD3 antibody | CD3-12

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Rat anti Human CD3

CD3 antibody, clone CD3-12, recognizes human CD3e, and has a very broad species crossreactivity for the CD3 marker. CD3 is a multimeric protein complex composed of four distinct polypeptide chains that associate with the T cell receptor (TCR).

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
100 Tests/1ml loader

100 Tests/1ml loader

0.1 mg loader

25 µg loader

100 Tests/1ml loader

C F* IF P*
25 µg loader

0.2 mg loader

Rat anti Human CD3 antibody, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.

As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Harbour Porpoise
Cynomolgus monkey
Spotted Hyena
Sea Lion
Amazon Parrot
Mammals Expected from Sequence
Birds Expected from Sequence
Great horned owl (Bubo virginianus)
Amphibia Expected from Sequence
African green monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Pack Size: 100 Tests/1ml
Purified IgG conjugated to Alexa Fluor 488 - liquid
Pack Size: 100 Tests/1ml
Purified IgG - conjugated to Alexa Fluor®647 - liquid
Pack Size: 0.1 mg, 25 µg
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Pack Size: 100 Tests/1ml
Purified IgG conjugated to Pacific Blue - liquid
Pack Size: 25 µg, 0.2 mg
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 100 Tests/1ml
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Pack Size: 0.1 mg, 25 µg
0.09%Sodium Azide
1%Bovine Serum Albumin
Pack Size: 25 µg, 0.2 mg
0.09%Sodium Azide
Carrier Free
Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )
Approx. Protein Concentrations
Pack Size: 100 Tests/1ml
IgG concentration 0.05 mg/ml
Pack Size: 100 Tests/1ml
IgG concentration 0.05mg/ml
Pack Size: 0.1 mg, 25 µg
IgG concentration 0.1 mg/ml
Pack Size: 25 µg, 0.2 mg
IgG concentration 1.0 mg/ml

Storage Information

Pack Size: 100 Tests/1ml, 0.1 mg, 25 µg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Pack Size: 25 µg, 0.2 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0031295 T cell costimulation
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
For research purposes only

Applications of CD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/5 Pack Size: 100 Tests/1ml, 0.1 mg, 25 µg
Neat Pack Size: 100 Tests/1ml
1/50 Pack Size: 25 µg, 0.2 mg
1/10 Pack Size: 100 Tests/1ml, 0.1 mg, 25 µg
1/100 Pack Size: 25 µg, 0.2 mg
Immunohistology - Frozen 1/100
Immunohistology - Paraffin 2 1/100
Western Blotting
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm™ (BUF09) for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG1 Negative Control:Alexa Fluor® 488 MCA6004A488 F 100 Tests/1ml loader
Rat IgG1 Negative Control:Alexa Fluor® 647 MCA6004A647 F 100 Tests/1ml loader
Rat IgG1 Negative Control:FITC MCA6004F F 0.1 mg loader
Rat IgG1 Negative Control:Pacific Blue® MCA6004PB F 100 Tests/1ml loader
Rat IgG1 Negative Control MCA6004GA F 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images


Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for CD3 antibody

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Further Reading

  1. Alterio de Goss, M. et al. (1998) Control of cytomegalovirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rests primarily on recipient-derived CD8 T cells.
    J Virol. 72 (10): 7733-44.
  2. Burudi, E.M. et al. (2002) Regulation of indoleamine 2,3-dioxygenase expression in simian immunodeficiency virus-infected monkey brains.
    J Virol. 76 (23): 12233-41.
  3. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

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