CD282 antibody | 11G7

Mouse anti Human CD282:FITC

Product Type
Monoclonal Antibody
Clone
11G7
Isotype
IgG1
Specificity
CD282

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MCA2484F
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Mouse anti Human CD282 antibody, clone 11G7 recognizes the human Toll-like receptor 2 (TLR2), a ~90 kDa type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. TLR2, also known as CD282, is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Mouse anti Human CD282 antibody, clone 11G7 has been reported to block IL-6 cytokine production in response to ara-lipoarabinomannan (araLAM) and tripalmitoyl cysteinyl lipopeptide (Pam3CSK4) (Sandor et al. 2003). Clone 11G7 does not inhibit the production of cytokines induced by zymosan stimulation.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Human Seroblock
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Description Human Seroblock

References for CD282 antibody

  1. Sandor, F. et al. (2003) Importance of extra- and intracellular domains of TLR1 and TLR2 in NFkappa B signaling.
    J Cell Biol. 162 (6): 1099-110.
  2. Zhou, S. et al. (2010) Discovery of a novel TLR2 signaling inhibitor with anti-viral activity.
    Antiviral Res. 87 (3): 295-306.
  3. Royer, P.J. et al. (2010) The mannose receptor mediates the uptake of diverse native allergens by dendritic cells and determines allergen-induced T cell polarization through modulation of IDO activity.
    J Immunol. 185 (3): 1522-31.
  4. Chen, E.S. et al. (2010) Serum amyloid A regulates granulomatous inflammation in sarcoidosis through Toll-like receptor-2.
    Am J Respir Crit Care Med. 181 (4): 360-73.
  5. Nguyen, D.N. et al. (2016) Oral antibiotics increase blood neutrophil maturation and reduce bacteremia and necrotizing enterocolitis in the immediate postnatal period of preterm pigs.
    Innate Immun. 22 (1): 51-62.
  6. Royer, P.J. et al. (2010) The mannose receptor mediates the uptake of diverse native allergens by dendritic cells and determines allergen-induced T cell polarization through modulation of IDO activity.
    J Immunol. 185 (3): 1522-31.

Synonyms
TLR2
RRID
AB_872045
UniProt
O60603
Entrez Gene
TLR2
GO Terms
GO:0001875 lipopolysaccharide receptor activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005737 cytoplasm
GO:0006917 induction of apoptosis
GO:0006954 inflammatory response
GO:0007252 I-kappaB phosphorylation
GO:0008063 Toll signaling pathway
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0030177 positive regulation of Wnt receptor signaling pathway
GO:0032722 positive regulation of chemokine production
GO:0032728 positive regulation of interferon-beta production
GO:0032735 positive regulation of interleukin-12 production
GO:0032741 positive regulation of interleukin-18 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032757 positive regulation of interleukin-8 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0034123 positive regulation of toll-like receptor signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0042495 detection of triacyl bacterial lipopeptide
GO:0042496 detection of diacyl bacterial lipopeptide
GO:0042497 triacyl lipopeptide binding
GO:0042834 peptidoglycan binding
GO:0045087 innate immune response
GO:0046982 protein heterodimerization activity
GO:0050729 positive regulation of inflammatory response
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051637 Gram-positive bacterial cell surface binding
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
GO:0071223 cellular response to lipoteichoic acid
GO:0071726 cellular response to diacyl bacterial lipopeptide
GO:0071727 cellular response to triacyl bacterial lipopeptide
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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