CD23 antibody | D3.6

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Mouse anti Human CD23:FITC

Product Type
Monoclonal Antibody
Clone
D3.6
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1931F F 0.1 mg
Mouse anti Human CD23 antibody, clone D3.6 recognizes CD23 also known as FcƐRII (Fc epsilon Receptor II). CD23 is a ~45 kDa cell surface glycoprotein that acts as a low affinity receptor for IgE.

CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Immunoprecipitated CD23 antigen from purified plasma membranes from EBV-transformed human lymphoblastoid B cell lines
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P06734 Related reagents
Entrez Gene
FCER2 Related reagents
GO Terms
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0005529 sugar binding
GO:0019863 IgE binding
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051712 positive regulation of killing of cells of other organism
GO:0051773 positive regulation of nitric-oxide synthase 2 biosynthetic process
Regulatory
For research purposes only

Applications of CD23 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD23 antibody

  1. Thompson, S.A. et al. (2010) B-cell reconstitution and BAFF after alemtuzumab (Campath-1H) treatment of multiple sclerosis.
    J Clin Immunol. 30 (1): 99-105.