CD23 antibody | D3.6
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F ResetMouse anti Human CD23:FITC
- Product Type
- Monoclonal Antibody
- Clone
- D3.6
- Isotype
- IgG2a
- Specificity
- CD23
Antibody Quality and 
Unrivaled 
| Mouse anti Human CD23 antibody, clone D3.6 recognizes CD23 also known as FcƐRII (Fc epsilon Receptor II). CD23 is a ~45 kDa cell surface glycoprotein that acts as a low affinity receptor for IgE. CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Immunoprecipitated CD23 antigen from purified plasma membranes from EBV-transformed human lymphoblastoid B cell lines
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
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| Description | Mouse IgG2a Negative Control:FITC | ||||||
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| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
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| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
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| Description | Human Seroblock | ||||||
References for CD23 antibody
-
Thompson, S.A. et al. (2010) B-cell reconstitution and BAFF after alemtuzumab (Campath-1H) treatment of multiple sclerosis.
J Clin Immunol. 30 (1): 99-105.
- Synonyms
- FceRII
- RRID
- AB_323187
- UniProt
- P06734
- Entrez Gene
- FCER2
- GO Terms
- GO:0005576 extracellular region
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0005529 sugar binding
- GO:0019863 IgE binding
- GO:0051000 positive regulation of nitric-oxide synthase activity
- GO:0051712 positive regulation of killing of cells of other organism
- GO:0051773 positive regulation of nitric-oxide synthase 2 biosynthetic process
- View More GO Terms
View more products with CD23 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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